Preliminary study of alterations in human red blood cells by irradiation with high energy photons
Introduction: Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells...
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| Autores principales: | , , , , , , , , |
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| Formato: | conferenceObject documento de conferencia publishedVersion |
| Lenguaje: | Inglés |
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Blucher
2019
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| Acceso en línea: | http://hdl.handle.net/2133/17140 http://hdl.handle.net/2133/17140 |
| Aporte de: |
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I15-R121-2133-17140 |
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dspace |
| institution |
Universidad Nacional de Rosario |
| institution_str |
I-15 |
| repository_str |
R-121 |
| collection |
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Red Blood Cells Erythrocytes High Energy Photons Radiation Effects |
| spellingShingle |
Red Blood Cells Erythrocytes High Energy Photons Radiation Effects Riquelme, Bibiana Doris Estrada, Ezequiel Castellini, Horacio V. Acosta, Andrea Chinelatto, Alejandro Tack, Ivan Borraz, Javier Di Tullio, Liliana Galassi, Mariel Elisa Preliminary study of alterations in human red blood cells by irradiation with high energy photons |
| topic_facet |
Red Blood Cells Erythrocytes High Energy Photons Radiation Effects |
| description |
Introduction: Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008).
In a recent work (AlZahrani K. et al., 2017), nanoestructural changes in the RBC membrane at different doses of gamma irradiation were observed using atomic force microscopy. The images shown that the roughness of the cell membrane increased dramatically with increasing doses, affecting their biophysical properties. However, more research is needed to understand the effects of gamma irradiation on the mechanical and adhesion properties of RBC. For this reason, in the present work we set out to measure the mechanical and aggregation parameters of human red blood cells exposed to gamma photons in different doses in order to determine the possible alterations due to radiation. |
| author2 |
D'Arrigo, Mabel. Colaboración en preparación de muestras biológicas |
| author_facet |
D'Arrigo, Mabel. Colaboración en preparación de muestras biológicas Riquelme, Bibiana Doris Estrada, Ezequiel Castellini, Horacio V. Acosta, Andrea Chinelatto, Alejandro Tack, Ivan Borraz, Javier Di Tullio, Liliana Galassi, Mariel Elisa |
| format |
conferenceObject documento de conferencia publishedVersion |
| author |
Riquelme, Bibiana Doris Estrada, Ezequiel Castellini, Horacio V. Acosta, Andrea Chinelatto, Alejandro Tack, Ivan Borraz, Javier Di Tullio, Liliana Galassi, Mariel Elisa |
| author_sort |
Riquelme, Bibiana Doris |
| title |
Preliminary study of alterations in human red blood cells by irradiation with high energy photons |
| title_short |
Preliminary study of alterations in human red blood cells by irradiation with high energy photons |
| title_full |
Preliminary study of alterations in human red blood cells by irradiation with high energy photons |
| title_fullStr |
Preliminary study of alterations in human red blood cells by irradiation with high energy photons |
| title_full_unstemmed |
Preliminary study of alterations in human red blood cells by irradiation with high energy photons |
| title_sort |
preliminary study of alterations in human red blood cells by irradiation with high energy photons |
| publisher |
Blucher |
| publishDate |
2019 |
| url |
http://hdl.handle.net/2133/17140 http://hdl.handle.net/2133/17140 |
| work_keys_str_mv |
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Repositorios |
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