Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae

The complex life cycle of Trichinella spiralis includes the migration of newborn larvae through the bloodstream to their encystment in muscle. The parasite establishes an intimate contact with the erythrocytes of the host both during the migration of the newborn larvae and when encysting, as this pa...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ponce de León, Patricia, Bellini, Mariana, Castellini, Horacio V., Riquelme, Bibiana Doris
Formato:	article artículo acceptedVersion
Lenguaje:	Inglés
Publicado:	Cambridge University Press 2020
Materias:	Erythrocyte aggregation Erythrocyte viscoelasticity Trichinella spiralis muscle larvae
Acceso en línea:	http://hdl.handle.net/2133/17697 http://hdl.handle.net/2133/17697
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-17697
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	Erythrocyte aggregation Erythrocyte viscoelasticity Trichinella spiralis muscle larvae
spellingShingle	Erythrocyte aggregation Erythrocyte viscoelasticity Trichinella spiralis muscle larvae Ponce de León, Patricia Bellini, Mariana Castellini, Horacio V. Riquelme, Bibiana Doris Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae
topic_facet	Erythrocyte aggregation Erythrocyte viscoelasticity Trichinella spiralis muscle larvae
description	The complex life cycle of Trichinella spiralis includes the migration of newborn larvae through the bloodstream to their encystment in muscle. The parasite establishes an intimate contact with the erythrocytes of the host both during the migration of the newborn larvae and when encysting, as this parasite causes intense vascularization in the muscle cell. The goal of this work was to study the effects of various concentrations of T. spiralis muscle larvae (ML) on erythrocyte membranes. The treatment was performed by incubating human erythrocytes with equal volume of different concentrations of ML for 30 minutes, with controlled agitation (37°C). The control erythrocytes (with no contact with the larvae) were incubated in the same way with an equal volume of physiological solution. To evaluate the alterations to the erythrocytes by the action of the larvae and in the respective controls, an Erythrocyte Rheometer and a Digital Image Analysis technique were used. The results indicated that when the larval concentration was higher, the aggregation and erythrocyte membrane alterations were also higher. Also, the erythrocyte deformability index and the erythrocyte elasticity increased. The values of isolated cell coefficient varied from 0.51 in the treatment with 100 larvae/ml to 0.91 in the incubation with 1000 larvae/ml. This experiment shows that T. spiralis muscle larvae affect significantly the red blood cell aggregation and the erythrocyte viscoelastic properties.
format	article artículo acceptedVersion
author	Ponce de León, Patricia Bellini, Mariana Castellini, Horacio V. Riquelme, Bibiana Doris
author_facet	Ponce de León, Patricia Bellini, Mariana Castellini, Horacio V. Riquelme, Bibiana Doris
author_sort	Ponce de León, Patricia
title	Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae
title_short	Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae
title_full	Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae
title_fullStr	Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae
title_full_unstemmed	Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae
title_sort	alterations of the erythrocyte membrane by in vitro action of trichinella spiralis muscle larvae
publisher	Cambridge University Press
publishDate	2020
url	http://hdl.handle.net/2133/17697 http://hdl.handle.net/2133/17697
work_keys_str_mv	AT poncedeleonpatricia alterationsoftheerythrocytemembranebyinvitroactionoftrichinellaspiralismusclelarvae AT bellinimariana alterationsoftheerythrocytemembranebyinvitroactionoftrichinellaspiralismusclelarvae AT castellinihoraciov alterationsoftheerythrocytemembranebyinvitroactionoftrichinellaspiralismusclelarvae AT riquelmebibianadoris alterationsoftheerythrocytemembranebyinvitroactionoftrichinellaspiralismusclelarvae
bdutipo_str	Repositorios
_version_	1764820409532809216

Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae

Ejemplares similares