Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide
This article has been updated due to an inadvertent error and an erratum will be published in the forthcoming issue 81 (4) 2024. The doi http://dx.doi.org/10.31053/1853.0605.v80.n4.40343 does not correspond to this article. Correct DOI: http://dx.doi.org/10.31053/1853.0605.v80.n4.40930 OBJECTIVE:...
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| Autores principales: | , , , , , , , , |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2023
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/40930 |
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I10-R327-article-40930 |
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Universidad Nacional de Córdoba |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
melatonin cyclophosphamide submandibular gland Wistar rats melatonina ciclofosfamida glándula submandibular ratas wistar español melatonina ciclofosfamida glândula submandibular ratos wistar |
| spellingShingle |
melatonin cyclophosphamide submandibular gland Wistar rats melatonina ciclofosfamida glándula submandibular ratas wistar español melatonina ciclofosfamida glândula submandibular ratos wistar Bachmeier, Evelin Wietz , Fernando Martin Porta, Daniela Josefina Moine, Lorena Dubersarsky, Claudio Gastón Francia, Catalina Melchora Samar, Maria Elena Rivoira, Maria Angelica Mazzeo, Marcelo Adrian Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide |
| topic_facet |
melatonin cyclophosphamide submandibular gland Wistar rats melatonina ciclofosfamida glándula submandibular ratas wistar español melatonina ciclofosfamida glândula submandibular ratos wistar |
| author |
Bachmeier, Evelin Wietz , Fernando Martin Porta, Daniela Josefina Moine, Lorena Dubersarsky, Claudio Gastón Francia, Catalina Melchora Samar, Maria Elena Rivoira, Maria Angelica Mazzeo, Marcelo Adrian |
| author_facet |
Bachmeier, Evelin Wietz , Fernando Martin Porta, Daniela Josefina Moine, Lorena Dubersarsky, Claudio Gastón Francia, Catalina Melchora Samar, Maria Elena Rivoira, Maria Angelica Mazzeo, Marcelo Adrian |
| author_sort |
Bachmeier, Evelin |
| title |
Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide |
| title_short |
Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide |
| title_full |
Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide |
| title_fullStr |
Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide |
| title_full_unstemmed |
Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide |
| title_sort |
melatonin reverses oxidative damage in the submandibular gland of rats treated with cyclophosphamide |
| description |
This article has been updated due to an inadvertent error and an erratum will be published in the forthcoming issue 81 (4) 2024.
The doi http://dx.doi.org/10.31053/1853.0605.v80.n4.40343 does not correspond to this article.
Correct DOI: http://dx.doi.org/10.31053/1853.0605.v80.n4.40930
OBJECTIVE: Cyclophosphamide (Cf) produces oxidative damage in rat submandibular gland (GSM). In the present work we evaluated the antioxidant protective effect of melatonin (MLT) in GSM of rats treated with Cf.
METHODS: 40 adult male Wistar rats were divided into 5 groups (G): G1: control; G2: Control+Ethanol: treated with 1% ethanol for 10 consecutive days. On days 11 and 12 they received a dose of saline; G3: Cf: treated with 1% ethanol for 12 days, days 11 and 12 they received an intraperitoneal (i.p.) dose of Cf 50 mg/Kg/kg of saline. ) of Cf 50 mg/kg bw; G4: Cf + MLT: MLT (5 mg/kg bw, intraperitoneal, dissolved in 1% ethanol) was administered daily, days 11 and 12 received Cf same as G3; G5: MLT: treated 12 consecutive days with MLT (same dose as G4). Animals were anaesthetised, both GSM were removed and sacrificed, after 24 h fasting. Uric acid (UA), lipid peroxides (LPs) and aqueous peroxides (APs) and superoxide dismutase (SOD) activity were measured in GSM homogenate. Statistical analysis: ANOVA and Bonferroni test, considering significant p<0.05.
RESULTS: Cf treatment decreased AU concentration and SOD activity (AU, mg/mg prot., G1: 2.50±0.68; G2: 2.18±0.13; G3: 0.54±0.09* G4: 1.95±0.24#, G5: 2.64±0.47, *p<0.01 G3 vs G1, G2, G4; #p<0.01 G4 vs G3 and G5; SOD, U/mg prot, G1: 4.57±0.95, G2: 4.79±0.94, G3: 2.18±0.53*, G4: 5.13±1.10, G5: 5.09±0.39, *p< 0.01 G3 vs G1, G2, G4 and G5). MLT treatment prevented these effects. In addition, Cf increased PL and PA formation.
CONCLUSION: MLT improved the redox status in GSM of Cf-treated rats. MLT could prevent oxidative processes in GSM produced by Cf. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2023 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/40930 |
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2024-09-03T21:04:30Z |
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I10-R327-article-409302024-11-01T15:11:18Z Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide Melatonina revierte el daño oxidativo en glándula submandibular de ratas tratadas con Ciclofosfamida A melatonina reverte os danos oxidativos na glândula submandibular de ratos tratados com Ciclofosfamida Bachmeier, Evelin Wietz , Fernando Martin Porta, Daniela Josefina Moine, Lorena Dubersarsky, Claudio Gastón Francia, Catalina Melchora Samar, Maria Elena Rivoira, Maria Angelica Mazzeo, Marcelo Adrian melatonin cyclophosphamide submandibular gland Wistar rats melatonina ciclofosfamida glándula submandibular ratas wistar español melatonina ciclofosfamida glândula submandibular ratos wistar This article has been updated due to an inadvertent error and an erratum will be published in the forthcoming issue 81 (4) 2024. The doi http://dx.doi.org/10.31053/1853.0605.v80.n4.40343 does not correspond to this article. Correct DOI: http://dx.doi.org/10.31053/1853.0605.v80.n4.40930 OBJECTIVE: Cyclophosphamide (Cf) produces oxidative damage in rat submandibular gland (GSM). In the present work we evaluated the antioxidant protective effect of melatonin (MLT) in GSM of rats treated with Cf. METHODS: 40 adult male Wistar rats were divided into 5 groups (G): G1: control; G2: Control+Ethanol: treated with 1% ethanol for 10 consecutive days. On days 11 and 12 they received a dose of saline; G3: Cf: treated with 1% ethanol for 12 days, days 11 and 12 they received an intraperitoneal (i.p.) dose of Cf 50 mg/Kg/kg of saline. ) of Cf 50 mg/kg bw; G4: Cf + MLT: MLT (5 mg/kg bw, intraperitoneal, dissolved in 1% ethanol) was administered daily, days 11 and 12 received Cf same as G3; G5: MLT: treated 12 consecutive days with MLT (same dose as G4). Animals were anaesthetised, both GSM were removed and sacrificed, after 24 h fasting. Uric acid (UA), lipid peroxides (LPs) and aqueous peroxides (APs) and superoxide dismutase (SOD) activity were measured in GSM homogenate. Statistical analysis: ANOVA and Bonferroni test, considering significant p<0.05. RESULTS: Cf treatment decreased AU concentration and SOD activity (AU, mg/mg prot., G1: 2.50±0.68; G2: 2.18±0.13; G3: 0.54±0.09* G4: 1.95±0.24#, G5: 2.64±0.47, *p<0.01 G3 vs G1, G2, G4; #p<0.01 G4 vs G3 and G5; SOD, U/mg prot, G1: 4.57±0.95, G2: 4.79±0.94, G3: 2.18±0.53*, G4: 5.13±1.10, G5: 5.09±0.39, *p< 0.01 G3 vs G1, G2, G4 and G5). MLT treatment prevented these effects. In addition, Cf increased PL and PA formation. CONCLUSION: MLT improved the redox status in GSM of Cf-treated rats. MLT could prevent oxidative processes in GSM produced by Cf. Este artículo ha sido actualizado por error involuntario y será publicado la fe de errata en el próximo número 81 (4) 2024. El doi http://dx.doi.org/10.31053/1853.0605.v80.n4.40343 no corresponde al presente artículo. DOI correcto: http://dx.doi.org/10.31053/1853.0605.v80.n4.40930 OBJETIVO: Ciclofosfamida (Cf) produce daño oxidativo en glándula submandibular (GSM) de ratas. En el presente trabajo se evaluó el efecto protector antioxidante de melatonina (MLT) en GSM de ratas tratadas con Cf. METODO: Se utilizaron 40 ratas Wistar machos adultas divididas en 5 grupos (G): G1: control; G2: Control+Etanol: tratados con etanol al 1% durante 10 días consecutivos. Los días 11 y 12 recibieron una dosis de solución salina; G3: Cf: tratados con etanol al 1% durante 12 días, días 11 y 12 recibieron una dosis intraperitoneal (i.p.) de Cf de 50 mg/Kg de pc; G4: Cf + MLT: se administró diariamente MLT (5 mg/Kg pc, intraperitoneal, disuelta en etanol al 1%), días 11 y 12 recibieron Cf igual que G3; G5: MLT: tratamiento 12 días consecutivos con MLT (igual dosis de G4). Los animales fueron anestesiados, extirpándose ambas GSM y sacrificados, previo ayuno 24 hs. Se midió la concentración de ácido úrico (AU), peróxidos lipídicos (PL) y acuosos (PA) y actividad de superóxido dismutasa (SOD) en homogenato de GSM. Análisis estadístico: ANOVA y test de Bonferroni, considerando significativo p<0,05. RESULTADOS: El tratamiento con Cf disminuyó la concentración de AU y la actividad de SOD (AU, mg/mg prot., G1: 2,50±0,68; G2: 2,18±0,13; G3: 0,54±0,09* G4: 1,95±0,24#, G5: 2,64±0,47, *p< 0,01 G3 vs G1, G2, G4; #p< 0,01 G4 vs G3 y G5; SOD, U/mg prot., G1: 4,57±0.95, G2: 4,79±0,94, G3: 2,18±0,53*, G4: 5,13±1,10, G5: 5,09±0,39, *p< 0,01 G3 vs G1, G2, G4 y G5). El tratamiento con MLT previno esos efectos. Además, Cf aumentó la formación PL y PA. CONCLUSION: MLT mejoró el estado redox en GSM de ratas tratadas con Cf. MLT podría prevenir los procesos oxidativos en GSM producidos por Cf. Este artigo foi atualizado devido a um erro inadvertido e será publicada uma errata na próxima edição 81 (4) 2024. O doi http://dx.doi.org/10.31053/1853.0605.v80.n4.40343 não corresponde a este artigo. Correct DOI: http://dx.doi.org/10.31053/1853.0605.v80.n4.40930 OBJETIVO: Ciclofosfamida (Cf) produz danos oxidativos na glândula submandibular de rato (GSM). No presente trabalho avaliamos o efeito protector antioxidante da melatonina (MLT) no GSM de ratos tratados com Cf. MÉTODOS: 40 ratos Wistar machos adultos foram divididos em 5 grupos (G): G1: controlo; G2: controlo+Etanol: tratado com 1% de etanol durante 10 dias consecutivos. Nos dias 11 e 12 receberam uma dose de soro; G3: Cf: tratado com 1% de etanol durante 12 dias, dias 11 e 12 receberam uma dose intraperitoneal (i.p.) de Cf 50 mg/Kg/kg de soro fisiológico de Cf 50 mg/kg pb; G4: Cf + MLT: MLT (5 mg/kg pb, intraperitoneal, dissolvido em 1% de etanol) foi administrado diariamente, os dias 11 e 12 receberam Cf igual a G3; G5: MLT: tratado 12 dias consecutivos com MLT (mesma dose que G4). Os animais foram anestesiados, ambos os GSM foram removidos e sacrificados, após jejum de 24 h. A actividade de ácido úrico (UA), peróxidos lipídicos (LPs) e peróxidos aquosos (APs) e superóxido dismutase (SOD) foram medidos na homogeneização do GSM. Análise estatística: teste de ANOVA e Bonferroni, considerando p<0,05 significativo. RESULTADOS: O tratamento Cf diminuiu a concentração AU e a actividade de SOD (AU, mg/mg prot., G1: 2,50±0,68; G2: 2,18±0,13; G3: 0,54±0,09* G4: 1,95±0,24#, G5: 2,64±0,47, *p<0,01 G3 vs G1, G2, G4; #p<0,01 G4 vs G3 e G5; SOD, U/mg prot, G1: 4,57±0,95, G2: 4,79±0,94, G3: 2,18±0,53*, G4: 5,13±1,10, G5: 5,09±0,39,*p< 0,01 G3 vs G1, G2, G4 e G5). O tratamento MLT impediu estes efeitos. Além disso, Cf aumentou a formação de PL e PA. CONCLUSÃO: O MLT melhorou o estado redox no GSM de ratos tratados com Cf. O MLT poderia prevenir processos oxidativos no GSM produzido por Cf. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-12-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto application/pdf text/html application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/40930 10.31053/1853.0605.v80.n4.40930 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 No. 4 (2023): Publicaciones especiales por 80° Aniversario; 404-419 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 Núm. 4 (2023): Publicaciones especiales por 80° Aniversario; 404-419 Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 n. 4 (2023): Publicaciones especiales por 80° Aniversario; 404-419 1853-0605 0014-6722 10.31053/1853.0605.v80.n4 spa https://revistas.unc.edu.ar/index.php/med/article/view/40930/44177 https://revistas.unc.edu.ar/index.php/med/article/view/40930/43186 https://revistas.unc.edu.ar/index.php/med/article/view/40930/47048 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0 |