Production of antiserum against potato virus X (Solanum tuberosum L.) and its incorporation into ELISA detection services

The potato virus X was purified fromNicotiana glutinosa L. tissues, clarifying the plantsap by low speed centrifugation cycles, precipitations with polyethyleneglycol, sucrose cushion and sucrose density gradient ultracentrifugation. Rabbits were immunized with the purified preparations and a titer...

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Autores principales: Weller, F. M., Rodríguez, F. I., Medina, G. I., Muñoz, J. O.
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Ciencias Agropecuarias 1990
Materias:
Potato virus X
Antiserum
ELISA
Nicotiana glutinosa
virus X de la papa
antisuero
Acceso en línea:https://revistas.unc.edu.ar/index.php/agris/article/view/939
Aporte de:
AgriScientia de Universidad Nacional de Córdoba
id I10-R352-article-939
record_format ojs
spelling I10-R352-article-9392025-06-03T15:54:00Z Production of antiserum against potato virus X (Solanum tuberosum L.) and its incorporation into ELISA detection services Producción de antisuero contra el virus X de la papa (Solanum tuberosum L.) y su incorporación a los servicios de detección mediante ELISA Weller, F. M. Rodríguez, F. I. Medina, G. I. Muñoz, J. O. Potato virus X Antiserum ELISA Nicotiana glutinosa virus X de la papa antisuero ELISA Nicotiana glutinosa The potato virus X was purified fromNicotiana glutinosa L. tissues, clarifying the plantsap by low speed centrifugation cycles, precipitations with polyethyleneglycol, sucrose cushion and sucrose density gradient ultracentrifugation. Rabbits were immunized with the purified preparations and a titer of 1/128 in microproecipitation tests was obtained. The immunóglobulin G was purified, and conjugated with alkaline phosphatase to be used in ELISA tests. The immunoglobulin G and conjugate are at present being used by the departmentin virus detection in potatoes. Se purificó el virus X de la papa a partir de Nicotiana glutinosa L., clarificando el jugo vegetal por medio de ciclos de centrifugación a baja velocidad, precipitación con polietilenglicol , colchón de sacarosa y ultracentrifugación en gradiente de densidad de sacarosa. Con los purificados se inmunizaron conejos obteniéndose un antisuero con título 1/128 en pruebas de micro precipitación . Se purificó la gammaglobulina, parte de la cual fue conjugada con fosfatasa alcalina para utilizarla en pruebas ELISA. La gamaglobulina y conjugados obtenidos han sido incorporados al servicio de detección de virus de papa. Facultad de Ciencias Agropecuarias 1990-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/939 10.31047/1668.298x.v7.n1.939 AgriScientia; Vol. 7 (1990); 27-30 AgriScientia; Vol. 7 (1990); 27-30 1668-298X 10.31047/1668.298x.v7.n1 spa https://revistas.unc.edu.ar/index.php/agris/article/view/939/873 Derechos de autor 1990 F M. Weller, F. Rodríguez, G. I. Medina, J. O. Muñoz https://creativecommons.org/licenses/by-sa/4.0
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-352
container_title_str AgriScientia
language Español
format Artículo revista
topic Potato virus X
Antiserum
ELISA
Nicotiana glutinosa
virus X de la papa
antisuero
ELISA
Nicotiana glutinosa
spellingShingle Potato virus X
Antiserum
ELISA
Nicotiana glutinosa
virus X de la papa
antisuero
ELISA
Nicotiana glutinosa
Weller, F. M.
Rodríguez, F. I.
Medina, G. I.
Muñoz, J. O.
Production of antiserum against potato virus X (Solanum tuberosum L.) and its incorporation into ELISA detection services
topic_facet Potato virus X
Antiserum
ELISA
Nicotiana glutinosa
virus X de la papa
antisuero
ELISA
Nicotiana glutinosa
author Weller, F. M.
Rodríguez, F. I.
Medina, G. I.
Muñoz, J. O.
author_facet Weller, F. M.
Rodríguez, F. I.
Medina, G. I.
Muñoz, J. O.
author_sort Weller, F. M.
title Production of antiserum against potato virus X (Solanum tuberosum L.) and its incorporation into ELISA detection services
title_short Production of antiserum against potato virus X (Solanum tuberosum L.) and its incorporation into ELISA detection services
title_full Production of antiserum against potato virus X (Solanum tuberosum L.) and its incorporation into ELISA detection services
title_fullStr Production of antiserum against potato virus X (Solanum tuberosum L.) and its incorporation into ELISA detection services
title_full_unstemmed Production of antiserum against potato virus X (Solanum tuberosum L.) and its incorporation into ELISA detection services
title_sort production of antiserum against potato virus x (solanum tuberosum l.) and its incorporation into elisa detection services
description The potato virus X was purified fromNicotiana glutinosa L. tissues, clarifying the plantsap by low speed centrifugation cycles, precipitations with polyethyleneglycol, sucrose cushion and sucrose density gradient ultracentrifugation. Rabbits were immunized with the purified preparations and a titer of 1/128 in microproecipitation tests was obtained. The immunóglobulin G was purified, and conjugated with alkaline phosphatase to be used in ELISA tests. The immunoglobulin G and conjugate are at present being used by the departmentin virus detection in potatoes.
publisher Facultad de Ciencias Agropecuarias
publishDate 1990
url https://revistas.unc.edu.ar/index.php/agris/article/view/939
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last_indexed 2025-06-10T05:11:11Z
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