Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes
Type 2 diabetes mellitus (DM2) has underlying pathophysiological processes of low-grade systemic inflammation and oxidative stress that produce metabolic alterations and systemic damage. The harm to the central nervous system (CNS) by these pathophysiological processes has been poorly studied. Expos...
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| Autores principales: | , , , , , |
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39087 |
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| Sumario: | Type 2 diabetes mellitus (DM2) has underlying pathophysiological processes of low-grade systemic inflammation and oxidative stress that produce metabolic alterations and systemic damage. The harm to the central nervous system (CNS) by these pathophysiological processes has been poorly studied. Exposure to an enriched environment that allows greater physical activity and has more varied stimuli can reduce or prevent the deleterious effects produced on the CNS by various pathologies. Objective: to study the effect of an enriched environment on oxidative stress in the CNS in a rat model with DM2.
60 adult male Wistar rats (12 months), received food and water ad libitum for 16 months. The problem groups were fed a high-fat diet (HFD, 30% total, 25% saturated) and moderate alcohol consumption (Alc, 0.42 g/kg body weight/day). The enriched environment (AE) consists of a larger cage with wheels, platforms, ramps, etc. Five experimental groups were used: HFD; Alk; HFD+Alc; HFD+Alc+AE and Control (Chow diet). Blood glucose, HbA1c, peroxides (hydro and lipo), nitrites, and gamma-glutamyltranspeptidase (GGT) activity in blood and brain were analyzed. Data were statistically analyzed by ANOVA (α=0.05).
All the test animals became diabetic. Oxidative stress is increased in animals with HFD diet and to a lesser extent in those with alcohol consumption. Plasma GGT activity increased in the problem groups and exposure to EO was able to reduce it by 46% compared to the Control (p=0.05). In brain tissue, peroxides (hydro and lipo) increased in the problem groups (16 to 53%) and in the group with EA, lipoperoxides decreased to levels similar to the Control (p=0.05). In hydroperoxides, a decrease was also observed, but not significant. The levels of nitrites in brain tissue only increased (900%) in the HFD group, the other groups recorded lower values (90 to 92%) than the controls (p=0.05).
Oxidative stress in the CNS is increased in a model of diabetic rats fed a high-fat diet and moderate alcohol consumption, and exposure to an enriched environment decreased the levels of the markers studied. |
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