The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters

Heme (iron protoporphyrin IX) is an important molecule involved in many biological reactions, including oxygen transport, respiration, photosynthesis and drug detoxification. Trypanosoma cruzi parasites, the etiological agent of Chagas’ disease, take up heme from the environment to supply their nutr...

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Autores principales:	Cupello Peixoto, Mauricio, Fernandes de Souza, Cíntia, Buchensky, Celeste, Rocha Corrêa Soares, Juliana Baptista, Travassos Laranja, Gustavo Augusto, Garcia Pinto Coelho, Marsen, Cricco, Julia Alejandra, Paes, Marcia Cristina
Formato:	article artículo acceptedVersion
Lenguaje:	Inglés
Publicado:	Elsevier 2020
Materias:	Heme Trypanosoma cruzi Porphyrins Heme transport Biological Transport
Acceso en línea:	http://hdl.handle.net/2133/18347 http://hdl.handle.net/2133/18347
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-18347
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institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	Heme Trypanosoma cruzi Porphyrins Heme transport Biological Transport
spellingShingle	Heme Trypanosoma cruzi Porphyrins Heme transport Biological Transport Cupello Peixoto, Mauricio Fernandes de Souza, Cíntia Buchensky, Celeste Rocha Corrêa Soares, Juliana Baptista Travassos Laranja, Gustavo Augusto Garcia Pinto Coelho, Marsen Cricco, Julia Alejandra Paes, Marcia Cristina The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
topic_facet	Heme Trypanosoma cruzi Porphyrins Heme transport Biological Transport
description	Heme (iron protoporphyrin IX) is an important molecule involved in many biological reactions, including oxygen transport, respiration, photosynthesis and drug detoxification. Trypanosoma cruzi parasites, the etiological agent of Chagas’ disease, take up heme from the environment to supply their nutritional needs because they do not synthesize this cofactor. However, the mechanisms involved in heme transport across biological membranes are poorly understood. Indeed, in T. cruzi, no heme transporter has yet been characterized. In the present work, we evaluate the heme uptake processes by T. cruzi epimastigotes using fluorescent heme-analogues. Heme uptake decreased significantly when cells were pretreated with different concentrations of SnPPIX, PdMPIX or ZnMPIX, this observed competition suggests that they are taken up by the same transport system. We studied the growth behavior of epimastigotes using the same heme-analogues and the treatments with SnPPIX or PdMPIX impaired cell growth but when heme was added to the culture medium the observed inhibition was partially reversed. In addition, we tested how the heme uptake processes are affected by the presence of different transporter inhibitors. When the cells were treated with inhibitors and then incubated with heme, heme uptake decreased significantly for all treatments. These results constitute a strong indication for the existence of a protein associated with porphyrin transport in T. cruzi, possibly ATP-binding cassette transporters (ABC-transporter).
format	article artículo acceptedVersion
author	Cupello Peixoto, Mauricio Fernandes de Souza, Cíntia Buchensky, Celeste Rocha Corrêa Soares, Juliana Baptista Travassos Laranja, Gustavo Augusto Garcia Pinto Coelho, Marsen Cricco, Julia Alejandra Paes, Marcia Cristina
author_facet	Cupello Peixoto, Mauricio Fernandes de Souza, Cíntia Buchensky, Celeste Rocha Corrêa Soares, Juliana Baptista Travassos Laranja, Gustavo Augusto Garcia Pinto Coelho, Marsen Cricco, Julia Alejandra Paes, Marcia Cristina
author_sort	Cupello Peixoto, Mauricio
title	The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_short	The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_full	The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_fullStr	The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_full_unstemmed	The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_sort	heme uptake process in trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of abc transporters
publisher	Elsevier
publishDate	2020
url	http://hdl.handle.net/2133/18347 http://hdl.handle.net/2133/18347
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The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters

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