Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography
Peanut (Arachis hypogaea L.), a major source of oil, is the second most widely cultivated legume crop worldwide after soybean. Argentina is the largest producer of confectionery peanuts in Latin America and the seventh in the world. This legume has bioactive compounds of nutritional value, phytoster...
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Facultad de Ciencias Agropecuarias
2025
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/agris/article/view/44246 |
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I10-R352-article-442462025-07-21T15:12:47Z Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography Implementación de un método para la identificación y cuantificación de fitoesteroles en semillas de maní (Arachis hypogaea L.) por cromatografía gaseosa Aquilano, Claudio Gabriel Percibaldi, Nora Mabel Martínez, María José Aquilano, Claudio Gabriel sterols cholesterol beta-sitosterol stigmasterol mass spectrometry esteroles colesterol beta-sitosterol estigmasterol espectrometría de masas Peanut (Arachis hypogaea L.), a major source of oil, is the second most widely cultivated legume crop worldwide after soybean. Argentina is the largest producer of confectionery peanuts in Latin America and the seventh in the world. This legume has bioactive compounds of nutritional value, phytosterols among the most important ones. Phytosterols protect against cancer and lower cholesterol rates. The objective of this work was to adjust and refine a method to identify and quantify phytosterol content in peanut seeds by gas chromatography. The method was found to be accurate, as demonstrated through repeatability and reproducibility tests, and expressed by the coefficients of variation. No significant differences were found between the results obtained and the reference values using a confidence level of 95 % and 5 degrees of freedom. The results obtained in this work presented 44.31 mg of beta-sitosterol and 3.39 mg of stigmasterol per 100 g of roasted peanuts, in agreement with the values reported in the literature. El maní (Arachis hipogaea L.), una importante fuente de aceite, es la segunda leguminosa más cultivada en todo el mundo después de la soja. Argentina es el mayor productor de maní confitero de América Latina y el séptimo del mundo. Esta leguminosa posee compuestos bioactivos y los fitoesteroles son uno de los más importantes. Los fitoesteroles protegen contra el cáncer y reducen el colesterol. El objetivo de este trabajo fue ajustar y mejorar un método para identificar y cuantificar el contenido de fitoesteroles en semillas de maní mediante cromatografía de gases. Se concluyó que el método era preciso, como lo demuestran las pruebas de repetibilidad y reproducibilidad expresados por los coeficientes de variación. No se encontraron diferencias significativas entre los resultados obtenidos y los valores de referencia utilizando un nivel de confianza del 95 % y 5 grados de libertad. Los resultados obtenidos en este trabajo correspondiente a 44,31 mg de beta-sitosterol y 3,39 mg de estigmasterol por 100 g de maní tostado, concuerdan con los informados en la literatura. Facultad de Ciencias Agropecuarias 2025-01-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/44246 10.31047/1668.298x.v41.n2.44246 AgriScientia; ##issue.vol## 41 ##issue.no## 2 (2024); 19-26 AgriScientia; Vol. 41 Núm. 2 (2024); 19-26 1668-298X 10.31047/1668.298x.v41.n2 eng https://revistas.unc.edu.ar/index.php/agris/article/view/44246/48393 Derechos de autor 2025 Claudio Gabriel Aquilano, Nora Mabel Percibaldi, María José Martínez https://creativecommons.org/licenses/by-sa/4.0 |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-352 |
| container_title_str |
AgriScientia |
| language |
Inglés |
| format |
Artículo revista |
| topic |
sterols cholesterol beta-sitosterol stigmasterol mass spectrometry esteroles colesterol beta-sitosterol estigmasterol espectrometría de masas |
| spellingShingle |
sterols cholesterol beta-sitosterol stigmasterol mass spectrometry esteroles colesterol beta-sitosterol estigmasterol espectrometría de masas Aquilano, Claudio Gabriel Percibaldi, Nora Mabel Martínez, María José Aquilano, Claudio Gabriel Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| topic_facet |
sterols cholesterol beta-sitosterol stigmasterol mass spectrometry esteroles colesterol beta-sitosterol estigmasterol espectrometría de masas |
| author |
Aquilano, Claudio Gabriel Percibaldi, Nora Mabel Martínez, María José Aquilano, Claudio Gabriel |
| author_facet |
Aquilano, Claudio Gabriel Percibaldi, Nora Mabel Martínez, María José Aquilano, Claudio Gabriel |
| author_sort |
Aquilano, Claudio Gabriel |
| title |
Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_short |
Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_full |
Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_fullStr |
Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_full_unstemmed |
Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_sort |
implementation of a method for the identification and quantification of phytosterols in peanut (arachis hypogaea l.) by gas chromatography |
| description |
Peanut (Arachis hypogaea L.), a major source of oil, is the second most widely cultivated legume crop worldwide after soybean. Argentina is the largest producer of confectionery peanuts in Latin America and the seventh in the world. This legume has bioactive compounds of nutritional value, phytosterols among the most important ones. Phytosterols protect against cancer and lower cholesterol rates. The objective of this work was to adjust and refine a method to identify and quantify phytosterol content in peanut seeds by gas chromatography. The method was found to be accurate, as demonstrated through repeatability and reproducibility tests, and expressed by the coefficients of variation. No significant differences were found between the results obtained and the reference values using a confidence level of 95 % and 5 degrees of freedom. The results obtained in this work presented 44.31 mg of beta-sitosterol and 3.39 mg of stigmasterol per 100 g of roasted peanuts, in agreement with the values reported in the literature. |
| publisher |
Facultad de Ciencias Agropecuarias |
| publishDate |
2025 |
| url |
https://revistas.unc.edu.ar/index.php/agris/article/view/44246 |
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2025-02-05T22:12:25Z |
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