Evaluation of SRAP markers for mapping of Pisum sativum L.
Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 was generated. A tot...
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| Autores principales: | , , , , |
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| Formato: | article artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Brazilian Society of Plant Breeding
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/2133/13789 http://hdl.handle.net/2133/13789 |
| Aporte de: |
| Sumario: | Linkage maps have become important tools for genetic studies. With
the aim of evaluating the SRAP (sequence-related amplified polymorphism)
technique for linkage mapping in Pisum sativum L., a F2
mapping population
derived from an initial cross between cvs. DDR11 and Zav25 was generated. A
total of 25 SRAP primer combinations were evaluated in 45 F2
plants and both
parental lines, generating 208 polymorphic bands/markers. The markers were
analyzed by the chi-square goodness-of-fit test to check the expected Mendelian
segregation ratio. The resulting linkage map consists of 112 genetic markers
distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of
the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers.
The linkage map developed in this study indicates that the SRAP marker system
could be applied to mapping studies of pea. |
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