The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake

Trypanosoma cruzi, the etiological agent of Chagas' disease, presents nutritional requirements for several metabolites. It requires heme for the biosynthesis of several heme-proteins involved in essential metabolic pathways like mitochondrial cytochromes and respiratory complexes, as well as en...

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Autores principales: Merli, Marcelo Luciano, Pagura, Lucas, Hernández, Josefina, Barisón, María Julia, Pral, Elizabeth M. F., Silber, Ariel M., Cricco, Julia Alejandra
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Public Library of Science PLOS 2020
Materias:
Trypanosoma cruzi
Chagas' disease
Trypanosoma cruzi Protein TcHTE
Heme uptake
Parasitic life cycles
Acceso en línea:http://hdl.handle.net/2133/18325
http://hdl.handle.net/2133/18325
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-18325
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Trypanosoma cruzi
Chagas' disease
Trypanosoma cruzi Protein TcHTE
Heme uptake
Parasitic life cycles
spellingShingle Trypanosoma cruzi
Chagas' disease
Trypanosoma cruzi Protein TcHTE
Heme uptake
Parasitic life cycles
Merli, Marcelo Luciano
Pagura, Lucas
Hernández, Josefina
Barisón, María Julia
Pral, Elizabeth M. F.
Silber, Ariel M.
Cricco, Julia Alejandra
The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake
topic_facet Trypanosoma cruzi
Chagas' disease
Trypanosoma cruzi Protein TcHTE
Heme uptake
Parasitic life cycles
description Trypanosoma cruzi, the etiological agent of Chagas' disease, presents nutritional requirements for several metabolites. It requires heme for the biosynthesis of several heme-proteins involved in essential metabolic pathways like mitochondrial cytochromes and respiratory complexes, as well as enzymes involved in the biosynthesis of sterols and unsaturated fatty acids. However, this parasite lacks a complete route for its synthesis. In view of these facts, T. cruzi has to incorporate heme from the environment during its life cycle. In other words, their hosts must supply the heme for heme-protein synthesis. Although the acquisition of heme is a fundamental issue for the parasite’s replication and survival, how this cofactor is imported and distributed is poorly understood. In this work, we used different fluorescent heme analogs to explore heme uptake along the different life-cycle stages of T. cruzi, showing that this parasite imports it during its replicative stages: the epimastigote in the insect vector and the intracellular amastigote in the mammalian host. Also, we identified and characterized a T. cruzi protein (TcHTE) with 55% of sequence similarity to LHR1 (protein involved in L. amazonensis heme transport), which is located in the flagellar pocket, where the transport of nutrients proceeds in trypanosomatids. We postulate TcHTE as a protein involved in improving the efficiency of the heme uptake or trafficking in T. cruzi.
format article
artículo
publishedVersion
author Merli, Marcelo Luciano
Pagura, Lucas
Hernández, Josefina
Barisón, María Julia
Pral, Elizabeth M. F.
Silber, Ariel M.
Cricco, Julia Alejandra
author_facet Merli, Marcelo Luciano
Pagura, Lucas
Hernández, Josefina
Barisón, María Julia
Pral, Elizabeth M. F.
Silber, Ariel M.
Cricco, Julia Alejandra
author_sort Merli, Marcelo Luciano
title The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake
title_short The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake
title_full The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake
title_fullStr The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake
title_full_unstemmed The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake
title_sort trypanosoma cruzi protein tchte is critical for heme uptake
publisher Public Library of Science PLOS
publishDate 2020
url http://hdl.handle.net/2133/18325
http://hdl.handle.net/2133/18325
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