Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation

Micropropagation of Actinidia chinensis Planch. cv. Hayward (kiwi) plants was possible by the culture of resting buds excised in August. The initiation of the explant growth and the proliferation of the axillaries was achieved by the use of the MS (1962) salt formulation, organic compounds of Standa...

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Autores principales: Radice, S., Caso, O. H.
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Ciencias Agropecuarias 1991
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Acceso en línea:https://revistas.unc.edu.ar/index.php/agris/article/view/2249
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Sumario:Micropropagation of Actinidia chinensis Planch. cv. Hayward (kiwi) plants was possible by the culture of resting buds excised in August. The initiation of the explant growth and the proliferation of the axillaries was achieved by the use of the MS (1962) salt formulation, organic compounds of Standardi (1983), 20 g. L⁻¹ sucrose and (mg. L⁻¹) 1 BA and 0,1 GA₃. After 30 days, a 4–5 multiplication rate was obtained. Root formation was possible when the macronutrient composition of MS was half reduced, with the same sucrose concentration and the addition of IBA and PPZ, both at 1 mg. L⁻¹. A rooting percentage of 95 was obtained after 30 days. The presented protocol resulted in the production of near 1000 hardened plantlets.