Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study

Natural products have been resurgent in recent years, intending to palliate oral ailments. For this reason, Tea Tree Oil (TTO) is proposed as the active ingredient of a gel for dental use, which can be used for periodontal disease. In this research, we compared TTO’s efficacy in contrast with chl...

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Autores principales:	Díaz Basurto, Diana D, Macín Cabrera, Susana A, Noguez Méndez, Norma A, Rubio Martínez, Alejandro, Juárez Sandoval, José
Formato:	Artículo revista
Lenguaje:	Inglés
Publicado:	Facultad de Odontología 2025
Materias:	tea tree oil periodontal disease periodontal pathogenic miroorganisms antimicrobial chlorexidine aceite de árbol de té enfermedad periodontal microorganismos patógenos periodontales antimicrobiano clorhexidina
Acceso en línea:	https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/48695
Aporte de:	Revista de la Facultad de Odontología de Universidad Nacional de Córdoba

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container_title_str	Revista de la Facultad de Odontología
language	Inglés
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topic	tea tree oil periodontal disease periodontal pathogenic miroorganisms antimicrobial chlorexidine aceite de árbol de té enfermedad periodontal microorganismos patógenos periodontales antimicrobiano clorhexidina
spellingShingle	tea tree oil periodontal disease periodontal pathogenic miroorganisms antimicrobial chlorexidine aceite de árbol de té enfermedad periodontal microorganismos patógenos periodontales antimicrobiano clorhexidina Díaz Basurto, Diana D Macín Cabrera, Susana A Noguez Méndez, Norma A Rubio Martínez, Alejandro Juárez Sandoval, José Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study
topic_facet	tea tree oil periodontal disease periodontal pathogenic miroorganisms antimicrobial chlorexidine aceite de árbol de té enfermedad periodontal microorganismos patógenos periodontales antimicrobiano clorhexidina
author	Díaz Basurto, Diana D Macín Cabrera, Susana A Noguez Méndez, Norma A Rubio Martínez, Alejandro Juárez Sandoval, José
author_facet	Díaz Basurto, Diana D Macín Cabrera, Susana A Noguez Méndez, Norma A Rubio Martínez, Alejandro Juárez Sandoval, José
author_sort	Díaz Basurto, Diana D
title	Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study
title_short	Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study
title_full	Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study
title_fullStr	Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study
title_full_unstemmed	Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study
title_sort	evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. in vitro study
description	Natural products have been resurgent in recent years, intending to palliate oral ailments. For this reason, Tea Tree Oil (TTO) is proposed as the active ingredient of a gel for dental use, which can be used for periodontal disease. In this research, we compared TTO’s efficacy in contrast with chlorhexidine, against Porphyromonas gingivalis (P. gingivalis), Prevotella intermedia (P. intermedia), Fusobacterium nucleatum (F. nucleatum) and Parviromonas micra (P. micra). Dental plaque biofilm samples were collected from 30 subjects diagnosed with periodontal disease. Samples were subsequently cultivated, isolated, and verified. Then, the Kirby-Bauer method was used to evaluate the antimicrobial activity (n=360). The statistical test results indicated no statistically significant differences from chlorhexidine (CHX) for three bacteria: P. intermedia, F. nucleatum, and P. micra. In contrast, for P. gingivalis, there is a significant difference (p<0.001). However, this difference could be reduced if the concentration of the TTO used in this formulation is increased so that both treatments could have similar antibacterial action. Based on the results obtained in this research, TTO could be considered a therapeutic alternative in treating periodontal disease because it presents antimicrobial activity against periodontopathogenic bacteria and could be used for long periods. Nevertheless, it is necessary to complete clinical studies to evaluate its efficacy and possible adverse events.
publisher	Facultad de Odontología
publishDate	2025
url	https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/48695
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spelling	I10-R335-article-486952025-04-25T13:17:48Z Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study Evaluación de la actividad antimicrobiana en cuatro bacterias periodontopatógenas, de una formulación con aceite de árbol de té. Estudio in vitro Díaz Basurto, Diana D Macín Cabrera, Susana A Noguez Méndez, Norma A Rubio Martínez, Alejandro Juárez Sandoval, José tea tree oil periodontal disease periodontal pathogenic miroorganisms antimicrobial chlorexidine aceite de árbol de té enfermedad periodontal microorganismos patógenos periodontales antimicrobiano clorhexidina Natural products have been resurgent in recent years, intending to palliate oral ailments. For this reason, Tea Tree Oil (TTO) is proposed as the active ingredient of a gel for dental use, which can be used for periodontal disease. In this research, we compared TTO’s efficacy in contrast with chlorhexidine, against Porphyromonas gingivalis (P. gingivalis), Prevotella intermedia (P. intermedia), Fusobacterium nucleatum (F. nucleatum) and Parviromonas micra (P. micra). Dental plaque biofilm samples were collected from 30 subjects diagnosed with periodontal disease. Samples were subsequently cultivated, isolated, and verified. Then, the Kirby-Bauer method was used to evaluate the antimicrobial activity (n=360). The statistical test results indicated no statistically significant differences from chlorhexidine (CHX) for three bacteria: P. intermedia, F. nucleatum, and P. micra. In contrast, for P. gingivalis, there is a significant difference (p<0.001). However, this difference could be reduced if the concentration of the TTO used in this formulation is increased so that both treatments could have similar antibacterial action. Based on the results obtained in this research, TTO could be considered a therapeutic alternative in treating periodontal disease because it presents antimicrobial activity against periodontopathogenic bacteria and could be used for long periods. Nevertheless, it is necessary to complete clinical studies to evaluate its efficacy and possible adverse events. En los últimos años está resurgiendo el uso de productos naturales, con el objetivo de aliviar padecimientos de origen oral, por lo cual se propone el uso del Aceite de árbol de té (AAT), como principio activo de un gel de uso odontológico, que pueda ser utilizado para la enfermedad periodontal. En esta investigación se evaluó y comparó su eficacia contra Clorhexidina en cuatro bacterias periodontopatógenas: Porphyromonas gingivalis (P. gingivalis), Prevotella intermedia (P. intermedia), Fusobacterium nucleatum (F. nucleatum) y Parviromonas micra (P. micra), mediante un estudio in vitro, se realizó toma de muestras de biofilm de placa dental en pacientes con enfermedad periodontal (n=30), para poder cultivar, aislar y verificar cepas de bacterias periodontopatógenas, posteriormente se utilizó el método Kirby-Bauer para evaluar la actividad antimicrobiana (n=360). Los resultados de las pruebas estadísticas indicaron que no existen diferencias estadísticamente significativas en comparación con la Clorhexidina (CHX) para tres bacterias: P. intermedia, F. nucleatum y P. micra, mientras que para la bacteria P. gingivalis, si existe diferencia significativa (p<0,001), sin embargo, esta diferencia pudiera disminuirse, si se aumenta la concentración del AAT utilizada en esta formulación, por lo cual ambos tratamientos podrían tener acción antibacteriana similar. Con base en los resultados obtenidos en esta investigación pudiera considerarse como una alternativa terapéutica en el tratamiento de la enfermedad periodontal debido a que presenta actividad antimicrobiana contra las bacterias periodontopatógenas y podría emplearse por periodos largos de tiempo, aunque se requiere completar con estudios clínicos para evaluar su eficacia y posibles eventos adversos. Facultad de Odontología 2025-04-25 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/48695 Revista de la Facultad de Odontología; Vol. 35 Núm. 1 (2025): Revista de la Facultad de Odontología. Volumen 35 . Nro 1. 2025; 11-17 2545-7594 0325-1071 10.25014/ eng https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/48695/48851 10.25014/ https://creativecommons.org/licenses/by-nc-sa/4.0

Evaluation of the antimicrobial activity of a tea tree oil formulation on four periodontopathogenic bacteria. In vitro study

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