Detection of Human Parvovirus B19 in peripheral blood cells
Human parvovirus B19/B19V is characterized by its tropism towards erythroid progenitor cells present in the bone marrow and, to a lesser extent, in the peripheral blood mononuclear cell fraction (proerythroblasts and reticulocytes). In B19V infection, viremia reaches high titers 7-10 days after infe...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2023
|
| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/42600 |
| Aporte de: |
| Sumario: | Human parvovirus B19/B19V is characterized by its tropism towards erythroid progenitor cells present in the bone marrow and, to a lesser extent, in the peripheral blood mononuclear cell fraction (proerythroblasts and reticulocytes). In B19V infection, viremia reaches high titers 7-10 days after infection, followed by the elevation of specific antibody levels one or two weeks later. The antibodies control the infection, while viral DNA can be detected in circulation for a few weeks or months. The diagnosis is relevant in situations of immunosuppression, underlying hematological diseases, and in pregnant women. Primary infection during gestation can be transmitted to the fetus and cause placental and/or fetal-neonatal conditions related to anemia and inflammation, even without maternal symptoms. The diagnosis is usually made by determining specific antibodies, and it is useful to complement with viral DNA detection. The aim of this study is to report the detection of B19V from peripheral blood mononuclear cells in a case with suspected infection related to spontaneous abortion.
In blood/serum samples, anti-B19V IgM and IgG were determined using ELISA (Ridascreen R-Biopharm). Nucleic acids were extracted from both serum and peripheral blood mononuclear cells, free of red blood cells, to then determine viral DNA through endpoint PCR with primers targeting the NS1 region.
As part of the differential diagnosis, immune, cardiac, and genetic causes were ruled out, as well as infections by Herpes Simplex 1 and 2 viruses, Epstein Barr virus, Cytomegalovirus, and Chlamydia trachomatis. Serology for B19V yielded the following results: IgM 29 IU/mL (cut-off value 12 IU/mL); IgG 5.3 IU/mL (cut-off value 5 IU/mL). Viral DNA was only detected in the peripheral blood mononuclear cell fraction.
These data indicate recent acute infection by B19V (IgM+ and presence of the virus in blood cells), suggesting the remission phase at the same time (absence of free virus in serum). In conclusion, B19V infection was detected in a case of spontaneous abortion, confirming the presence of the virus in peripheral blood mononuclear cells. |
|---|