Structural changes induced by he-ne laser on the chick embryo ovary
The morpho-histochemical alterations that occur in the ehicken ovary at 7 days of incubation after irradiation with He-Ne laser of a petency of 5 mw and at a wavclength of 632.8 nm were studied. The embryos were irradiatcd for 5 minutes through a window opcned in the eggshc.11 and aseptically mainta...
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| Autores principales: | , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
1992
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/44365 |
| Aporte de: |
| Sumario: | The morpho-histochemical alterations that occur in the ehicken ovary at 7 days of incubation after irradiation with He-Ne laser of a petency of 5 mw and at a wavclength of 632.8 nm were studied. The embryos were irradiatcd for 5 minutes through a window opcned in the eggshc.11 and aseptically maintained in incuhator for 24 liours. The gonads were dissected uní and processed for the following techniques: H / E, PAS, Alcian blue and Toluidine bine. Controls: The ovaries were fornied by a germinative or superficial epithelium with germ and epithelial celis, and by primary sex cords cornpressed between them, although separated by a reducedstroma. The cords contained germ celis The surface coat of the germinative epitheliurn presented a thin iayer of PAS positive, alcianophilic at pH 25 and ortochromatic material. Basemen niembranes and intercorci extracellular substance were also PAS positive. Prohiems: Disorganization of the tissue structure was well manifest in irradiated gonads, accoinpanicd by negativization of the histochernical reactions. A Iymphocytary infiltration was als found, No structural alteratioris were observed in germ or epíhelial celis. It is concluded that laser radiations would act producing deerease of the muco- snbstances associated t) the plasma membrane and basement membrane. They would also provoke the appea. rance of an inflammatory mononucleai infiltration. |
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