Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats
Objective: the aim of this study was to analyze salivary and plasma fatty acid (FA) levels after their immediate intake in rats.Methods: 6 adult male Wistar rats were fed on commercial diet until the 12th week of life, in which it was replaced by a laboratory diet containing corn oil (6%) as a lipid...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Facultad de Odontología
2020
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/31182 |
| Aporte de: |
| id |
I10-R335-article-31182 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-335 |
| container_title_str |
Revista de la Facultad de Odontología |
| language |
Español |
| format |
Artículo revista |
| topic |
fatty acids, saliva, plasma ácidos grasos saliva plasma |
| spellingShingle |
fatty acids, saliva, plasma ácidos grasos saliva plasma Combina, César Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats |
| topic_facet |
fatty acids, saliva, plasma ácidos grasos saliva plasma |
| author |
Combina, César |
| author_facet |
Combina, César |
| author_sort |
Combina, César |
| title |
Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats |
| title_short |
Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats |
| title_full |
Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats |
| title_fullStr |
Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats |
| title_full_unstemmed |
Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats |
| title_sort |
salivary and plasmatic incorporation of fatty acids after their immediate intake in rats |
| description |
Objective: the aim of this study was to analyze salivary and plasma fatty acid (FA) levels after their immediate intake in rats.Methods: 6 adult male Wistar rats were fed on commercial diet until the 12th week of life, in which it was replaced by a laboratory diet containing corn oil (6%) as a lipid source (linoleic 18: 2 n-6 FA). At 12 and 24 hours after the diet change, rats were anesthetized and salivary secretion was induced by an intraperitoneal injection of isoproterenol / pilocarpine (5 mg/kg each) before their sacrifice. Total saliva (S) was collected for 20 min by using intraoral cotton rolls. Blood was obtained (cardiac puncture) to separate plasma (P) (centrifugation). S and P lipids were extracted for FA methylation and analysis by gas chromatography - mass spectrometry. Spearman correlation and linear regression coefficients (p≤0.05) were applied. Results: 18:2 n-6 FA was detected in S and P at 12 and 24 hours, being higher at 24 hours and in P. A higher number of FA was also found at 12 and 24 hours both in P than in S. 24/12 hour concentration ratios in S were 16:0 (1.20); 16:1 (1.65); 18:0 (0.33); 18:1 n-9 (1.55); 18:2 n-6 (1.70); 20:4 n-6 (1.20) and 22:6 n-3 (1.19) and in P: 16:0 (0.49); 16:1 (4.84); 18:0 (3.43); 18:1 n-9 (3.14); 18:2 n-6 (1.75); 20:4 n-6 (0.52) and 22:6 n-3 (0.73). A significant positive correlation between S and P 16:1 n-9 FA and a high regression between 16:0;16:1 n-9 and 18:0 salivary/plasma FA was found. Conclusion: this preliminary study showed that the 18:2 n-6 FA intake is rapidly reflected in rat S and P, with a time increase, and that there is a correlation between some FA of both fluids. |
| publisher |
Facultad de Odontología |
| publishDate |
2020 |
| url |
https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/31182 |
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AT combinacesar salivaryandplasmaticincorporationoffattyacidsaftertheirimmediateintakeinrats AT combinacesar incorporacionsalivalyplasmaticadeacidosgrasosluegodesuingestainmediataenratas |
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2024-09-03T21:16:19Z |
| last_indexed |
2024-09-03T21:16:19Z |
| _version_ |
1809211121821810688 |
| spelling |
I10-R335-article-311822020-12-08T11:49:51Z Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats Incorporación salival y plasmática de ácidos grasos luego de su ingesta inmediata en ratas Combina, César fatty acids, saliva, plasma ácidos grasos saliva plasma Objective: the aim of this study was to analyze salivary and plasma fatty acid (FA) levels after their immediate intake in rats.Methods: 6 adult male Wistar rats were fed on commercial diet until the 12th week of life, in which it was replaced by a laboratory diet containing corn oil (6%) as a lipid source (linoleic 18: 2 n-6 FA). At 12 and 24 hours after the diet change, rats were anesthetized and salivary secretion was induced by an intraperitoneal injection of isoproterenol / pilocarpine (5 mg/kg each) before their sacrifice. Total saliva (S) was collected for 20 min by using intraoral cotton rolls. Blood was obtained (cardiac puncture) to separate plasma (P) (centrifugation). S and P lipids were extracted for FA methylation and analysis by gas chromatography - mass spectrometry. Spearman correlation and linear regression coefficients (p≤0.05) were applied. Results: 18:2 n-6 FA was detected in S and P at 12 and 24 hours, being higher at 24 hours and in P. A higher number of FA was also found at 12 and 24 hours both in P than in S. 24/12 hour concentration ratios in S were 16:0 (1.20); 16:1 (1.65); 18:0 (0.33); 18:1 n-9 (1.55); 18:2 n-6 (1.70); 20:4 n-6 (1.20) and 22:6 n-3 (1.19) and in P: 16:0 (0.49); 16:1 (4.84); 18:0 (3.43); 18:1 n-9 (3.14); 18:2 n-6 (1.75); 20:4 n-6 (0.52) and 22:6 n-3 (0.73). A significant positive correlation between S and P 16:1 n-9 FA and a high regression between 16:0;16:1 n-9 and 18:0 salivary/plasma FA was found. Conclusion: this preliminary study showed that the 18:2 n-6 FA intake is rapidly reflected in rat S and P, with a time increase, and that there is a correlation between some FA of both fluids. Objetivo: analizar los niveles de ácidos grasos (AG) salivales y plasmáticos luego de su ingesta inmediata en ratas. Métodos: 6 ratas Wistar machos adultas recibieron dieta comercial hasta la 12ª semana de vida, en que se la reemplazó por dieta de laboratorio con aceite de maíz (6%) como fuente lipídica (principal AG linoleico 18:2 n-6). A 12 y 24 hs del cambio de dieta las ratas fueron anestesiadas y, antes de su sacrificio, se indujo la secreción salival mediante inyección intraperitoneal de isoproterenol/pilocarpina (5 mg/kg de c/u). La saliva (S) total fue recolectada durante 20 min mediante rollos de algodón intrabucales. Se obtuvo sangre (punción cardíaca) para separar plasma (P) (centrifugación). Se extrajeron lípidos de S y P para metilación de AG y análisis por cromatografía de gas–espectometría de masa. Se aplicaron coeficientes de correlación de Spearman y de regresión lineal (p≤0,05). Resultados: el AG 18:2 n-6 fue hallado en S y P a 12 y 24 hs, siendo mayor a 24 hs y en P. También se encontró un mayor número de AG en P que en S, a 12 y 24 hs. Los cocientes de concentraciones 24 hs/12hs en S fueron 16:0 (1,20); 16:1 (1,65); 18:0 (0,33); 18:1 n-9 (1,55); 18:2 n-6 (1,70); 20:4 n-6 (1,20) y 22:6 n-3 (1,19) y en P 16:0 (0,49); 16:1 (4,84); 18:0 (3,43); 18:1 n-9 (3,14); 18:2 n-6 (1,75); 20:4 n-6 (0,52) y 22:6 n-3 (0,73). Se observó una correlación positiva significativa entre AG 16:1 n-9 de S y P y una alta regresión entre AG 16:0; 16:1 n-9 y 18:0 salivales/plasmáticos. Conclusión: este estudio preliminar mostró que la ingesta del AG 18:2 n-6 se refleja rápidamente en S y P de ratas, con aumento en el tiempo, y que existe una correlación entre algunos AG de esos fluidos. Facultad de Odontología 2020-12-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/31182 Revista de la Facultad de Odontología; Vol. 30 Núm. 3 (2020): Rev Fac Odont vol 30 (3) 2020; 29-35 2545-7594 0325-1071 spa https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/31182/31843 https://creativecommons.org/licenses/by-nc-sa/4.0 |