Evaluation of SRAP markers for mapping of Pisum sativum L.

Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 was generated. A tot...

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Detalles Bibliográficos
Autores principales: Guindon, María Fernanda, Martin, Eugenia Alejandra, Zayas, Aldana, Cointry, Enrique, Cravero, Vanina
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Brazilian Society of Plant Breeding 2018
Materias:
Pea
plant breeding
linkage map
F2 population
molecular markers
Acceso en línea:http://hdl.handle.net/2133/13789
http://hdl.handle.net/2133/13789
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-13789
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Pea
plant breeding
linkage map
F2 population
molecular markers
spellingShingle Pea
plant breeding
linkage map
F2 population
molecular markers
Guindon, María Fernanda
Martin, Eugenia Alejandra
Zayas, Aldana
Cointry, Enrique
Cravero, Vanina
Evaluation of SRAP markers for mapping of Pisum sativum L.
topic_facet Pea
plant breeding
linkage map
F2 population
molecular markers
description Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 was generated. A total of 25 SRAP primer combinations were evaluated in 45 F2 plants and both parental lines, generating 208 polymorphic bands/markers. The markers were analyzed by the chi-square goodness-of-fit test to check the expected Mendelian segregation ratio. The resulting linkage map consists of 112 genetic markers distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers. The linkage map developed in this study indicates that the SRAP marker system could be applied to mapping studies of pea.
format article
artículo
publishedVersion
author Guindon, María Fernanda
Martin, Eugenia Alejandra
Zayas, Aldana
Cointry, Enrique
Cravero, Vanina
author_facet Guindon, María Fernanda
Martin, Eugenia Alejandra
Zayas, Aldana
Cointry, Enrique
Cravero, Vanina
author_sort Guindon, María Fernanda
title Evaluation of SRAP markers for mapping of Pisum sativum L.
title_short Evaluation of SRAP markers for mapping of Pisum sativum L.
title_full Evaluation of SRAP markers for mapping of Pisum sativum L.
title_fullStr Evaluation of SRAP markers for mapping of Pisum sativum L.
title_full_unstemmed Evaluation of SRAP markers for mapping of Pisum sativum L.
title_sort evaluation of srap markers for mapping of pisum sativum l.
publisher Brazilian Society of Plant Breeding
publishDate 2018
url http://hdl.handle.net/2133/13789
http://hdl.handle.net/2133/13789
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