Mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in Chagas disease.

The entry of Trypanosoma cruzi (T.cruzi) into cells causes inflammatory reactions that stimulate the production of cytokines and free radicals responsible for oxidative damage; which could lead to heart failure. We studied the activity of complexes I and III (CI, CIII) of the respiratory chain in mi...

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Autores principales: Báez , AL, Lo Presti , MS, Bazán, PC, Velázquez López , DA, Rivarola, HW, Paglini, PA
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023
Materias:
Chagas disease
mitochondrial enzyme activity
lymphomonocyte fraction
Enfermedad de Chagas
actividad enzimática mitocondrial
fracción linfomonocitaria
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/42653
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-42653
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institution Universidad Nacional de Córdoba
institution_str I-10
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container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic Chagas disease
mitochondrial enzyme activity
lymphomonocyte fraction
Enfermedad de Chagas
actividad enzimática mitocondrial
fracción linfomonocitaria
spellingShingle Chagas disease
mitochondrial enzyme activity
lymphomonocyte fraction
Enfermedad de Chagas
actividad enzimática mitocondrial
fracción linfomonocitaria
Báez , AL
Lo Presti , MS
Bazán, PC
Velázquez López , DA
Rivarola, HW
Paglini, PA
Mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in Chagas disease.
topic_facet Chagas disease
mitochondrial enzyme activity
lymphomonocyte fraction
Enfermedad de Chagas
actividad enzimática mitocondrial
fracción linfomonocitaria
author Báez , AL
Lo Presti , MS
Bazán, PC
Velázquez López , DA
Rivarola, HW
Paglini, PA
author_facet Báez , AL
Lo Presti , MS
Bazán, PC
Velázquez López , DA
Rivarola, HW
Paglini, PA
author_sort Báez , AL
title Mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in Chagas disease.
title_short Mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in Chagas disease.
title_full Mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in Chagas disease.
title_fullStr Mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in Chagas disease.
title_full_unstemmed Mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in Chagas disease.
title_sort mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in chagas disease.
description The entry of Trypanosoma cruzi (T.cruzi) into cells causes inflammatory reactions that stimulate the production of cytokines and free radicals responsible for oxidative damage; which could lead to heart failure. We studied the activity of complexes I and III (CI, CIII) of the respiratory chain in mitochondria of the lymphomonocyte fraction, of experimental models infected with different strains of T. cruzi, and in patients with positive serology (long standing) for the disease of Chagas, with the objective of determining the participation of mitochondria as probable markers of early evolution and clinical variability of cardiomyopathy produced by T. cruzi. Albino Swiss mice were infected with the Tulahuen strain and the SGOZ12 and Lucky isolates (n=30 per group); controls (n=30). Patients: n=55 (43 with positive serology, 12 controls) grouped into: Chagas 1 (<40 years), Chagas 2 (>45) and controls 1 and 2 (healthy of both ages). The enzymatic activity was determined by spectrophotometry, the data was analyzed by ANOVA, Fisher Test. Significance level p<0.05. In the experimental models, CI and III were modified in the infected groups (p<0.05) at all stages, depending on the strain that infected the host. In the group of patients, a significant decrease in activity was observed among the controls (p<0.05), which would show that it decreases with age. While in chagasic patients a decrease in enzyme activity was observed in the older group (Chagas 2), although this difference did not become significant when compared with the Chagas 1 group (<40 years), if instead when compared with the Control group 1 (<40 years). Inflammatory processes are responsible for causing changes in the mitochondria and can be detected by a blood sample. These changes would be important since, by appearing before the clinical manifestation, they could prevent the evolution of the disease, and the lymphomonocyte fraction could be a simple marker that helps to stop the evolution towards Chagasic cardiomyopathy, especially in the chronic stage without symptomatology.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2023
url https://revistas.unc.edu.ar/index.php/med/article/view/42653
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first_indexed 2024-09-03T21:04:42Z
last_indexed 2024-09-03T21:04:42Z
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spelling I10-R327-article-426532023-10-19T21:20:20Z Mitochondrial enzymatic activity of the lymphomonocyte fraction as an early marker of pathology in Chagas disease. La actividad enzimática mitocondrial de la fracción linfomonocitaria como marcador temprano de patología en la enfermedad de Chagas Báez , AL Lo Presti , MS Bazán, PC Velázquez López , DA Rivarola, HW Paglini, PA Chagas disease mitochondrial enzyme activity lymphomonocyte fraction Enfermedad de Chagas actividad enzimática mitocondrial fracción linfomonocitaria The entry of Trypanosoma cruzi (T.cruzi) into cells causes inflammatory reactions that stimulate the production of cytokines and free radicals responsible for oxidative damage; which could lead to heart failure. We studied the activity of complexes I and III (CI, CIII) of the respiratory chain in mitochondria of the lymphomonocyte fraction, of experimental models infected with different strains of T. cruzi, and in patients with positive serology (long standing) for the disease of Chagas, with the objective of determining the participation of mitochondria as probable markers of early evolution and clinical variability of cardiomyopathy produced by T. cruzi. Albino Swiss mice were infected with the Tulahuen strain and the SGOZ12 and Lucky isolates (n=30 per group); controls (n=30). Patients: n=55 (43 with positive serology, 12 controls) grouped into: Chagas 1 (<40 years), Chagas 2 (>45) and controls 1 and 2 (healthy of both ages). The enzymatic activity was determined by spectrophotometry, the data was analyzed by ANOVA, Fisher Test. Significance level p<0.05. In the experimental models, CI and III were modified in the infected groups (p<0.05) at all stages, depending on the strain that infected the host. In the group of patients, a significant decrease in activity was observed among the controls (p<0.05), which would show that it decreases with age. While in chagasic patients a decrease in enzyme activity was observed in the older group (Chagas 2), although this difference did not become significant when compared with the Chagas 1 group (<40 years), if instead when compared with the Control group 1 (<40 years). Inflammatory processes are responsible for causing changes in the mitochondria and can be detected by a blood sample. These changes would be important since, by appearing before the clinical manifestation, they could prevent the evolution of the disease, and the lymphomonocyte fraction could be a simple marker that helps to stop the evolution towards Chagasic cardiomyopathy, especially in the chronic stage without symptomatology. El ingreso del Trypanosoma cruzi (T.cruzi) a las células produce reacciones inflamatorias que estimulan la producción de citoquinas y radicales libres responsables del daño oxidativo; lo que podría llevar a la insuficiencia cardíaca. Estudiamos la actividad de los complejos I y III (CI, CIII) de la cadena respiratoria en mitocondrias de la fracción linfomonocitaria, de modelos experimentales infectados con diferentes cepas de T. cruzi, y en pacientes con serología positiva (de larga data) para enfermedad de Chagas, con el objetivo de determinar la participación de las mitocondrias como marcadores probables de evolución temprana y de variabilidad clínica de la miocardiopatía producida por T. cruzi. Ratones Albino Swiss fueron infectados con cepa Tulahuen y aislamientos SGOZ12 y Lucky (n=30 por grupo); control (n=30). Pacientes: n=55 (43 con serología positiva, 12 controles) agrupados en: Chagas 1 (<40 años), Chagas 2 (>45) y controles 1 y 2 (sanos de ambas edades). La actividad enzimática se determinó por espectrofotometría, los datos se analizaron por ANOVA, Fisher Test. Nivel de significación p<0.05. En los modelos experimentales, CI y III se modificaron en los grupos infectados (p<0.05) en todas las etapas, según la cepa que infectó al huésped. En el grupo de pacientes se observó una disminución significativa de la actividad entre los controles (p<0,05), lo que demostraría que con la edad ésta disminuye. Mientras que en los pacientes chagásicos se observó disminución de la actividad enzimática en el grupo de mayor edad (Chagas 2), aunque no llegó a ser significativa esta diferencia comparándola con el grupo Chagas 1 (<40 años) , si en cambio comparándola con el grupo Control 1 (<40 años). Los procesos inflamatorios son responsables de provocar modificaciones en las mitocondrias pudiendo ser detectadas mediante una extracción de sangre. Estos cambios serían importantes ya que, al aparecer antes de la manifestación clínica podrían prevenir la evolución de la enfermedad, pudiendo ser, la fracción linfomonocitaria, un marcador sencillo que ayude a frenar la evolución hacia la miocardiopatía chagásica, especialmente en la etapa crónica sin sintomatología. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-10-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/42653 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 (2023): Suplemento JIC XXIV Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 (2023): Suplemento JIC XXIV Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 (2023): Suplemento JIC XXIV 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/42653/42855 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0

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