The Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiae

Trypanosoma cruzi, the etiologic agent for Chagas' disease, has requirements for several cofactors, one of which is heme. Because this organism is unable to synthesize heme, which serves as a prosthetic group for several heme proteins (including the respiratory chain complexes), it therefore mu...

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Autores principales: Buchensky, Celeste, Almirón, Paula, Suarez Mantilla, Brian, Silber, Ariel M., Cricco, Julia Alejandra
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Oxford University Press 2020
Materias:
Trypanosoma cruzi
heme A
cytochrome c oxidase
mitochondrial proteins
Chagas' disease
Acceso en línea:http://hdl.handle.net/2133/18322
http://hdl.handle.net/2133/18322
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-18322
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Trypanosoma cruzi
heme A
cytochrome c oxidase
mitochondrial proteins
Chagas' disease
spellingShingle Trypanosoma cruzi
heme A
cytochrome c oxidase
mitochondrial proteins
Chagas' disease
Buchensky, Celeste
Almirón, Paula
Suarez Mantilla, Brian
Silber, Ariel M.
Cricco, Julia Alejandra
The Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiae
topic_facet Trypanosoma cruzi
heme A
cytochrome c oxidase
mitochondrial proteins
Chagas' disease
description Trypanosoma cruzi, the etiologic agent for Chagas' disease, has requirements for several cofactors, one of which is heme. Because this organism is unable to synthesize heme, which serves as a prosthetic group for several heme proteins (including the respiratory chain complexes), it therefore must be acquired from the environment. Considering this deficiency, it is an open question as to how heme A, the essential cofactor for eukaryotic CcO enzymes, is acquired by this parasite. In the present work, we provide evidence for the presence and functionality of genes coding for heme O and heme A synthases, which catalyze the synthesis of heme O and its conversion into heme A, respectively. The functions of these T. cruzi proteins were evaluated using yeast complementation assays, and the mRNA levels of their respective genes were analyzed at the different T. cruzi life stages. It was observed that the amount of mRNA coding for these proteins changes during the parasite life cycle, suggesting that this variation could reflect different respiratory requirements in the different parasite life stages.
format article
artículo
publishedVersion
author Buchensky, Celeste
Almirón, Paula
Suarez Mantilla, Brian
Silber, Ariel M.
Cricco, Julia Alejandra
author_facet Buchensky, Celeste
Almirón, Paula
Suarez Mantilla, Brian
Silber, Ariel M.
Cricco, Julia Alejandra
author_sort Buchensky, Celeste
title The Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiae
title_short The Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiae
title_full The Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiae
title_fullStr The Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiae
title_full_unstemmed The Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiae
title_sort trypanosoma cruzi proteins tccox10 and tccox15 catalyze the formation of heme a in the yeast saccharomyces cerevisiae
publisher Oxford University Press
publishDate 2020
url http://hdl.handle.net/2133/18322
http://hdl.handle.net/2133/18322
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