Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer

In this work, we compared mRNA levels of Hyaluronan (HA) metabolism members and BRCA genes, known to be involved in the tumoral process, between tumor and non-tumor adjacent tissue and its correlation with previously proposed biomarkers (ER, PR, HER2 and KI67) in order to assess their value as a pro...

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Autores principales: Sevic, Ina, Spinelli, Fiorella Mercedes, Vitale, Daiana Luján, Icardi, Antonella, Romano, Lucia, Brandone, Alejandra, Giannoni, Paula, Cristina, Carolina, Bolontrade, Marcela Fabiana, Alaniz, Laura
Otros Autores: https://orcid.org/0000-0002-6070-6078
Formato: Artículo draft
Lenguaje:Inglés
Publicado: MDPI 2020
Materias:
Tumor microenvironment
Hyaluronan
Hyaluronan metabolism
Breast cancer
Colorectal cancer
qPCR
Acceso en línea:http://repositorio.unnoba.edu.ar:8080/xmlui/handle/23601/109
Aporte de:
Re DI Repositorio Digital UNNOBA de Universidad Nacional del Noroeste de la Provincia de Buenos Aires
id I103-R405-23601-109
record_format dspace
institution Universidad Nacional del Noroeste de la Provincia de Buenos Aires
institution_str I-103
repository_str R-405
collection Re DI Repositorio Digital UNNOBA
language Inglés
topic Tumor microenvironment
Hyaluronan
Hyaluronan metabolism
Breast cancer
Colorectal cancer
qPCR
spellingShingle Tumor microenvironment
Hyaluronan
Hyaluronan metabolism
Breast cancer
Colorectal cancer
qPCR
Sevic, Ina
Spinelli, Fiorella Mercedes
Vitale, Daiana Luján
Icardi, Antonella
Romano, Lucia
Brandone, Alejandra
Giannoni, Paula
Cristina, Carolina
Bolontrade, Marcela Fabiana
Alaniz, Laura
Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer
topic_facet Tumor microenvironment
Hyaluronan
Hyaluronan metabolism
Breast cancer
Colorectal cancer
qPCR
description In this work, we compared mRNA levels of Hyaluronan (HA) metabolism members and BRCA genes, known to be involved in the tumoral process, between tumor and non-tumor adjacent tissue and its correlation with previously proposed biomarkers (ER, PR, HER2 and KI67) in order to assess their value as a progression biomarkers. We show alteration in HA metabolism in colorectal but not breast cancer. However, we found a decrease in Hyaluronidase 1 HYAL1 levels in the breast but not colorectal cancer. We also show lower HA levels in tumor compared with normal tissue that could indicate a possible influence of tumor on its surrounding "normal" tissue. In both breast and colorectal cancer, CD44 and BRCA2 showed a strong positive correlation. Besides, our results show first indicators that qPCR of the analyzed genes could be used as an easy and low cost procedure for the evaluation of molecular markers we propose here
author2 https://orcid.org/0000-0002-6070-6078
author_facet https://orcid.org/0000-0002-6070-6078
Sevic, Ina
Spinelli, Fiorella Mercedes
Vitale, Daiana Luján
Icardi, Antonella
Romano, Lucia
Brandone, Alejandra
Giannoni, Paula
Cristina, Carolina
Bolontrade, Marcela Fabiana
Alaniz, Laura
format Artículo
Artículo
draft
Artículo
Artículo
draft
Artículo
Artículo
draft
author Sevic, Ina
Spinelli, Fiorella Mercedes
Vitale, Daiana Luján
Icardi, Antonella
Romano, Lucia
Brandone, Alejandra
Giannoni, Paula
Cristina, Carolina
Bolontrade, Marcela Fabiana
Alaniz, Laura
author_sort Sevic, Ina
title Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer
title_short Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer
title_full Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer
title_fullStr Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer
title_full_unstemmed Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer
title_sort hyaluronan metabolism is associated with dna repair genes in breast and colorectal cancer
publisher MDPI
publishDate 2020
url http://repositorio.unnoba.edu.ar:8080/xmlui/handle/23601/109
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spelling I103-R405-23601-1092025-10-21T21:00:36Z Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer Screening of Potential Progression Markers Using qPCR Sevic, Ina Spinelli, Fiorella Mercedes Vitale, Daiana Luján Icardi, Antonella Romano, Lucia Brandone, Alejandra Giannoni, Paula Cristina, Carolina Bolontrade, Marcela Fabiana Alaniz, Laura https://orcid.org/0000-0002-6070-6078 https://orcid.org/0000-0002-4920-5096 https://orcid.org/0000-0002-8664-5541 https://orcid.org/0000-0001-8451-7055 https://orcid.org/0000-0001-5593-9101 https://orcid.org/0000-0003-0459-6721 Tumor microenvironment Hyaluronan Hyaluronan metabolism Breast cancer Colorectal cancer qPCR In this work, we compared mRNA levels of Hyaluronan (HA) metabolism members and BRCA genes, known to be involved in the tumoral process, between tumor and non-tumor adjacent tissue and its correlation with previously proposed biomarkers (ER, PR, HER2 and KI67) in order to assess their value as a progression biomarkers. We show alteration in HA metabolism in colorectal but not breast cancer. However, we found a decrease in Hyaluronidase 1 HYAL1 levels in the breast but not colorectal cancer. We also show lower HA levels in tumor compared with normal tissue that could indicate a possible influence of tumor on its surrounding "normal" tissue. In both breast and colorectal cancer, CD44 and BRCA2 showed a strong positive correlation. Besides, our results show first indicators that qPCR of the analyzed genes could be used as an easy and low cost procedure for the evaluation of molecular markers we propose here Fil: Ina, Sevic. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Spinelli, Fiorella Mercedes. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Vitale, Daiana Lujan. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Icardi, Antonella. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Brandone, Alejandra, Hospital Interzonal General de Agudos Dr. Abraham F. Piñeyro; Argentina. Fil: Giannoni, Paula. Clínica Centro; Argentina. Fil: Romano, Lucia. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicadas (CIBA); Argentina. Fil: Cristina, Carolina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicada. Laboratorio de Fisiopatología de la Hipófisis; Argentina. Fil: Bolontrade, Marcela Fabiana. Hospital Italiano. Instituto Universitario del Hospital Italiano-Instituto de Medicina Traslacional e Ingeniería Biomédica (IMTIB)-CONICET-Hospital Italiano. Buenos Aires (HIBA); Argentina. Fil: Alaniz, Laura. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Con referato 2020-09-25T15:43:16Z 2020-09-25T15:43:16Z 2020-06-29 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/draft info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/draft info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/draft Sevic, I., Spinelli, F. M., Vitale, D. L., Icardi, A., Romano, L., Brandone, A., Giannoni, P., Cristina, C., Bolontrade, M. F., y Alaniz, L. (2020). Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer. Biomedicines. http://repositorio.unnoba.edu.ar:8080/xmlui/handle/23601/109 2227-9059 http://repositorio.unnoba.edu.ar:8080/xmlui/handle/23601/109 eng info:eu-repo/grantAgreement/ Fundación ROEMMERS, Subsidio para Investigación Médica 2017 info:eu-repo/grantAgreement/UNNOBA/SIB/2019-0561 https://doi.org/10.3390/biomedicines8070183 info:eu-repo/semantics/closedAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf MDPI Biomedicines

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