Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species

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Trichloris crinita is among the most important native forage grasses in arid regions of America. Despite its importance, molecular resources and sequence data are extremely scarce in this species. In the present study, SSR markers were developed using available DNA sequences from grass taxa phylogen...

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Autores principales: Kozub, Perla Carolina, Barboza, Karina, Cavagnaro, Juan Bruno, Cavagnaro, Pablo Federico
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 2018
Materias:
Trichloris crinita
Gramíneas
Gramíneas forrajeras
Diversidad genética como recurso
Microsatélites
Chloridoideae
Marcadores genéticos
Forage grass
Genetic diversity
Marker transferability
Microsatellites
Acceso en línea:http://bdigital.uncu.edu.ar/10692
Aporte de:
Biblioteca Digital de Universidad Nacional de Cuyo
id I11-R810692
record_format dspace
institution Universidad Nacional de Cuyo
institution_str I-11
repository_str R-8
collection Biblioteca Digital
language Inglés
orig_language_str_mv eng
topic Trichloris crinita
Gramíneas
Gramíneas forrajeras
Diversidad genética como recurso
Microsatélites
Chloridoideae
Marcadores genéticos
Forage grass
Genetic diversity
Marker transferability
Microsatellites
spellingShingle Trichloris crinita
Gramíneas
Gramíneas forrajeras
Diversidad genética como recurso
Microsatélites
Chloridoideae
Marcadores genéticos
Forage grass
Genetic diversity
Marker transferability
Microsatellites
Kozub, Perla Carolina
Barboza, Karina
Cavagnaro, Juan Bruno
Cavagnaro, Pablo Federico
Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species
topic_facet Trichloris crinita
Gramíneas
Gramíneas forrajeras
Diversidad genética como recurso
Microsatélites
Chloridoideae
Marcadores genéticos
Forage grass
Genetic diversity
Marker transferability
Microsatellites
description Trichloris crinita is among the most important native forage grasses in arid regions of America. Despite its importance, molecular resources and sequence data are extremely scarce in this species. In the present study, SSR markers were developed using available DNA sequences from grass taxa phylogenetically-related to Trichloris (Eleusine coracana, Cynodon dactylon and ‘Cynodon dactylon x Cynodon transvaalensis’). Marker transferability was evaluated in a panel of eight T. crinita accessions and five closely-related species. Of the 105 SSR primer pairs evaluated, 16 amplified products of expected size in T. crinita, whereas transferability to other grass species ranged from 12 (in Chloris castilloniana) to 28 SSRs (in Eleusine coracana). Six of the 16 SSR markers successfully transferred to T. crinita (37.5%) were polymorphic, and were further used to assess genetic diversity in eight T. crinita accessions. The analysis revealed a total of 23 SSR alleles (3.83 alleles/locus), allowing the discrimination of all T. crinita accessions, with pair-wise genetic similarities ranging from 0.35 to 0.81 (Jaccard coefficient). Mean (and range) values for observed (Ho) and expected heterozygosity (He) were 0.53 (0.0-1.0) and 0.63 (0.48-0.79), respectively.
format article
Artículo
Artículo
publishedVersion
author Kozub, Perla Carolina
Barboza, Karina
Cavagnaro, Juan Bruno
Cavagnaro, Pablo Federico
author_facet Kozub, Perla Carolina
Barboza, Karina
Cavagnaro, Juan Bruno
Cavagnaro, Pablo Federico
author_sort Kozub, Perla Carolina
title Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species
title_short Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species
title_full Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species
title_fullStr Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species
title_full_unstemmed Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species
title_sort development and characterization of ssr markers for trichloris crinita using sequence data from related grass species
publisher Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
publishDate 2018
url http://bdigital.uncu.edu.ar/10692
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