Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers

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Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect geneti...

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Detalles Bibliográficos
Autores principales:	Carloni, Edgardo, López Colomba, Eliana, Ribotta, Andrea, Quiroga, Mariana, Tommasino, Exequiel, Griffa, Sabrina, Grunberg, Karina
Formato:	article Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 2018
Materias:	Variación genética Genética vegetal Regeneración in vitro Cenchrus ciliaris Marcadores genéticos Cultivo in vitro Apomixis Embriogénesis somática Variación somaclonal Cultivo de anteras Diversidad genética como recurso Somatic embryogenesis Anther culture ISSR Genetic diversity Análisis de variabilidad Buffelgrass
Acceso en línea:	http://bdigital.uncu.edu.ar/11586
Aporte de:	Biblioteca Digital de Universidad Nacional de Cuyo

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institution	Universidad Nacional de Cuyo
institution_str	I-11
repository_str	R-8
collection	Biblioteca Digital
language	Inglés
orig_language_str_mv	eng
topic	Variación genética Genética vegetal Regeneración in vitro Cenchrus ciliaris Marcadores genéticos Cultivo in vitro Apomixis Embriogénesis somática Variación somaclonal Cultivo de anteras Diversidad genética como recurso Apomixis Somatic embryogenesis Anther culture ISSR Genetic diversity Análisis de variabilidad Buffelgrass
spellingShingle	Variación genética Genética vegetal Regeneración in vitro Cenchrus ciliaris Marcadores genéticos Cultivo in vitro Apomixis Embriogénesis somática Variación somaclonal Cultivo de anteras Diversidad genética como recurso Apomixis Somatic embryogenesis Anther culture ISSR Genetic diversity Análisis de variabilidad Buffelgrass Carloni, Edgardo López Colomba, Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
topic_facet	Variación genética Genética vegetal Regeneración in vitro Cenchrus ciliaris Marcadores genéticos Cultivo in vitro Apomixis Embriogénesis somática Variación somaclonal Cultivo de anteras Diversidad genética como recurso Apomixis Somatic embryogenesis Anther culture ISSR Genetic diversity Análisis de variabilidad Buffelgrass
description	Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect genetic variation in in vitro buffelgrass regenerated plants. Six plants regenerated from in vitro anther culture, via somatic embryogenesis were used, as well as the anther donor genotype (RN 51) as control. Of a total of 26 ISSR primers tested, 22 amplified, detecting 12% polymorphism with a divergence between 5 and 24% from RN 51. Amplification products were observed with the primers containing di-, tri- or tetra-nucleotide sequences, with or without additional nucleotides at the 3′ end. The most informative primers were those containing the repetitive sequences GACAn, AGn or GAn. Moreover, the regenerants transplanted at field conditions differed in morphological characteristics among them and with respect to RN 51. This study confirms that ISSR are useful to identify genetic variability in in vitro regenerated buffelgrass plants.
format	article Artículo Artículo publishedVersion
author	Carloni, Edgardo López Colomba, Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina
author_facet	Carloni, Edgardo López Colomba, Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina
author_sort	Carloni, Edgardo
title	Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
title_short	Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
title_full	Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
title_fullStr	Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
title_full_unstemmed	Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
title_sort	analysis of genetic variability in vitro regenerated buffelgrass plants through issr molecular markers
publisher	Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
publishDate	2018
url	http://bdigital.uncu.edu.ar/11586
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Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers

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