Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers

Soybean cultivars carrying the ‘long juvenile trait’ show a delayed flowering response under short day conditions. The incorporation of this character into genotypes of agronomic interest may allow a broader range of sowing dates and latitudes for a single cultivar adaptation. The objective of this...

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Detalles Bibliográficos
Autores principales: Cairo, Carlos Alberto, Stein, Juliana, Delgado, Luciana, Bortolotti, Santiago, Guelman, Sebastián A., Ortiz, Juan Pablo A., Morandi, Eligio
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Kluwer Academic Publishers 2018
Materias:
Glycine max
juvenile locus
molecular markers
RAPD
Acceso en línea:http://hdl.handle.net/2133/13774
http://hdl.handle.net/2133/13774
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-13774
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Glycine max
juvenile locus
molecular markers
RAPD
spellingShingle Glycine max
juvenile locus
molecular markers
RAPD
Cairo, Carlos Alberto
Stein, Juliana
Delgado, Luciana
Bortolotti, Santiago
Guelman, Sebastián A.
Ortiz, Juan Pablo A.
Morandi, Eligio
Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers
topic_facet Glycine max
juvenile locus
molecular markers
RAPD
description Soybean cultivars carrying the ‘long juvenile trait’ show a delayed flowering response under short day conditions. The incorporation of this character into genotypes of agronomic interest may allow a broader range of sowing dates and latitudes for a single cultivar adaptation. The objective of this work was to identify molecular markers linked to the juvenile locus in soybean. Experiments were carried out using two pairs of near isogenic lines (NILs) differing in the presence of the long juvenile trait, and RAPD markers. Four hundred primers were first screened to find polymorphism associated with the trait. Additional differences between NILs were sought by digesting the genomic DNA with five restriction enzymes. Polymorphic fragments detected between NILs were tested for linkage to the juvenile locus in the corresponding F2 segregating populations. Marker bc357-HaeIII was linked (χ2L = 46.316) to the juvenile locus with an estimated recombination frequency of 0.13 ± 0.03 in one of the genetic backgrounds studied. The fragment was cloned, sequenced and converted into a SCAR marker. Moreover, bc357-HaeIII was used as RFLP probe. Both, SCAR and RFLP generated markers linked to the juvenile locus in the two genetic backgrounds analysed. Results presented in this work can be utilised for both, the localisation of the gene associated with the character and for tagging the juvenile trait in soybean breeding programs.
format article
artículo
publishedVersion
author Cairo, Carlos Alberto
Stein, Juliana
Delgado, Luciana
Bortolotti, Santiago
Guelman, Sebastián A.
Ortiz, Juan Pablo A.
Morandi, Eligio
author_facet Cairo, Carlos Alberto
Stein, Juliana
Delgado, Luciana
Bortolotti, Santiago
Guelman, Sebastián A.
Ortiz, Juan Pablo A.
Morandi, Eligio
author_sort Cairo, Carlos Alberto
title Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers
title_short Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers
title_full Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers
title_fullStr Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers
title_full_unstemmed Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers
title_sort tagging the juvenile locus in soybean [glycine max (l.) merr.] with molecular markers
publisher Kluwer Academic Publishers
publishDate 2018
url http://hdl.handle.net/2133/13774
http://hdl.handle.net/2133/13774
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