Benzyladenine concentration, type and dose of carbohydrates in the culture medium for shoot proliferation of Agave americana

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To evaluate the in vitro shoot proliferation of Agave americana var. oaxacensis, calli fragments with two or three adventitious shoots were put in different culture media with pH 5.8 and consistency of gel, containing the MS mineral salts, 100 mg L-1 myo-inositol; benzyladenine (BA) in different con...

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Autores principales: Miguel Luna, Maura Elisama, del Valle, José Raymundo Enríquez, Velasco Velasco, Vicente Arturo, Villegas Aparicio, Yuri, Carrillo Rodríguez, José Cruz
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Ciencias Agrarias-UNCuyo 2022
Materias:
Agave americana
citocinina
jarabe fructosado
propagación in vitro
sacarosa
cytokinin
fructose syrup
in vitro propagation
sucrose
Acceso en línea:https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5632
Aporte de:
Revista de la Facultad de Ciencias Agrarias UNCuyo de Universidad Nacional de Cuyo
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institution Universidad Nacional de Cuyo
institution_str I-11
repository_str R-107
container_title_str Revista de la Facultad de Ciencias Agrarias UNCuyo
language Español
format Artículo revista
topic Agave americana
citocinina
jarabe fructosado
propagación in vitro
sacarosa
Agave americana
cytokinin
fructose syrup
in vitro propagation
sucrose
spellingShingle Agave americana
citocinina
jarabe fructosado
propagación in vitro
sacarosa
Agave americana
cytokinin
fructose syrup
in vitro propagation
sucrose
Miguel Luna, Maura Elisama
del Valle, José Raymundo Enríquez
Velasco Velasco, Vicente Arturo
Villegas Aparicio, Yuri
Carrillo Rodríguez, José Cruz
Benzyladenine concentration, type and dose of carbohydrates in the culture medium for shoot proliferation of Agave americana
topic_facet Agave americana
citocinina
jarabe fructosado
propagación in vitro
sacarosa
Agave americana
cytokinin
fructose syrup
in vitro propagation
sucrose
author Miguel Luna, Maura Elisama
del Valle, José Raymundo Enríquez
Velasco Velasco, Vicente Arturo
Villegas Aparicio, Yuri
Carrillo Rodríguez, José Cruz
author_facet Miguel Luna, Maura Elisama
del Valle, José Raymundo Enríquez
Velasco Velasco, Vicente Arturo
Villegas Aparicio, Yuri
Carrillo Rodríguez, José Cruz
author_sort Miguel Luna, Maura Elisama
title Benzyladenine concentration, type and dose of carbohydrates in the culture medium for shoot proliferation of Agave americana
title_short Benzyladenine concentration, type and dose of carbohydrates in the culture medium for shoot proliferation of Agave americana
title_full Benzyladenine concentration, type and dose of carbohydrates in the culture medium for shoot proliferation of Agave americana
title_fullStr Benzyladenine concentration, type and dose of carbohydrates in the culture medium for shoot proliferation of Agave americana
title_full_unstemmed Benzyladenine concentration, type and dose of carbohydrates in the culture medium for shoot proliferation of Agave americana
title_sort benzyladenine concentration, type and dose of carbohydrates in the culture medium for shoot proliferation of agave americana
description To evaluate the in vitro shoot proliferation of Agave americana var. oaxacensis, calli fragments with two or three adventitious shoots were put in different culture media with pH 5.8 and consistency of gel, containing the MS mineral salts, 100 mg L-1 myo-inositol; benzyladenine (BA) in different concentration (0, 2, 4, 6, 8 and 10 mg L-1), type of carbohydrate (sucrose or fructose syrup) and carbohydrate concentration (20, 30, 40 g L-1). Cultures were incubated for 60 days under white fluorescent light in 16 h light/8 h darkness, temperature 20-28°C. The experiment was established according to a completely randomized design with a factorial arrangement 6x2x3. The sucrose was better carbohydrate than fructose syrup. The explants in media without BA and 20 g L-1 sucrose formed four shoots with 10.8 cm tall, and formed adventitious roots. When the BA and sucrose concentration were increased the explants formed more adventitious shoots, but in the media with 6 mg L-1 BA and 40 g L-1 sucrose the esplants formed up to 21adventitious shoots which had 6.5 cm size. The cytokinin prevented the root formation.
publisher Facultad de Ciencias Agrarias-UNCuyo
publishDate 2022
url https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5632
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first_indexed 2022-06-20T13:54:07Z
last_indexed 2022-06-20T13:54:07Z
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