Control of Rhizoctonia solani with extracts from Ovidia andina

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New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen. Powdered dried leaves and stems were extracted by maceration with dichloromethane (CH2Cl2), methanol and ethanol. An aqueous ex...

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Autores principales: Tito Mansilla, Javier, Tarcaya, Verónica P., Cufre, Ingrid M., Fabrizio, María C., Wright, Eduardo R., Broussalis, Adriana M., Rivera, Marta C.
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 2018
Materias:
Rhizoctonia solani
Extractos
Ovidia andina
Hongos patógenos
Cumarinas
Flavonoides
Control de enfermedades de plantas
Active fractions
Bioguided fractionation
CH2Cl2 extract
Coumarins and flavonoids characterization
Fungal growth
Hongos patógenos de suelo
Fracciones activas
Extracto CH2Cl2
Acceso en línea:http://bdigital.uncu.edu.ar/12070
Aporte de:
Biblioteca Digital de Universidad Nacional de Cuyo
id I11-R812070
record_format dspace
institution Universidad Nacional de Cuyo
institution_str I-11
repository_str R-8
collection Biblioteca Digital
language Inglés
orig_language_str_mv eng
topic Rhizoctonia solani
Extractos
Ovidia andina
Hongos patógenos
Cumarinas
Flavonoides
Control de enfermedades de plantas
Active fractions
Bioguided fractionation
CH2Cl2 extract
Coumarins and flavonoids characterization
Fungal growth
Hongos patógenos de suelo
Fracciones activas
Extracto CH2Cl2
spellingShingle Rhizoctonia solani
Extractos
Ovidia andina
Hongos patógenos
Cumarinas
Flavonoides
Control de enfermedades de plantas
Active fractions
Bioguided fractionation
CH2Cl2 extract
Coumarins and flavonoids characterization
Fungal growth
Hongos patógenos de suelo
Fracciones activas
Extracto CH2Cl2
Tito Mansilla, Javier
Tarcaya, Verónica P.
Cufre, Ingrid M.
Fabrizio, María C.
Wright, Eduardo R.
Broussalis, Adriana M.
Rivera, Marta C.
Control of Rhizoctonia solani with extracts from Ovidia andina
topic_facet Rhizoctonia solani
Extractos
Ovidia andina
Hongos patógenos
Cumarinas
Flavonoides
Control de enfermedades de plantas
Active fractions
Bioguided fractionation
CH2Cl2 extract
Coumarins and flavonoids characterization
Fungal growth
Hongos patógenos de suelo
Fracciones activas
Extracto CH2Cl2
description New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen. Powdered dried leaves and stems were extracted by maceration with dichloromethane (CH2Cl2), methanol and ethanol. An aqueous extract was obtained by decoction, and the hydrodistillation residual water was the aqueous phase after essences production. A strain of R. solani was cultivated on potato dextrose agar supplemented with the extracts, at 1:100 and 1:1000 v/v. The CH2Cl2 extract at 1:100 reduced colony growth (p< 0.0001) and changed hyphal morphology. Five fractions were obtained from the CH2Cl2 extract in a glass column, and TLC analysis showed the presence of coumarins and flavonoids. The CH2Cl2 extract and fractions were diluted in CH2Cl2 or acetone. The efficiency to reduce pathogen growth of fractions 2f and 5f diluted in CH2Cl2 at 1:100 did not differ from the whole extract (p<0.0001). For an in vivo test, beetroot seed balls were incubated in soil infested with R. solani and treated by immersion in the CH2Cl2 extract at 1:100 for different periods of time. As a result, pathogen colonization was diminished by immersion for 120 s and seed germination was normal (p< 0.0001). Additionally, infested soil was treated with the CH2Cl2 extract, and inoculum concentration estimated using beetroot seed balls as baits diminished (p< 0.0001). These results lead us to conclude that O. andina is a source of antifungal components for crop protection against R. solani.
format article
Artículo
Artículo
publishedVersion
author Tito Mansilla, Javier
Tarcaya, Verónica P.
Cufre, Ingrid M.
Fabrizio, María C.
Wright, Eduardo R.
Broussalis, Adriana M.
Rivera, Marta C.
author_facet Tito Mansilla, Javier
Tarcaya, Verónica P.
Cufre, Ingrid M.
Fabrizio, María C.
Wright, Eduardo R.
Broussalis, Adriana M.
Rivera, Marta C.
author_sort Tito Mansilla, Javier
title Control of Rhizoctonia solani with extracts from Ovidia andina
title_short Control of Rhizoctonia solani with extracts from Ovidia andina
title_full Control of Rhizoctonia solani with extracts from Ovidia andina
title_fullStr Control of Rhizoctonia solani with extracts from Ovidia andina
title_full_unstemmed Control of Rhizoctonia solani with extracts from Ovidia andina
title_sort control of rhizoctonia solani with extracts from ovidia andina
publisher Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
publishDate 2018
url http://bdigital.uncu.edu.ar/12070
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