Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes

Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purpose...

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Autores principales: Aros, Danilo, Valdés, Santiago, Olate, Eduardo, Infante, Rodrigo
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 2012
Materias:
Acceso en línea:http://bdigital.uncu.edu.ar/4523
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id I11-R84523
record_format dspace
institution Universidad Nacional de Cuyo
institution_str I-11
repository_str R-8
collection Biblioteca Digital
language Inglés
orig_language_str_mv eng
topic Chile
Mutación
Brotación
Mejoramiento genético
Irradiación gamma
Mutation
Sprouting
Breeding
Radio susceptibilidad
spellingShingle Chile
Mutación
Brotación
Mejoramiento genético
Irradiación gamma
Mutation
Sprouting
Breeding
Radio susceptibilidad
Aros, Danilo
Valdés, Santiago
Olate, Eduardo
Infante, Rodrigo
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes
topic_facet Chile
Mutación
Brotación
Mejoramiento genético
Irradiación gamma
Mutation
Sprouting
Breeding
Radio susceptibilidad
description Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purposes and it depends mainly on plant susceptibility. Thus in this study in vitro cultured rhizomes of Alstroemeria aurea were irradiated with a gamma source using different dosages to evaluate the direct effect produced. Damage and number of rhizome sprouting were observed and recorded during 61 days after irradiation. At the end of this period, rhizomes were weighted and mortality was evaluated. Both mortality and weight increased depending on dosage. All irradiated rhizomes showed early sprouting in comparison with control (0 Gy) and no significant difference in final number of shoots after 61 days among irradiated treatments was observed. Bleaching and necrosis was observed in all irradiated rhizomes and was more evident at higher doses. LD50 was established at about 40 Gy and the optimum dosage to induce mutation was suggested between 2.5 and 5 Gy, when the growth was reduced in 50%, and probably this dosage could be used for breeding purposes.
format article
Artículo
Artículo
publishedVersion
author Aros, Danilo
Valdés, Santiago
Olate, Eduardo
Infante, Rodrigo
author_facet Aros, Danilo
Valdés, Santiago
Olate, Eduardo
Infante, Rodrigo
author_sort Aros, Danilo
title Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes
title_short Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes
title_full Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes
title_fullStr Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes
title_full_unstemmed Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes
title_sort gamma irradiation on alstroemeria aurea g. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes
publisher Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
publishDate 2012
url http://bdigital.uncu.edu.ar/4523
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