Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats
The objective of the present work was to evaluate the effect of carboplatin (Cp) on submandibular gland homogenate of Wistar rats through the determination of malondialdehyde levels, as the main end product of lipoperoxidation, in an experimental model. Sixteen three-month-old male Wistar rats were...
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Facultad de Odontología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/34295 |
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I10-R335-article-34295 |
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| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-335 |
| container_title_str |
Revista de la Facultad de Odontología |
| language |
Español |
| format |
Artículo revista |
| topic |
Witar rats carboplatin malondialdehyde submandibular gland ratas Wistar carboplatino malondialdehido glándula submandibular |
| spellingShingle |
Witar rats carboplatin malondialdehyde submandibular gland ratas Wistar carboplatino malondialdehido glándula submandibular Bachmeier, Evelin Migueles Goitea , María Elena Linares, Jorge Alberto Ferreyra Mariani, Emma G Wietz, Fernando Dubersarsky, Claudio Gastón Brunotto, Mabel Mazzeo, Marcelo Adrián Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats |
| topic_facet |
Witar rats carboplatin malondialdehyde submandibular gland ratas Wistar carboplatino malondialdehido glándula submandibular |
| author |
Bachmeier, Evelin Migueles Goitea , María Elena Linares, Jorge Alberto Ferreyra Mariani, Emma G Wietz, Fernando Dubersarsky, Claudio Gastón Brunotto, Mabel Mazzeo, Marcelo Adrián |
| author_facet |
Bachmeier, Evelin Migueles Goitea , María Elena Linares, Jorge Alberto Ferreyra Mariani, Emma G Wietz, Fernando Dubersarsky, Claudio Gastón Brunotto, Mabel Mazzeo, Marcelo Adrián |
| author_sort |
Bachmeier, Evelin |
| title |
Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats |
| title_short |
Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats |
| title_full |
Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats |
| title_fullStr |
Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats |
| title_full_unstemmed |
Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats |
| title_sort |
effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats |
| description |
The objective of the present work was to evaluate the effect of carboplatin (Cp) on submandibular gland homogenate of Wistar rats through the determination of malondialdehyde levels, as the main end product of lipoperoxidation, in an experimental model. Sixteen three-month-old male Wistar rats were used, housed in individual cages, with controlled temperature and lighting and free diet. A completely randomized design was used and two experimental groups were established: 1) Control (C), administering an intraperitoneal dose of saline solution for one day, n: 8, 2) Animals treated with carboplatin (Cp) applying a dose i.p. of 100 mg / Kg of body weight for one day, n: 8. The animals were fasted for 24 hours and subsequently anesthetized. Then both submandibular glands were removed. Malondialdehyde levels were analyzed in submandibular gland homogenate in both experimental groups. Variations between the groups analyzed were evaluated using the Student's t test for paired samples, setting a p-value <0.05 for statistical significance. Project approved by CICUAL. Faculty of Medical Sciences (UNC). The group of Rats C o showed a concentration of 7.32 ± 0.48 µmol / mg of gland. The Cp group had a concentration of 12.57 ± 0.71 µmol / mg of gland, expressing a significant decrease compared to the control group p <0.0006. Cp at the dose tested would cause a decrease in lipoperoxidation in the submandibular gland of rats. Possibly the glandular antioxidant battery would neutralize the oxidative stress of acinar cells. These results suggest a future evaluation of superoxide dismutase (SOD) activity and uric acid (UA) levels |
| publisher |
Facultad de Odontología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/34295 |
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2024-09-03T21:16:22Z |
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I10-R335-article-342952025-12-02T15:49:09Z Effect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats Efecto de carboplatino sobre malondialdehído como marcador de lipoderoxidación en glándula submandibular de ratas Bachmeier, Evelin Migueles Goitea , María Elena Linares, Jorge Alberto Ferreyra Mariani, Emma G Wietz, Fernando Dubersarsky, Claudio Gastón Brunotto, Mabel Mazzeo, Marcelo Adrián Witar rats carboplatin malondialdehyde submandibular gland ratas Wistar carboplatino malondialdehido glándula submandibular The objective of the present work was to evaluate the effect of carboplatin (Cp) on submandibular gland homogenate of Wistar rats through the determination of malondialdehyde levels, as the main end product of lipoperoxidation, in an experimental model. Sixteen three-month-old male Wistar rats were used, housed in individual cages, with controlled temperature and lighting and free diet. A completely randomized design was used and two experimental groups were established: 1) Control (C), administering an intraperitoneal dose of saline solution for one day, n: 8, 2) Animals treated with carboplatin (Cp) applying a dose i.p. of 100 mg / Kg of body weight for one day, n: 8. The animals were fasted for 24 hours and subsequently anesthetized. Then both submandibular glands were removed. Malondialdehyde levels were analyzed in submandibular gland homogenate in both experimental groups. Variations between the groups analyzed were evaluated using the Student's t test for paired samples, setting a p-value <0.05 for statistical significance. Project approved by CICUAL. Faculty of Medical Sciences (UNC). The group of Rats C o showed a concentration of 7.32 ± 0.48 µmol / mg of gland. The Cp group had a concentration of 12.57 ± 0.71 µmol / mg of gland, expressing a significant decrease compared to the control group p <0.0006. Cp at the dose tested would cause a decrease in lipoperoxidation in the submandibular gland of rats. Possibly the glandular antioxidant battery would neutralize the oxidative stress of acinar cells. These results suggest a future evaluation of superoxide dismutase (SOD) activity and uric acid (UA) levels El objetivo del presente trabajo fue evaluar el efecto de carboplatino (Cp) en homogenato de glándula submandibular de ratas Wistar a través de la determinación de los niveles de malondialdehido (MDA), como principal producto final de la lipoperoxidación, en un modelo experimental. Se utilizaron 16 ratas Wistar macho de tres meses de edad, alojadas en jaulas individuales, con temperatura e iluminación controlada y dieta libre. Se utilizó un diseño completamente aleatorizado y se establecieron dos grupos experimentales: 1) Control (C), administrándose una dosis intraperitoneal de solución salina durante un día, n: 8, 2) Animales tratados con carboplatino (Cp) aplicándose una dosis i.p. de 100 mg/Kg de peso corporal durante un día, n: 8. Los animales fueron ayunados por 24 horas y posteriormente anestesiados. Seguidamente se extirparon ambas glándulas submandibulares. Se analizaron los niveles de malondialdehido en homogenato de glándula submandibular en ambos grupos experimentales. Las variaciones entre los grupos analizados se evaluaron mediante prueba T de Student para muestras apareadas, fijando un p-valor <0,05 para significación estadística. Proyecto aprobado por CICUAL. Facultad de Ciencias Médicas (UNC). El grupo de Ratas C mostró una concentración de 7.32 ± 0.48 µmol/mg de glándula. El grupo Cp tuvo una concentración de 12.57 ± 0,71 µmol/mg de glándula, expresando una disminución significativa respecto del grupo control p<0.0006. Cp en la dosis ensayada provocaría una disminución de la lipoperoxidación en glándula submandibular de ratas. Posiblemente la batería antioxidante glandular neutralizaría el estrés oxidativo de las células acinares. Estos resultados sugieren evaluar a futuro la actividad de superóxido dismutasa (SOD) y niveles de ácido úrico (AU). Facultad de Odontología 2021-08-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/34295 Revista de la Facultad de Odontología; Vol. 31 Núm. 2 (2021): Rev Fac Odont 31(2)-2021-Agosto; 9-13 2545-7594 0325-1071 spa https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/34295/34710 10.25014/ Derechos de autor 2021 Evelin Bachmeier, María Elena Migueles Goitea , Jorge Alberto Linares, Emma G Ferreyra Mariani, Fernando Wietz, Claudio Gastón Dubersarsky, Mabel Brunotto, Marcelo Adrián Mazzeo https://creativecommons.org/licenses/by/4.0 |