Attenuation of liver cancer development by oral glycerol supplementation in the rat

PURPOSE:Glycerol usage is increasing in food industry for human and animal nutrition. This study analyzed the impact of glycerol metabolism when orally supplemented during the early stage of rat liver carcinogenesis.METHODS:Wistar rats were subjected to a 2-phase model of hepatocarcinogenesis (initi...

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Detalles Bibliográficos
Autores principales: Capiglioni, Alejo M., Lorenzetti, Florencia, Quiroga, Ariel Darío, Parody, Juan Pablo, Ronco, María Teresa, Pisani, Gerardo Bruno, Carrillo, María Cristina, Ceballos Mancini, María Paula, Alvarez, María de Luján
Otros Autores: Rassetto, Mauricio: asistencia técnica
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Springer 2018
Materias:
Apoptosis
Glycerol
Liver Preneoplasia
Oxidative Stress
Acceso en línea:http://hdl.handle.net/2133/13719
http://hdl.handle.net/2133/13719
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
Descripción
Sumario:PURPOSE:Glycerol usage is increasing in food industry for human and animal nutrition. This study analyzed the impact of glycerol metabolism when orally supplemented during the early stage of rat liver carcinogenesis.METHODS:Wistar rats were subjected to a 2-phase model of hepatocarcinogenesis (initiated-promoted, IP group). IP animals also received glycerol by gavage (200 mg/kg body weight, IPGly group).RESULTS:Glycerol treatment reduced the volume of preneoplastic lesions by decreasing the proliferative status of liver foci, increasing the expression of p53 and p21 proteins and reducing the expression of cyclin D1 and cyclin-dependent kinase 1. Besides, apoptosis was enhanced in IPGly animals, given by an increment of Bax/Bcl-2 ratio, Bad and PUMA mitochondrial expression, a concomitant increase in cytochrome c release and caspase-3 activation. Furthermore, hepatic levels of glycerol phosphate and markers of oxidative stress were increased in IPGly rats. Oxidative stress intermediates act as intracellular messengers, inducing p53 activation and changes in JNK and Erk signaling pathways, with JNK activation and Erk inhibition.CONCLUSION:The present work provides novel data concerning the preventive actions of glycerol during the development of liver cancer and represents an economically feasible intervention to treat high-risk individuals.

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