Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
Leaf senescence is a concerted physiological process involving controlled degradation of cellular structures and reallocation of breakdown products to other plant organs. It is accompanied by increased production of reactive oxygen species (ROS) that are proposed to signal cell death, although both...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/2133/18694 http://hdl.handle.net/2133/18694 |
| Aporte de: |
| id |
I15-R121-2133-18694 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de Rosario |
| institution_str |
I-15 |
| repository_str |
R-121 |
| collection |
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Senescence Chloroplasts Reactive oxygen species Redox poise Flavodoxin Transgenic plants Tobacco |
| spellingShingle |
Senescence Chloroplasts Reactive oxygen species Redox poise Flavodoxin Transgenic plants Tobacco Mayta, Martín L. Lodeyro, Anabella F. Guiamet, Juan J. Tognetti, Vanesa B. Melzer, Michael Hajirezaei, Mohammad-Reza Carrillo, Néstor Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves |
| topic_facet |
Senescence Chloroplasts Reactive oxygen species Redox poise Flavodoxin Transgenic plants Tobacco |
| description |
Leaf senescence is a concerted physiological process involving controlled degradation of cellular structures and reallocation of breakdown products to other plant organs. It is accompanied by increased production of reactive oxygen species (ROS) that are proposed to signal cell death, although both the origin and the precise role of ROS in the execution of this developmental program are still poorly understood. To investigate the contribution of chloroplast-associated ROS to natural leaf senescence, we used tobacco plants expressing a plastid-targeted flavodoxin, an electron shuttle flavoprotein present in prokaryotes and algae. When expressed in plants, flavodoxin specifically prevents ROS formation in chloroplasts during stress situations. Senescence symptoms were significantly mitigated in these transformants, with decreased accumulation of chloroplastic ROS and differential preservation of chlorophylls, carotenoids, protein contents, cell and chloroplast structures, membrane integrity and cell viability. Flavodoxin also improved maintenance of chlorophyll-protein complexes, photosynthetic electron flow, CO2 assimilation, central metabolic routes and levels of bioactive cytokinins and auxins in aging leaves. Delayed induction of senescence-associated genes indicates that the entire genetic program of senescence was affected by flavodoxin. The results suggest that ROS generated in chloroplasts are involved in the regulation of natural leaf senescence. |
| format |
article artículo publishedVersion |
| author |
Mayta, Martín L. Lodeyro, Anabella F. Guiamet, Juan J. Tognetti, Vanesa B. Melzer, Michael Hajirezaei, Mohammad-Reza Carrillo, Néstor |
| author_facet |
Mayta, Martín L. Lodeyro, Anabella F. Guiamet, Juan J. Tognetti, Vanesa B. Melzer, Michael Hajirezaei, Mohammad-Reza Carrillo, Néstor |
| author_sort |
Mayta, Martín L. |
| title |
Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves |
| title_short |
Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves |
| title_full |
Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves |
| title_fullStr |
Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves |
| title_full_unstemmed |
Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves |
| title_sort |
expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves |
| publisher |
Frontiers Media |
| publishDate |
2020 |
| url |
http://hdl.handle.net/2133/18694 http://hdl.handle.net/2133/18694 |
| work_keys_str_mv |
AT maytamartinl expressionofaplastidtargetedflavodoxindecreaseschloroplastreactiveoxygenspeciesaccumulationanddelayssenescenceinagingtobaccoleaves AT lodeyroanabellaf expressionofaplastidtargetedflavodoxindecreaseschloroplastreactiveoxygenspeciesaccumulationanddelayssenescenceinagingtobaccoleaves AT guiametjuanj expressionofaplastidtargetedflavodoxindecreaseschloroplastreactiveoxygenspeciesaccumulationanddelayssenescenceinagingtobaccoleaves AT tognettivanesab expressionofaplastidtargetedflavodoxindecreaseschloroplastreactiveoxygenspeciesaccumulationanddelayssenescenceinagingtobaccoleaves AT melzermichael expressionofaplastidtargetedflavodoxindecreaseschloroplastreactiveoxygenspeciesaccumulationanddelayssenescenceinagingtobaccoleaves AT hajirezaeimohammadreza expressionofaplastidtargetedflavodoxindecreaseschloroplastreactiveoxygenspeciesaccumulationanddelayssenescenceinagingtobaccoleaves AT carrillonestor expressionofaplastidtargetedflavodoxindecreaseschloroplastreactiveoxygenspeciesaccumulationanddelayssenescenceinagingtobaccoleaves |
| bdutipo_str |
Repositorios |
| _version_ |
1764820410621231108 |