Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation

Micropropagation of Actinidia chinensis Planch. cv. Hayward (kiwi) plants was possible by the culture of resting buds excised in August. The initiation of the explant growth and the proliferation of the axillaries was achieved by the use of the MS (1962) salt formulation, organic compounds of Standa...

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Autores principales: Radice, S., Caso, O. H.
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Ciencias Agropecuarias 1991
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Acceso en línea:https://revistas.unc.edu.ar/index.php/agris/article/view/2249
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spelling I10-R352-article-22492025-06-17T14:39:07Z Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation Micropropagación de Actinidia chinensis Planch. (Kiwi). Optimización del medio de cultivo para la propagación clonal en gran escala Radice, S. Caso, O. H. actinidia chinensis micropropagation kiwifruit Actinidia chinensis micropropagación Kiwi Micropropagation of Actinidia chinensis Planch. cv. Hayward (kiwi) plants was possible by the culture of resting buds excised in August. The initiation of the explant growth and the proliferation of the axillaries was achieved by the use of the MS (1962) salt formulation, organic compounds of Standardi (1983), 20 g. L⁻¹ sucrose and (mg. L⁻¹) 1 BA and 0,1 GA₃. After 30 days, a 4–5 multiplication rate was obtained. Root formation was possible when the macronutrient composition of MS was half reduced, with the same sucrose concentration and the addition of IBA and PPZ, both at 1 mg. L⁻¹. A rooting percentage of 95 was obtained after 30 days. The presented protocol resulted in the production of near 1000 hardened plantlets. La micropropagación en escala comercial de kiwi (Actinidia chinensis Planch.) cv. Hayward fue posible a partir de la siembra de ápices extraídos de yemas dormidas. El establecimiento y crecimiento del explanto se logró con el cultivo en medio MS (1962), los compuestos orgánicos de Standards (1983), 209 L⁻¹ sacarosa, sin auxinas y con el agregado de 1 mg. L⁻¹ de BA y 0,1 mg. L⁻¹ de GA₃. El empleo de ANA determinó la formación de un callo basal y el crecimiento anormal de la yema. La brotación de las yemas axilares se obtuvo con un medio compuesto por las sales de MS, los componentes orgánicos de Standards (1983) e idéntica composición de reguladores del crecimiento. En esta forma se obtuvieron 4-5 brotes por explanto cada 30 días, en condiciones de iniciar el enraizamiento. Esto se logró con la reducción al 50% de los macronutrientes de MS, IBA y PPZ, éstos últimos a razón de 1 mg. L⁻¹. El porcentaje de enraizamiento llegó al 95% al cabo de 30 días, con la producción de cerca de 1000 plantitas rusticadas. Facultad de Ciencias Agropecuarias 1991-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/2249 10.31047/1668.298x.v8.n0.2249 AgriScientia; ##issue.vol## 8 (1991); 55-60 AgriScientia; Vol. 8 (1991); 55-60 1668-298X 10.31047/1668.298x.v8.n0 spa https://revistas.unc.edu.ar/index.php/agris/article/view/2249/1198 Derechos de autor 1991 S. Radice, O. H. Caso https://creativecommons.org/licenses/by-sa/4.0
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-352
container_title_str AgriScientia
language Español
format Artículo revista
topic actinidia chinensis
micropropagation
kiwifruit
Actinidia chinensis
micropropagación
Kiwi
spellingShingle actinidia chinensis
micropropagation
kiwifruit
Actinidia chinensis
micropropagación
Kiwi
Radice, S.
Caso, O. H.
Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation
topic_facet actinidia chinensis
micropropagation
kiwifruit
Actinidia chinensis
micropropagación
Kiwi
author Radice, S.
Caso, O. H.
author_facet Radice, S.
Caso, O. H.
author_sort Radice, S.
title Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation
title_short Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation
title_full Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation
title_fullStr Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation
title_full_unstemmed Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation
title_sort micropropagation of actinidia chinensis planch (kiwi). optimization of the culture medium for large-scale clonal propagation
description Micropropagation of Actinidia chinensis Planch. cv. Hayward (kiwi) plants was possible by the culture of resting buds excised in August. The initiation of the explant growth and the proliferation of the axillaries was achieved by the use of the MS (1962) salt formulation, organic compounds of Standardi (1983), 20 g. L⁻¹ sucrose and (mg. L⁻¹) 1 BA and 0,1 GA₃. After 30 days, a 4–5 multiplication rate was obtained. Root formation was possible when the macronutrient composition of MS was half reduced, with the same sucrose concentration and the addition of IBA and PPZ, both at 1 mg. L⁻¹. A rooting percentage of 95 was obtained after 30 days. The presented protocol resulted in the production of near 1000 hardened plantlets.
publisher Facultad de Ciencias Agropecuarias
publishDate 1991
url https://revistas.unc.edu.ar/index.php/agris/article/view/2249
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first_indexed 2024-09-03T21:44:45Z
last_indexed 2025-08-10T05:28:51Z
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