Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models

Aims. According to the need for the development of new anticancer agents, we have synthetized novel bioactive compounds and aimed to determine their antitumor action. Materials & Methods. We describe in vitro studies evaluating the effect of 35 novel chemical compounds on two triple negative...

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Detalles Bibliográficos
Autores principales: Giolito, Maria Virginia, Camacho, Cristian M., Martinez-Amezaga, Maitena, Traficante, Carla Inés, Giordano, Rocío A, Cornier, Patricia G., Mata, Ernesto G., Delpiccolo, Carina M.L., Boggián, Dora G., Del Giúdice, Antonela, Mainetti, Leandro Ernesto, Scharovsky, Olga Graciela, Rozados, Viviana R., Rico, María José
Lenguaje:English
Publicado: Dataverse Prueba 2020
Materias:
Medicine, Health and Life Sciences
Ciencias médicas y de la salud
Antitumor effect
Chemical agents
In vitro assays
Triple negative mammary adenocarcinoma
Acceso en línea:https://hdl.handle.net/20.500.12769/FK2/GQEXAS
http://hdl.handle.net/2133/19264
http://hdl.handle.net/2133/19264
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19264
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language English
orig_language_str_mv English
topic Medicine, Health and Life Sciences
Ciencias médicas y de la salud
Antitumor effect
Chemical agents
In vitro assays
Triple negative mammary adenocarcinoma
spellingShingle Medicine, Health and Life Sciences
Ciencias médicas y de la salud
Antitumor effect
Chemical agents
In vitro assays
Triple negative mammary adenocarcinoma
Giolito, Maria Virginia
Camacho, Cristian M.
Martinez-Amezaga, Maitena
Traficante, Carla Inés
Giordano, Rocío A
Cornier, Patricia G.
Mata, Ernesto G.
Delpiccolo, Carina M.L.
Boggián, Dora G.
Del Giúdice, Antonela
Mainetti, Leandro Ernesto
Scharovsky, Olga Graciela
Rozados, Viviana R.
Rico, María José
Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models
topic_facet Medicine, Health and Life Sciences
Ciencias médicas y de la salud
Antitumor effect
Chemical agents
In vitro assays
Triple negative mammary adenocarcinoma
description Aims. According to the need for the development of new anticancer agents, we have synthetized novel bioactive compounds and aimed to determine their antitumor action. Materials & Methods. We describe in vitro studies evaluating the effect of 35 novel chemical compounds on two triple negative murine mammary adenocarcinoma tumors. Results & Conclusions. Three compounds were selected because of their high antitumor activity and their low toxicity to normal cells. Their effect on tumor cells apoptosis, clonogenicity and migratory capacity, were determined. We found that the selected compounds showed inhibition of viability and clonogenic capacity, and promotion of apoptosis. They also decreased the migratory capacity of tumor cells. The results obtained suggest the likelihood of their future use as antitumor and/or antimetastatic agents SM1: Methodology and characterization of the chemical compounds SM2: Chemical structure of the compounds belonging to each family SM3: Effect of the compounds (100 µM) on 4T1 cells viability. Cells were incubated for 36 hs in complete medium with 100 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate. SM4: Effect of the compounds (75 µM) on 4T1 cells viability. Cells were incubated for 36 hs in complete medium with 75 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate. SM5: Effect of the compounds (50 and 25 µM) on 4T1 cells viability. A) Cells were incubated for 36 hs in complete medium with 50 µM of each compound; B) Cells were incubated for 36 hs in complete medium with 25 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate. SM6: The percent of viability of the selected compounds at 25 µM
author Giolito, Maria Virginia
Camacho, Cristian M.
Martinez-Amezaga, Maitena
Traficante, Carla Inés
Giordano, Rocío A
Cornier, Patricia G.
Mata, Ernesto G.
Delpiccolo, Carina M.L.
Boggián, Dora G.
Del Giúdice, Antonela
Mainetti, Leandro Ernesto
Scharovsky, Olga Graciela
Rozados, Viviana R.
Rico, María José
author_facet Giolito, Maria Virginia
Camacho, Cristian M.
Martinez-Amezaga, Maitena
Traficante, Carla Inés
Giordano, Rocío A
Cornier, Patricia G.
Mata, Ernesto G.
Delpiccolo, Carina M.L.
Boggián, Dora G.
Del Giúdice, Antonela
Mainetti, Leandro Ernesto
Scharovsky, Olga Graciela
Rozados, Viviana R.
Rico, María José
author_sort Giolito, Maria Virginia
title Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models
title_short Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models
title_full Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models
title_fullStr Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models
title_full_unstemmed Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models
title_sort supplementary materials - antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models
publisher Dataverse Prueba
publishDate 2020
url https://hdl.handle.net/20.500.12769/FK2/GQEXAS
http://hdl.handle.net/2133/19264
http://hdl.handle.net/2133/19264
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