Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina

Orthotospoviruses cause serious economic damage, being a major limitation for production and quality in different crops. The objectives of this work were to detect the presence of orthotospovirus that are infecting cucurbit crops in the country and analyze its phylogeny. Samples were taken in waterm...

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Autores principales: Pozzi, Elizabeth, Luciani, C. E., Giovanni Celli, M., Conci, V. C., Perotto, M. C.
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Ciencias Agropecuarias 2021
Materias:
GRSV
watermelon
RT-PCR
ELISA
sandía
Acceso en línea:https://revistas.unc.edu.ar/index.php/agris/article/view/26732
Aporte de:
AgriScientia de Universidad Nacional de Córdoba
id I10-R352-article-26732
record_format ojs
spelling I10-R352-article-267322024-06-18T19:25:38Z Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina Citrullus lanatus: un nuevo hospedante natural del Groundnut ringspot orthotospovirus en Argentina Pozzi, Elizabeth Luciani, C. E. Giovanni Celli, M. Conci, V. C. Perotto, M. C. GRSV watermelon RT-PCR ELISA GRSV sandía RT-PCR ELISA Orthotospoviruses cause serious economic damage, being a major limitation for production and quality in different crops. The objectives of this work were to detect the presence of orthotospovirus that are infecting cucurbit crops in the country and analyze its phylogeny. Samples were taken in watermelon (Citrullus lanatus) production lots during 2017/2018 in Santiago del Estero province. The results of this work showed in the watermelon samples showed the presence of characteristic virions of the Orthotospovirus genus, and were positive to serological and molecular tests with specific reagents (DAS-ELISA and RTPCR) confirming the presence of groundnut ringspot orthotospovirus (GRSV). Genomic fragments were amplified, sequenced and deposited in GenBank, access nº MK680832 and MK680833. Phylogenetic analysis of N-protein nucleotide partial sequences showed that GRSV isolates from Argentina (watermelon host) were grouped into a new group with high bootstrap value. These sequences were placed in a cluster formed by sequences from the American continent that were separated from those that conformed the group of sequences of African origin. This work is the first report of the presence of the GRSV in watermelon (Citrullus lanatus) cultivation in Argentina. Los orthotospovirus causan graves daños económicos, siendo una limitación importante para la producción y la calidad en distintos cultivos. Los objetivos de este trabajo fueron identificar el agente causal de los síntomas observados en sandía en lotes de producción de Santiago de Estero y analizar su filogenia. Se realizaron muestreos en lotes de producción de sandía (Citrullus lanatus) durante la campaña 2017/2018 en la provincia de Santiago del Estero. Los resultados de este trabajo mostraron en las muestras de sandía la presencia de viriones característicos del género Orthotospovirus, además fueron positivas a pruebas serológicas y moleculares con reactivos específicos (DAS-ELISA y RTPCR) confirmando la presencia del Groundnut ringspot orthotospovirus (GRSV). Los fragmentos genómicos fueron amplificados, secuenciados y depositados en GenBank, acceso Nº MK680832 y MK680833. El análisis filogenético de las secuencias parciales de nucleótidos de la proteína N mostró que los aislamientos de GRSV provenientes de Argentina (hospedante sandía) fueron agrupados con alto valor de confianza. Estas secuencias se ubicaron en un clúster formado por secuencias del continente americano que se separaron de las de origen africano. Este trabajo constituye el primer reporte de la presencia del GRSV en cultivo de sandía (Citrullus lanatus) en Argentina. Facultad de Ciencias Agropecuarias 2021-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion text/html application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/26732 10.31047/1668.298x.v38.n1.26732 AgriScientia; Vol. 38 No. 1 (2021): ; 71-78 AgriScientia; Vol. 38 Núm. 1 (2021): ; 71-78 1668-298X 10.31047/1668.298x.v38.n1 spa https://revistas.unc.edu.ar/index.php/agris/article/view/26732/34964 https://revistas.unc.edu.ar/index.php/agris/article/view/26732/34011 Derechos de autor 2021 Elizabeth Pozzi, C. E. Luciani, M. Giovanni Celli, V. C. Conci, M. C. Perotto https://creativecommons.org/licenses/by-sa/4.0
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-352
container_title_str AgriScientia
language Español
format Artículo revista
topic GRSV
watermelon
RT-PCR
ELISA
GRSV
sandía
RT-PCR
ELISA
spellingShingle GRSV
watermelon
RT-PCR
ELISA
GRSV
sandía
RT-PCR
ELISA
Pozzi, Elizabeth
Luciani, C. E.
Giovanni Celli, M.
Conci, V. C.
Perotto, M. C.
Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina
topic_facet GRSV
watermelon
RT-PCR
ELISA
GRSV
sandía
RT-PCR
ELISA
author Pozzi, Elizabeth
Luciani, C. E.
Giovanni Celli, M.
Conci, V. C.
Perotto, M. C.
author_facet Pozzi, Elizabeth
Luciani, C. E.
Giovanni Celli, M.
Conci, V. C.
Perotto, M. C.
author_sort Pozzi, Elizabeth
title Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina
title_short Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina
title_full Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina
title_fullStr Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina
title_full_unstemmed Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina
title_sort citrullus lanatus: a new natural host of groundnut ringspot orthotospovirus in argentina
description Orthotospoviruses cause serious economic damage, being a major limitation for production and quality in different crops. The objectives of this work were to detect the presence of orthotospovirus that are infecting cucurbit crops in the country and analyze its phylogeny. Samples were taken in watermelon (Citrullus lanatus) production lots during 2017/2018 in Santiago del Estero province. The results of this work showed in the watermelon samples showed the presence of characteristic virions of the Orthotospovirus genus, and were positive to serological and molecular tests with specific reagents (DAS-ELISA and RTPCR) confirming the presence of groundnut ringspot orthotospovirus (GRSV). Genomic fragments were amplified, sequenced and deposited in GenBank, access nº MK680832 and MK680833. Phylogenetic analysis of N-protein nucleotide partial sequences showed that GRSV isolates from Argentina (watermelon host) were grouped into a new group with high bootstrap value. These sequences were placed in a cluster formed by sequences from the American continent that were separated from those that conformed the group of sequences of African origin. This work is the first report of the presence of the GRSV in watermelon (Citrullus lanatus) cultivation in Argentina.
publisher Facultad de Ciencias Agropecuarias
publishDate 2021
url https://revistas.unc.edu.ar/index.php/agris/article/view/26732
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first_indexed 2024-09-03T22:16:26Z
last_indexed 2024-09-03T22:16:26Z
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