Structural changes induced by he-ne laser on the chick embryo ovary

The morpho-histochemical alterations that occur in the ehicken ovary at 7 days of incubation after irradiation with He-Ne laser of a petency of 5 mw and at a wavclength of 632.8 nm were studied. The embryos were irradiatcd for 5 minutes through a window opcned in the eggshc.11 and aseptically mainta...

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Autores principales: ávila, Rodolfo, Samar, María Elena, Juri, Hugo, Fabro, Sofía, Cneturión, Claudio, Sanchez Mazzafferro, Rafael
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 1992
Materias:
chick embyo
ovaries
he-ne laser irradiation
embrión de pollo
ovarios
irradiación de láser HE-NE
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/44365
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-44365
record_format ojs
spelling I10-R327-article-443652024-11-27T20:43:00Z Structural changes induced by he-ne laser on the chick embryo ovary ávila, Rodolfo Samar, María Elena Juri, Hugo Fabro, Sofía Cneturión, Claudio Sanchez Mazzafferro, Rafael chick embyo ovaries he-ne laser irradiation embrión de pollo ovarios irradiación de láser HE-NE The morpho-histochemical alterations that occur in the ehicken ovary at 7 days of incubation after irradiation with He-Ne laser of a petency of 5 mw and at a wavclength of 632.8 nm were studied. The embryos were irradiatcd for 5 minutes through a window opcned in the eggshc.11 and aseptically maintained in incuhator for 24 liours. The gonads were dissected uní and processed for the following techniques: H / E, PAS, Alcian blue and Toluidine bine. Controls: The ovaries were fornied by a germinative or superficial epithelium with germ and epithelial celis, and by primary sex cords cornpressed between them, although separated by a reducedstroma. The cords contained germ celis The surface coat of the germinative epitheliurn presented a thin iayer of PAS positive, alcianophilic at pH 25 and ortochromatic material. Basemen niembranes and intercorci extracellular substance were also PAS positive. Prohiems: Disorganization of the tissue structure was well manifest in irradiated gonads, accoinpanicd by negativization of the histochernical reactions. A Iymphocytary infiltration was als found, No structural alteratioris were observed in germ or epíhelial celis. It is concluded that laser radiations would act producing deerease of the muco- snbstances associated t) the plasma membrane and basement membrane. They would also provoke the appea. rance of an inflammatory mononucleai infiltration. Se estudiaron las modificaciones morfohistoquímicas que ocurren en el evario de pollo de 7 días de incubación por iiadiación con laser He-Nc de 5 mw de potencia y 632,8 nm de longitud de onda. Los embriones fueron irradiados durante 5 minutos a través detina ventana abierta en la cáscara del huevo y mantenidos asépticamente duiante 24 horas en incubadora. Se divecaron las gónadas y se procesaron para las técnicas de H,/E, PAS, Alejan Bkie y Azul de tohiidina. Controles: Los ovarios estaban formados por un epitelio germinativo o superficial, con células germnales y epiteliales, y por cordones sexuales unmanos apretados tinos contra otros, aunque separados por im estroma niuv reóucido. Los cordones contenían células germinales. La cubierta superficial del epilelio germinativo presentaba una delgada capa de material PAS positivo alcianófilo a pH 2,5 y ortocromático. Las láminas basales y la sustancia extracelular intercordonal también erin PAS positivas. Problemas: En las gónadas irradiodas era bien manifiesta la desorganzae6n de la estructura tisular, acompañaca de una negativización de las reacciones histoquímicas También se observó un infiltrado linfocitanio. En las células germinales y epiteliales no se observaron alteraciones estructurales. Se concluye que las radiaciones laser actuarían produciendo disminución d ias mucosustancias asociadas a la membrana plasmática y membranas basales.Provocarían además, la aparición de un infiltrado inflamatorio mononuclear. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 1992-05-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/44365 10.31053/1853.0605.v50.n1.44365 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 50 No. 1 (1992); 7-11 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 50 Núm. 1 (1992); 7-11 Revista da Faculdade de Ciências Médicas de Córdoba; v. 50 n. 1 (1992); 7-11 1853-0605 0014-6722 10.31053/1853.0605.v50.n1 spa https://revistas.unc.edu.ar/index.php/med/article/view/44365/45065 Derechos de autor 2024 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic chick embyo
ovaries
he-ne laser irradiation
embrión de pollo
ovarios
irradiación de láser HE-NE
spellingShingle chick embyo
ovaries
he-ne laser irradiation
embrión de pollo
ovarios
irradiación de láser HE-NE
ávila, Rodolfo
Samar, María Elena
Juri, Hugo
Fabro, Sofía
Cneturión, Claudio
Sanchez Mazzafferro, Rafael
Structural changes induced by he-ne laser on the chick embryo ovary
topic_facet chick embyo
ovaries
he-ne laser irradiation
embrión de pollo
ovarios
irradiación de láser HE-NE
author ávila, Rodolfo
Samar, María Elena
Juri, Hugo
Fabro, Sofía
Cneturión, Claudio
Sanchez Mazzafferro, Rafael
author_facet ávila, Rodolfo
Samar, María Elena
Juri, Hugo
Fabro, Sofía
Cneturión, Claudio
Sanchez Mazzafferro, Rafael
author_sort ávila, Rodolfo
title Structural changes induced by he-ne laser on the chick embryo ovary
title_short Structural changes induced by he-ne laser on the chick embryo ovary
title_full Structural changes induced by he-ne laser on the chick embryo ovary
title_fullStr Structural changes induced by he-ne laser on the chick embryo ovary
title_full_unstemmed Structural changes induced by he-ne laser on the chick embryo ovary
title_sort structural changes induced by he-ne laser on the chick embryo ovary
description The morpho-histochemical alterations that occur in the ehicken ovary at 7 days of incubation after irradiation with He-Ne laser of a petency of 5 mw and at a wavclength of 632.8 nm were studied. The embryos were irradiatcd for 5 minutes through a window opcned in the eggshc.11 and aseptically maintained in incuhator for 24 liours. The gonads were dissected uní and processed for the following techniques: H / E, PAS, Alcian blue and Toluidine bine. Controls: The ovaries were fornied by a germinative or superficial epithelium with germ and epithelial celis, and by primary sex cords cornpressed between them, although separated by a reducedstroma. The cords contained germ celis The surface coat of the germinative epitheliurn presented a thin iayer of PAS positive, alcianophilic at pH 25 and ortochromatic material. Basemen niembranes and intercorci extracellular substance were also PAS positive. Prohiems: Disorganization of the tissue structure was well manifest in irradiated gonads, accoinpanicd by negativization of the histochernical reactions. A Iymphocytary infiltration was als found, No structural alteratioris were observed in germ or epíhelial celis. It is concluded that laser radiations would act producing deerease of the muco- snbstances associated t) the plasma membrane and basement membrane. They would also provoke the appea. rance of an inflammatory mononucleai infiltration.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 1992
url https://revistas.unc.edu.ar/index.php/med/article/view/44365
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AT cneturionclaudio structuralchangesinducedbyhenelaseronthechickembryoovary
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first_indexed 2025-02-05T22:05:31Z
last_indexed 2025-02-05T22:05:31Z
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