Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes

Twenty-one cultivars of Vitis vinifera L. and three rootstocks were chosen to study their biochemical characterization based on the electrophoretic separation of isoenzyme systems obtained from root tissue extracts. Acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloac...

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Autores principales: Altube, H. A., Cabello, F., Ortiz, J. M.
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Ciencias Agropecuarias 1992
Materias:
ampelography
chemotaxonomy
variety of vine
rootstock
root
analysis
enzyme
protein
ampelografía
quimiotaxonomía
variedades de vid
portaingertos
raíz
análisis
enzimas
proteínas
Acceso en línea:https://revistas.unc.edu.ar/index.php/agris/article/view/2369
Aporte de:
AgriScientia de Universidad Nacional de Córdoba
id I10-R352-article-2369
record_format ojs
spelling I10-R352-article-23692025-06-24T14:24:16Z Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes Caracterización de variedades y portainjertos de vid (Vitis vinifera L.) mediante isoenzimas de las raíces Altube, H. A. Cabello, F. Ortiz, J. M. ampelography chemotaxonomy variety of vine rootstock root analysis enzyme protein ampelografía quimiotaxonomía variedades de vid portaingertos raíz análisis enzimas proteínas Twenty-one cultivars of Vitis vinifera L. and three rootstocks were chosen to study their biochemical characterization based on the electrophoretic separation of isoenzyme systems obtained from root tissue extracts. Acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloacetate transaminases (GOT), malate dehydrogenases (MDH), and peroxidases (PER) were separated using polyacrylamide gels and later stained with specific stains for the various isoenzyme systems. ACPH, CO, EST, and MDH were found to be the most adequate systems for characterization. The results of the study of the different systems and the grouping of the cultivars are presented. The use of isoenzymatic techniques for the characterization of Vitis genotypes is suggested. Se eligieron 21 cultivares de Vitis vinifera L. y tres portainjertos para la caracterización bioquímica basada en la separación electroforética de las isoenzimas de extractos de raíz. Los sistemas isoenzimáticos estudiados fueron: fosfatasas ácidas (ACPH), esterasas (EST), catecol oxidasas (CO), glutamato oxalacetato transaminasas (GOT), malato deshidrogenasas (MDH) y peroxidasas (PER). La separación de los extractos de raíz se llevó a cabo en gel de poliacrilamida, con posterior tinción específica para los distintos sistemas enzimáticos. ACPH, CO, EST y MDH fueron los sistemas más adecuados para la caracterización. Se presentan los resultados de los diferentes sistemas y la agrupación de cultivares. Se recomienda el uso de técnicas isoenzimáticas en los estudios de caracterización del género Vitis. Facultad de Ciencias Agropecuarias 1992-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/2369 10.31047/1668.298x.v9.n2.2369 AgriScientia; ##issue.vol## 9 ##issue.no## 2 (1992); 21-29 AgriScientia; Vol. 9 Núm. 2 (1992); 21-29 1668-298X 10.31047/1668.298x.v9.n2 spa https://revistas.unc.edu.ar/index.php/agris/article/view/2369/1313 Derechos de autor 1992 H. A. Altube, F. Cabello, J. M. Ortiz https://creativecommons.org/licenses/by-sa/4.0
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-352
container_title_str AgriScientia
language Español
format Artículo revista
topic ampelography
chemotaxonomy
variety of vine
rootstock
root
analysis
enzyme
protein
ampelografía
quimiotaxonomía
variedades de vid
portaingertos
raíz
análisis
enzimas
proteínas
spellingShingle ampelography
chemotaxonomy
variety of vine
rootstock
root
analysis
enzyme
protein
ampelografía
quimiotaxonomía
variedades de vid
portaingertos
raíz
análisis
enzimas
proteínas
Altube, H. A.
Cabello, F.
Ortiz, J. M.
Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes
topic_facet ampelography
chemotaxonomy
variety of vine
rootstock
root
analysis
enzyme
protein
ampelografía
quimiotaxonomía
variedades de vid
portaingertos
raíz
análisis
enzimas
proteínas
author Altube, H. A.
Cabello, F.
Ortiz, J. M.
author_facet Altube, H. A.
Cabello, F.
Ortiz, J. M.
author_sort Altube, H. A.
title Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes
title_short Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes
title_full Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes
title_fullStr Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes
title_full_unstemmed Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes
title_sort characterization of grapevine (vitis vinifera l.) varieties and rootstocks by root isoenzymes
description Twenty-one cultivars of Vitis vinifera L. and three rootstocks were chosen to study their biochemical characterization based on the electrophoretic separation of isoenzyme systems obtained from root tissue extracts. Acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloacetate transaminases (GOT), malate dehydrogenases (MDH), and peroxidases (PER) were separated using polyacrylamide gels and later stained with specific stains for the various isoenzyme systems. ACPH, CO, EST, and MDH were found to be the most adequate systems for characterization. The results of the study of the different systems and the grouping of the cultivars are presented. The use of isoenzymatic techniques for the characterization of Vitis genotypes is suggested.
publisher Facultad de Ciencias Agropecuarias
publishDate 1992
url https://revistas.unc.edu.ar/index.php/agris/article/view/2369
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AT cabellof characterizationofgrapevinevitisviniferalvarietiesandrootstocksbyrootisoenzymes
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AT altubeha caracterizaciondevariedadesyportainjertosdevidvitisviniferalmedianteisoenzimasdelasraices
AT cabellof caracterizaciondevariedadesyportainjertosdevidvitisviniferalmedianteisoenzimasdelasraices
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first_indexed 2024-09-03T22:15:02Z
last_indexed 2025-08-10T05:28:54Z
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