Effects of Low Power Laser Radiation on Rat Submandibular Gland Tumors: Histopathological and Histomorphometic

Salivary gland neoplasms affect the patient´s quality of life. Treatments for these tumors are quite restricted and present side effects. The cancer present, mutation wich producing an unrestrained cellular proliferation. Studies about the effects of Low Power Laser Therapy (LPLT) on salivary glands...

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Detalles Bibliográficos
Autores principales: Zanotti, Federico, Plavnik, Luis Mario
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Odontología 2023
Materias:
laser-rats-submandibular-tumors
Láser-Ratas-Submandibular-Tumores
Acceso en línea:https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/40380
Aporte de:
Revista de la Facultad de Odontología de Universidad Nacional de Córdoba
id I10-R335-article-40380
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-335
container_title_str Revista de la Facultad de Odontología
language Español
format Artículo revista
topic laser-rats-submandibular-tumors
Láser-Ratas-Submandibular-Tumores
spellingShingle laser-rats-submandibular-tumors
Láser-Ratas-Submandibular-Tumores
Zanotti, Federico
Plavnik, Luis Mario
Effects of Low Power Laser Radiation on Rat Submandibular Gland Tumors: Histopathological and Histomorphometic
topic_facet laser-rats-submandibular-tumors
Láser-Ratas-Submandibular-Tumores
author Zanotti, Federico
Plavnik, Luis Mario
author_facet Zanotti, Federico
Plavnik, Luis Mario
author_sort Zanotti, Federico
title Effects of Low Power Laser Radiation on Rat Submandibular Gland Tumors: Histopathological and Histomorphometic
title_short Effects of Low Power Laser Radiation on Rat Submandibular Gland Tumors: Histopathological and Histomorphometic
title_full Effects of Low Power Laser Radiation on Rat Submandibular Gland Tumors: Histopathological and Histomorphometic
title_fullStr Effects of Low Power Laser Radiation on Rat Submandibular Gland Tumors: Histopathological and Histomorphometic
title_full_unstemmed Effects of Low Power Laser Radiation on Rat Submandibular Gland Tumors: Histopathological and Histomorphometic
title_sort effects of low power laser radiation on rat submandibular gland tumors: histopathological and histomorphometic
description Salivary gland neoplasms affect the patient´s quality of life. Treatments for these tumors are quite restricted and present side effects. The cancer present, mutation wich producing an unrestrained cellular proliferation. Studies about the effects of Low Power Laser Therapy (LPLT) on salivary glands showed an apoptosis increase. So, LLT could be a recommended. therapyObjective: to study histopatologyc an histomorphometric the effects of higher RLBP doses on malignant tumors induced with DMBA on the submandibular glands of rats. Materials and Methods: Sixty (60) male Wistar rats with a corporal weight of ±120 g was divided into two groups of thirty (30) a Control Group (CG) and an Experimental Group (EG). Each animal was anesthetized to surgically expose the submandibular glands wich were injected with 50 uL of DMBA. The rats from the CG were kept alive for 150 days post injection and those from EG after 150 days post injection the glands were exposed and then were irradiated with a LPR for 6. 5 minutes, and necropsied 12 hours post radiation, and the submandibular glands were extracted. 30 were fixed, following routine procedure and tinted with hematoxylin-eosin stains. The tissues were histopathologically and histomorphometically studied Results: All the tumors, were myoepithelial carcinoma cells. In the experimental groups, an increase of tumorous apoptotic cells in relation to the control group were detected. These indications were corroborated with the statistics histomorphometric analysis Conclusion: the results of the current study allow us to claim that the effect of RLBP on submandibular glands is y increasing the apoptotic process.
publisher Facultad de Odontología
publishDate 2023
url https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/40380
work_keys_str_mv AT zanottifederico effectsoflowpowerlaserradiationonratsubmandibularglandtumorshistopathologicalandhistomorphometic
AT plavnikluismario effectsoflowpowerlaserradiationonratsubmandibularglandtumorshistopathologicalandhistomorphometic
AT zanottifederico efectosdelasradiacioneslaserdebajapotenciasobretumoresdeglandulassubmandibularesderataestudioshistopatologicoehistomorfometrico
AT plavnikluismario efectosdelasradiacioneslaserdebajapotenciasobretumoresdeglandulassubmandibularesderataestudioshistopatologicoehistomorfometrico
first_indexed 2024-09-03T21:16:30Z
last_indexed 2024-09-03T21:16:30Z
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spelling I10-R335-article-403802023-03-01T19:01:45Z Effects of Low Power Laser Radiation on Rat Submandibular Gland Tumors: Histopathological and Histomorphometic Efectos de las Radiaciones Laser de Baja Potencia sobre tumores de glándulas submandibulares de rata: Estudios histopatológico e histomorfométrico Zanotti, Federico Plavnik, Luis Mario laser-rats-submandibular-tumors Láser-Ratas-Submandibular-Tumores Salivary gland neoplasms affect the patient´s quality of life. Treatments for these tumors are quite restricted and present side effects. The cancer present, mutation wich producing an unrestrained cellular proliferation. Studies about the effects of Low Power Laser Therapy (LPLT) on salivary glands showed an apoptosis increase. So, LLT could be a recommended. therapyObjective: to study histopatologyc an histomorphometric the effects of higher RLBP doses on malignant tumors induced with DMBA on the submandibular glands of rats. Materials and Methods: Sixty (60) male Wistar rats with a corporal weight of ±120 g was divided into two groups of thirty (30) a Control Group (CG) and an Experimental Group (EG). Each animal was anesthetized to surgically expose the submandibular glands wich were injected with 50 uL of DMBA. The rats from the CG were kept alive for 150 days post injection and those from EG after 150 days post injection the glands were exposed and then were irradiated with a LPR for 6. 5 minutes, and necropsied 12 hours post radiation, and the submandibular glands were extracted. 30 were fixed, following routine procedure and tinted with hematoxylin-eosin stains. The tissues were histopathologically and histomorphometically studied Results: All the tumors, were myoepithelial carcinoma cells. In the experimental groups, an increase of tumorous apoptotic cells in relation to the control group were detected. These indications were corroborated with the statistics histomorphometric analysis Conclusion: the results of the current study allow us to claim that the effect of RLBP on submandibular glands is y increasing the apoptotic process. Las neoplasias de glándulas salivales afectan la calidad de vida del paciente. Los tratamientos son restringidos y poseen numerosos efectos secundarios. En el cáncer, aparecen mutaciones que producen una proliferación celular desenfrenada Estudios del efecto de altas densidades de Radiaciones Laser de Baja Potencia (RLBP) sobre glándulas salivales mostraron aumento de la apoptosis, siendo estas radiaciones un tratamiento alternativo. Objetivo: Estudiar histopatológica e histomorfométricamente los efectos que las RLBP, en densidades de energía altas, producen sobre tumores malignos inducidos con DMBA, en glándulas submandibulares de rata. Materiales y métodos: Se utilizaron sesenta ratas macho Wistar de ±120 g de peso corporal distribuidos en dos grupos de 30 animales: Grupo control (GC) y Grupo experimental (GE). Ambos grupos fueron anestesiados y se expusieron las glándulas submandibulares, inyectándoseles 50 µl de DMBA. Las del GC se mantuvieron vivas durante 150 días post inyección; y las del GE también 150 días post inyección y luego, se les aplicó laser de He-Ne durante 6,5 minutos, sacrificándolas a las 12 horas post irradiación. Finalizado el tiempo experimental se extirparon las glándulas submandibulares. Las glándulas derechas fueron fijadas en formol al 10% tamponado y procesadas según la técnica de rutina, obteniéndose cortes seriados teñidos con Hematoxilina-Eosina para estudios histopatológicos e histomorfométricos. Resultados: Todos fueron carcinomas a células mioepiteliales. Histopatológica e histomorfometricamente se observó que en GE aumentaron las células tumorales apoptóticas en relación al GC. Conclusión: Estos hallazgos indican que las RLBP sobre tumores de glándulas submandibulares de ratas, modifican el ciclo celular exacerbando el proceso apoptótico. Facultad de Odontología 2023-03-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/40380 Revista de la Facultad de Odontología; Vol. 33 Núm. 1 (2023): Rev Fac Odont 33(1)-2023-Marzo; 38-46 2545-7594 0325-1071 spa https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/40380/40628 https://creativecommons.org/licenses/by-nc-sa/4.0

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