A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers

Paspalum notatum Flu¨ gge is a warmseason forage grass with mainly diploid (2n = 20) and autotetraploid (2n = 40) representatives. Diploid races reproduce sexually and require crosspollination due to a self-incompatible mating system, while autotetraploids reproduce by aposporous apomixis. The...

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Autores principales: Stein, Juliana, Pessino, Silvina Claudia, Martínez, Eric Javier, Rodríguez, María Pía, Siena, Lorena Adelina, Quarin, Camilo Luis, Ortiz, Juan Pablo Amelio
Formato: article artículo publishedVersion Material Didáctico
Lenguaje:Inglés
Publicado: Springer Science+Business Media B.V. 2018
Materias:
Apospory
Mapping in autopolyploids
Paspalum notatum
Single dose markers
Acceso en línea:http://hdl.handle.net/2133/13791
http://hdl.handle.net/2133/13791
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-13791
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Apospory
Mapping in autopolyploids
Paspalum notatum
Single dose markers
spellingShingle Apospory
Mapping in autopolyploids
Paspalum notatum
Single dose markers
Stein, Juliana
Pessino, Silvina Claudia
Martínez, Eric Javier
Rodríguez, María Pía
Siena, Lorena Adelina
Quarin, Camilo Luis
Ortiz, Juan Pablo Amelio
A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers
topic_facet Apospory
Mapping in autopolyploids
Paspalum notatum
Single dose markers
description Paspalum notatum Flu¨ gge is a warmseason forage grass with mainly diploid (2n = 20) and autotetraploid (2n = 40) representatives. Diploid races reproduce sexually and require crosspollination due to a self-incompatible mating system, while autotetraploids reproduce by aposporous apomixis. The objectives of this work were to develop a genetic linkage map of Paspalum notatum Flu¨gge at the tetraploid level, identify the linkage/s group/s associated with apomixis and carry out a general characterization of its mode of inheritance. A pseudo test-cross F1 family of 113 individuals segregating for the mode of reproduction was obtained by crossing a synthetic completely sexual tetraploid plant (Q4188) as female parent with a natural aposporous individual (Q4117) as pollen donor. Map construction was based on single-dose markers (SDAFs) segregating from both parents. Two linkage maps (female and male) were constructed. Within each map, homologous groups were assembled by detecting repulsion-phase linked SDAFs. Putative Q4188 and Q4117 homolog groups were identified by mapping shared single dose markers (BSDF). The Q4188 map consisted of 263 markers distributed on 26 co-segregation groups over a total genetic distance of 1.590.6 cM, while the Q4117 map contained 216 loci dispersed on 39 co-segregation groups along 2.265.7 cM, giving an estimated genome coverage of 88% and 83%, respectively. Seven and 12 putative homologous chromosomes were detected within Q4188 and Q4117 maps, respectively. Afterward, ten female and male homologous chromosomes were identified by mapping BSDFs. In the Q4117 map, a single linkage group was associated with apospory. It was characterized by restriction in recombination and preferential chromosome pairing. A BPSD marker mapping within this group allowed the detection of the female homolog and the putative four male groups of the set carrying apospory.
format article
artículo
publishedVersion
Material Didáctico
author Stein, Juliana
Pessino, Silvina Claudia
Martínez, Eric Javier
Rodríguez, María Pía
Siena, Lorena Adelina
Quarin, Camilo Luis
Ortiz, Juan Pablo Amelio
author_facet Stein, Juliana
Pessino, Silvina Claudia
Martínez, Eric Javier
Rodríguez, María Pía
Siena, Lorena Adelina
Quarin, Camilo Luis
Ortiz, Juan Pablo Amelio
author_sort Stein, Juliana
title A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers
title_short A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers
title_full A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers
title_fullStr A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers
title_full_unstemmed A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers
title_sort genetic map of tetraploid paspalum notatum flügge (bahiagrass) based on single-dose molecular markers
publisher Springer Science+Business Media B.V.
publishDate 2018
url http://hdl.handle.net/2133/13791
http://hdl.handle.net/2133/13791
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