Detection of Human Parvovirus B19 in peripheral blood cells

Human parvovirus B19/B19V is characterized by its tropism towards erythroid progenitor cells present in the bone marrow and, to a lesser extent, in the peripheral blood mononuclear cell fraction (proerythroblasts and reticulocytes). In B19V infection, viremia reaches high titers 7-10 days after infe...

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Autores principales: Colazo Salbetti , MB, Riberi , MI, Tenaglia , M, Hernández Fregonese, G, Alfaro , J, Bertoldi , A, Isa , MB, Adamo , MP
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023
Materias:
parvovirus b19
Diagnosis
serology
viremia
PCR
Diagnóstico
serología
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/42600
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
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institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic parvovirus b19
Diagnosis
serology
viremia
PCR
parvovirus b19
Diagnóstico
serología
viremia
PCR
spellingShingle parvovirus b19
Diagnosis
serology
viremia
PCR
parvovirus b19
Diagnóstico
serología
viremia
PCR
Colazo Salbetti , MB
Riberi , MI
Tenaglia , M
Hernández Fregonese, G,
Alfaro , J
Bertoldi , A
Isa , MB
Adamo , MP
Detection of Human Parvovirus B19 in peripheral blood cells
topic_facet parvovirus b19
Diagnosis
serology
viremia
PCR
parvovirus b19
Diagnóstico
serología
viremia
PCR
author Colazo Salbetti , MB
Riberi , MI
Tenaglia , M
Hernández Fregonese, G,
Alfaro , J
Bertoldi , A
Isa , MB
Adamo , MP
author_facet Colazo Salbetti , MB
Riberi , MI
Tenaglia , M
Hernández Fregonese, G,
Alfaro , J
Bertoldi , A
Isa , MB
Adamo , MP
author_sort Colazo Salbetti , MB
title Detection of Human Parvovirus B19 in peripheral blood cells
title_short Detection of Human Parvovirus B19 in peripheral blood cells
title_full Detection of Human Parvovirus B19 in peripheral blood cells
title_fullStr Detection of Human Parvovirus B19 in peripheral blood cells
title_full_unstemmed Detection of Human Parvovirus B19 in peripheral blood cells
title_sort detection of human parvovirus b19 in peripheral blood cells
description Human parvovirus B19/B19V is characterized by its tropism towards erythroid progenitor cells present in the bone marrow and, to a lesser extent, in the peripheral blood mononuclear cell fraction (proerythroblasts and reticulocytes). In B19V infection, viremia reaches high titers 7-10 days after infection, followed by the elevation of specific antibody levels one or two weeks later. The antibodies control the infection, while viral DNA can be detected in circulation for a few weeks or months. The diagnosis is relevant in situations of immunosuppression, underlying hematological diseases, and in pregnant women. Primary infection during gestation can be transmitted to the fetus and cause placental and/or fetal-neonatal conditions related to anemia and inflammation, even without maternal symptoms. The diagnosis is usually made by determining specific antibodies, and it is useful to complement with viral DNA detection. The aim of this study is to report the detection of B19V from peripheral blood mononuclear cells in a case with suspected infection related to spontaneous abortion. In blood/serum samples, anti-B19V IgM and IgG were determined using ELISA (Ridascreen R-Biopharm). Nucleic acids were extracted from both serum and peripheral blood mononuclear cells, free of red blood cells, to then determine viral DNA through endpoint PCR with primers targeting the NS1 region. As part of the differential diagnosis, immune, cardiac, and genetic causes were ruled out, as well as infections by Herpes Simplex 1 and 2 viruses, Epstein Barr virus, Cytomegalovirus, and Chlamydia trachomatis. Serology for B19V yielded the following results: IgM 29 IU/mL (cut-off value 12 IU/mL); IgG 5.3 IU/mL (cut-off value 5 IU/mL). Viral DNA was only detected in the peripheral blood mononuclear cell fraction. These data indicate recent acute infection by B19V (IgM+ and presence of the virus in blood cells), suggesting the remission phase at the same time (absence of free virus in serum). In conclusion, B19V infection was detected in a case of spontaneous abortion, confirming the presence of the virus in peripheral blood mononuclear cells.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2023
url https://revistas.unc.edu.ar/index.php/med/article/view/42600
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first_indexed 2024-09-03T21:04:38Z
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spelling I10-R327-article-426002023-10-19T21:20:33Z Detection of Human Parvovirus B19 in peripheral blood cells Detección de Parvovirus humano B19 en células de sangre periférica Colazo Salbetti , MB Riberi , MI Tenaglia , M Hernández Fregonese, G, Alfaro , J Bertoldi , A Isa , MB Adamo , MP parvovirus b19 Diagnosis serology viremia PCR parvovirus b19 Diagnóstico serología viremia PCR Human parvovirus B19/B19V is characterized by its tropism towards erythroid progenitor cells present in the bone marrow and, to a lesser extent, in the peripheral blood mononuclear cell fraction (proerythroblasts and reticulocytes). In B19V infection, viremia reaches high titers 7-10 days after infection, followed by the elevation of specific antibody levels one or two weeks later. The antibodies control the infection, while viral DNA can be detected in circulation for a few weeks or months. The diagnosis is relevant in situations of immunosuppression, underlying hematological diseases, and in pregnant women. Primary infection during gestation can be transmitted to the fetus and cause placental and/or fetal-neonatal conditions related to anemia and inflammation, even without maternal symptoms. The diagnosis is usually made by determining specific antibodies, and it is useful to complement with viral DNA detection. The aim of this study is to report the detection of B19V from peripheral blood mononuclear cells in a case with suspected infection related to spontaneous abortion. In blood/serum samples, anti-B19V IgM and IgG were determined using ELISA (Ridascreen R-Biopharm). Nucleic acids were extracted from both serum and peripheral blood mononuclear cells, free of red blood cells, to then determine viral DNA through endpoint PCR with primers targeting the NS1 region. As part of the differential diagnosis, immune, cardiac, and genetic causes were ruled out, as well as infections by Herpes Simplex 1 and 2 viruses, Epstein Barr virus, Cytomegalovirus, and Chlamydia trachomatis. Serology for B19V yielded the following results: IgM 29 IU/mL (cut-off value 12 IU/mL); IgG 5.3 IU/mL (cut-off value 5 IU/mL). Viral DNA was only detected in the peripheral blood mononuclear cell fraction. These data indicate recent acute infection by B19V (IgM+ and presence of the virus in blood cells), suggesting the remission phase at the same time (absence of free virus in serum). In conclusion, B19V infection was detected in a case of spontaneous abortion, confirming the presence of the virus in peripheral blood mononuclear cells. El parvovirus humano B19/B19V se caracteriza por su tropismo hacia células progenitoras eritroides presentes en la médula ósea y, en menor proporción, en la fracción de células mononucleares de sangre periférica (proeritroblastos y reticulocitos). En la infección por B19V la viremia alcanza altos títulos 7-10 días luego del contagio, seguida por la elevación de los niveles de anticuerpos específicos una o dos semanas después. Los anticuerpos controlan la infección, mientras el ADN viral puede detectarse en circulación durante algunas semanas o meses. El diagnóstico es relevante en situaciones de inmunosupresión, enfermedades hematológicas de base y en la embarazada; en ella la primoinfección puede transmitirse al feto y ocasionar cuadros placentarios y/o fetoneonatales relacionados a anemia e inflamación, incluso sin síntomas maternos. El diagnóstico se realiza usualmente mediante determinación de anticuerpos específicos, siendo útil complementar con la detección de ADN viral. El objetivo del trabajo es reportar la detección de B19V a partir de células mononucleares de sangre periférica en un caso con sospecha de infección relacionada a aborto espontáneo. En muestra de sangre/suero se determinaron IgM e IgG anti-B19V mediante ELISA (Ridascreen R-Biopharm). Se extrajeron ácidos nucleicos tanto a partir de suero como de células mononucleares de sangre periférica libre de glóbulos rojos, para luego determinar ADN viral por PCR de punto final con primers dirigidos a la región NS1. Como parte del diagnóstico diferencial se descartaron causas inmunológicas, cardíacas y genéticas, además de infecciones por virus Herpes Simple 1 y 2, Epstein Barr, Citomegalovirus y Chlamydia trachomatis. La serología para B19V arrojó los siguientes resultados: IgM 29 UI/mL (valor de corte 12 UI/mL); IgG 5.3 UI/mL (valor de corte 5 UI/mL). Se detectó el ADN viral sólo en la fracción de células mononucleares de sangre periférica. Estos datos indican infección aguda reciente por B19V (IgM+ y presencia del virus en células sanguíneas), sugiriendo al mismo tiempo la fase de remisión (ausencia de virus libre en suero). En conclusión, se detectó infección por B19V en un caso de aborto espontáneo, confirmando la presencia del virus en células mononucleares de sangre periférica. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-10-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/42600 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 (2023): Suplemento JIC XXIV Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 (2023): Suplemento JIC XXIV Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 (2023): Suplemento JIC XXIV 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/42600/42849 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0

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