Photodynamic effect of Soranjidiol on intracellular amastigotes of Leishmania braziliensis
The study of new treatments on the amastigote form (A) of Leishmania is important, since these multiply within macrophages (M), spreading the infection within humans. Soranjidiol (Sor) is a compound (genus Heterophyllaea) that possesses photosensitizing properties. The effect of So...
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| Autores principales: | , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2023
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/42646 |
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I10-R327-article-42646 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
leishmania treatment Soranjidiol photodynamic effect leishmania tratamiento Soranjidiol efecto fotodinamico |
| spellingShingle |
leishmania treatment Soranjidiol photodynamic effect leishmania tratamiento Soranjidiol efecto fotodinamico Dimmer, JA Barrionuevo, CN Rivarola , HW Núñez Montoya, SC Photodynamic effect of Soranjidiol on intracellular amastigotes of Leishmania braziliensis |
| topic_facet |
leishmania treatment Soranjidiol photodynamic effect leishmania tratamiento Soranjidiol efecto fotodinamico |
| author |
Dimmer, JA Barrionuevo, CN Rivarola , HW Núñez Montoya, SC |
| author_facet |
Dimmer, JA Barrionuevo, CN Rivarola , HW Núñez Montoya, SC |
| author_sort |
Dimmer, JA |
| title |
Photodynamic effect of Soranjidiol on intracellular amastigotes of Leishmania braziliensis |
| title_short |
Photodynamic effect of Soranjidiol on intracellular amastigotes of Leishmania braziliensis |
| title_full |
Photodynamic effect of Soranjidiol on intracellular amastigotes of Leishmania braziliensis |
| title_fullStr |
Photodynamic effect of Soranjidiol on intracellular amastigotes of Leishmania braziliensis |
| title_full_unstemmed |
Photodynamic effect of Soranjidiol on intracellular amastigotes of Leishmania braziliensis |
| title_sort |
photodynamic effect of soranjidiol on intracellular amastigotes of leishmania braziliensis |
| description |
The study of new treatments on the amastigote form (A) of Leishmania is important, since these multiply within macrophages (M), spreading the infection within humans. Soranjidiol (Sor) is a compound (genus Heterophyllaea) that possesses photosensitizing properties. The effect of Sor on M from the J774.A1 cell line, infected with A from L. braziliensis and using white LED, was studied.
2x105 M/well were coinfected with a 1:10 MOI of promastigotes. After 24 h, the following tests were added to the microplate, protected from light (n=3): negative control (CN, only with PBS), Amphotericin B 0.5 µM (Anf), and Sor at 5 and 10 µM (non-cytotoxic Sor concentrations for M). For the irradiation conditions (n=3), the following were included: CN (CN-L) and Sor at 5 and 10 µM (Sor-5L and Sor-10L). After 10 min (pre-irradiation time), they were irradiated with LED (dose: 11 J/cm2) for 15 min. At the end, the solutions were replaced by RPMI medium, incubating them for 72 h. We performed Giemsa staining to determine the infection rate (IT): number of intracellular A/M infected multiplied by the percentage of infection. For the comparison of the treatments, a two-way ANOVA and the Bonferroni post hoc test were performed.
The RT after treatment with Sor in the dark and at both concentrations did not present significant differences with respect to CN (509.1 ± 33.5). Sor-5L photoactivation reduced RT (354.6 ± 47.6) by 27% compared to CN-L (486.3 ± 8.8, p<0.05) and was similar to that obtained with Anf (347.5 ± 38.6). For Sor-10L, a greater reduction in IT was obtained (184.9 ± 67.0, p<0.0001), which represents 62% less compared to CN-L.
In conclusion, treatment with Sor and white LED significantly reduced IT in a dose-dependent manner. These results agree with those obtained in previous trials on L. amazonensis, which motivates us to continue with the study of Sor and/or the implementation of a combination therapy with Anf, in order to achieve an effective and accessible treatment for cutaneous leishmaniasis. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2023 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/42646 |
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2024-09-03T21:04:40Z |
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I10-R327-article-426462023-10-19T21:20:25Z Photodynamic effect of Soranjidiol on intracellular amastigotes of Leishmania braziliensis Efecto fotodinámico de Soranjidiol sobre amastigotes intracelulares de Leishmania braziliensis Dimmer, JA Barrionuevo, CN Rivarola , HW Núñez Montoya, SC leishmania treatment Soranjidiol photodynamic effect leishmania tratamiento Soranjidiol efecto fotodinamico The study of new treatments on the amastigote form (A) of Leishmania is important, since these multiply within macrophages (M), spreading the infection within humans. Soranjidiol (Sor) is a compound (genus Heterophyllaea) that possesses photosensitizing properties. The effect of Sor on M from the J774.A1 cell line, infected with A from L. braziliensis and using white LED, was studied. 2x105 M/well were coinfected with a 1:10 MOI of promastigotes. After 24 h, the following tests were added to the microplate, protected from light (n=3): negative control (CN, only with PBS), Amphotericin B 0.5 µM (Anf), and Sor at 5 and 10 µM (non-cytotoxic Sor concentrations for M). For the irradiation conditions (n=3), the following were included: CN (CN-L) and Sor at 5 and 10 µM (Sor-5L and Sor-10L). After 10 min (pre-irradiation time), they were irradiated with LED (dose: 11 J/cm2) for 15 min. At the end, the solutions were replaced by RPMI medium, incubating them for 72 h. We performed Giemsa staining to determine the infection rate (IT): number of intracellular A/M infected multiplied by the percentage of infection. For the comparison of the treatments, a two-way ANOVA and the Bonferroni post hoc test were performed. The RT after treatment with Sor in the dark and at both concentrations did not present significant differences with respect to CN (509.1 ± 33.5). Sor-5L photoactivation reduced RT (354.6 ± 47.6) by 27% compared to CN-L (486.3 ± 8.8, p<0.05) and was similar to that obtained with Anf (347.5 ± 38.6). For Sor-10L, a greater reduction in IT was obtained (184.9 ± 67.0, p<0.0001), which represents 62% less compared to CN-L. In conclusion, treatment with Sor and white LED significantly reduced IT in a dose-dependent manner. These results agree with those obtained in previous trials on L. amazonensis, which motivates us to continue with the study of Sor and/or the implementation of a combination therapy with Anf, in order to achieve an effective and accessible treatment for cutaneous leishmaniasis. El estudio de nuevos tratamientos sobre la forma amastigote (A) de Leishmania es importante, ya que estas se multiplican dentro de los macrófagos (M), propagando la infección dentro del ser humano. Soranjidiol (Sor) es un compuesto (género Heterophyllaea) que posee propiedades fotosensibilizadoras. Se estudió el efecto de Sor sobre M de la línea celular J774.A1, infectados con A de L. braziliensis y empleando LED blanco. Se coinfectaron 2x105 M/pocillo con un MOI 1:10 de promastigotes. Luego de 24 h, se adicionaron a la microplaca los siguientes ensayos, resguardados de la luz (n=3): control negativo (CN, sólo con PBS), Anfotericina B 0,5 µM (Anf), y Sor a 5 y 10 µM (concentraciones de Sor no citotóxicas para M). Para las condiciones de irradiación (n=3), se incluyeron: CN (CN-L) y Sor a 5 y 10 µM (Sor-5L y Sor-10L). Luego de 10 min (tiempo de pre-irradiación), fueron irradiadas con LED (dosis: 11 J/cm2) durante 15 min. Al finalizar, las soluciones fueron reemplazadas por medio RPMI incubándolas durante 72 h. Realizamos la tinción con Giemsa para determinar la tasa de infección (TI): n° de A intracelulares/M infectados por el porcentaje de infección. Para la comparación de los tratamientos se realizó un ANOVA de dos vías y el test post hoc de Bonferroni. Las TI luego del tratamiento con Sor en oscuridad y a ambas concentraciones, no presentaron diferencias significativas respecto al CN (509,1 ± 33,5). La fotoactivación de Sor-5L redujo la TI (354,6 ± 47,6) en un 27 % respecto al CN-L (486,3 ± 8,8, p<0,05) y fue similar a la que se obtiene con Anf (347,5 ± 38,6). Para Sor-10L se obtuvo una mayor reducción de la TI (184,9 ± 67,0, p<0,0001), que representa un 62 % menos respecto al CN-L. En conclusión, el tratamiento con Sor y LED blanco redujo significativamente la TI de manera dosis dependiente. Estos resultados concuerdan con los obtenidos en ensayos previos sobre L. amazonensis, lo cual motiva a continuar con el estudio de Sor y/o la implementación de una terapia combinada con Anf, a fin de lograr un tratamiento efectivo y accesible para la leishmaniasis cutanea. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-10-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/42646 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 (2023): Suplemento JIC XXIV Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 (2023): Suplemento JIC XXIV Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 (2023): Suplemento JIC XXIV 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/42646/42851 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0 |