Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes
Type 2 diabetes mellitus (DM2) has underlying pathophysiological processes of low-grade systemic inflammation and oxidative stress that produce metabolic alterations and systemic damage. The harm to the central nervous system (CNS) by these pathophysiological processes has been poorly studied. Expos...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39087 |
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I10-R327-article-39087 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
enriched environment oxidative stress Type 2 Diabetes Mellitus brain high fat diet ambiente enriquecido estrés oxidativo Diabetes Mellitus Tipo 2 cerebro Dieta Rica en Grasas . |
| spellingShingle |
enriched environment oxidative stress Type 2 Diabetes Mellitus brain high fat diet ambiente enriquecido estrés oxidativo Diabetes Mellitus Tipo 2 cerebro Dieta Rica en Grasas . Díaz, M Díaz, GT Priotto, S López , C Pigino , G Repossi, G Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes |
| topic_facet |
enriched environment oxidative stress Type 2 Diabetes Mellitus brain high fat diet ambiente enriquecido estrés oxidativo Diabetes Mellitus Tipo 2 cerebro Dieta Rica en Grasas . |
| author |
Díaz, M Díaz, GT Priotto, S López , C Pigino , G Repossi, G |
| author_facet |
Díaz, M Díaz, GT Priotto, S López , C Pigino , G Repossi, G |
| author_sort |
Díaz, M |
| title |
Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes |
| title_short |
Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes |
| title_full |
Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes |
| title_fullStr |
Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes |
| title_full_unstemmed |
Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes |
| title_sort |
effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes |
| description |
Type 2 diabetes mellitus (DM2) has underlying pathophysiological processes of low-grade systemic inflammation and oxidative stress that produce metabolic alterations and systemic damage. The harm to the central nervous system (CNS) by these pathophysiological processes has been poorly studied. Exposure to an enriched environment that allows greater physical activity and has more varied stimuli can reduce or prevent the deleterious effects produced on the CNS by various pathologies. Objective: to study the effect of an enriched environment on oxidative stress in the CNS in a rat model with DM2.
60 adult male Wistar rats (12 months), received food and water ad libitum for 16 months. The problem groups were fed a high-fat diet (HFD, 30% total, 25% saturated) and moderate alcohol consumption (Alc, 0.42 g/kg body weight/day). The enriched environment (AE) consists of a larger cage with wheels, platforms, ramps, etc. Five experimental groups were used: HFD; Alk; HFD+Alc; HFD+Alc+AE and Control (Chow diet). Blood glucose, HbA1c, peroxides (hydro and lipo), nitrites, and gamma-glutamyltranspeptidase (GGT) activity in blood and brain were analyzed. Data were statistically analyzed by ANOVA (α=0.05).
All the test animals became diabetic. Oxidative stress is increased in animals with HFD diet and to a lesser extent in those with alcohol consumption. Plasma GGT activity increased in the problem groups and exposure to EO was able to reduce it by 46% compared to the Control (p=0.05). In brain tissue, peroxides (hydro and lipo) increased in the problem groups (16 to 53%) and in the group with EA, lipoperoxides decreased to levels similar to the Control (p=0.05). In hydroperoxides, a decrease was also observed, but not significant. The levels of nitrites in brain tissue only increased (900%) in the HFD group, the other groups recorded lower values (90 to 92%) than the controls (p=0.05).
Oxidative stress in the CNS is increased in a model of diabetic rats fed a high-fat diet and moderate alcohol consumption, and exposure to an enriched environment decreased the levels of the markers studied. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39087 |
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2024-09-03T21:04:10Z |
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I10-R327-article-390872024-04-15T16:14:45Z Effect of exposure to an enriched environment on brain oxidative stress markers in an experimental model of type 2 diabetes Efecto de la exposición a un ambiente enriquecido sobre marcadores de estrés oxidativo en cerebro en un modelo experimental de diabetes tipo 2 . Díaz, M Díaz, GT Priotto, S López , C Pigino , G Repossi, G enriched environment oxidative stress Type 2 Diabetes Mellitus brain high fat diet ambiente enriquecido estrés oxidativo Diabetes Mellitus Tipo 2 cerebro Dieta Rica en Grasas . Type 2 diabetes mellitus (DM2) has underlying pathophysiological processes of low-grade systemic inflammation and oxidative stress that produce metabolic alterations and systemic damage. The harm to the central nervous system (CNS) by these pathophysiological processes has been poorly studied. Exposure to an enriched environment that allows greater physical activity and has more varied stimuli can reduce or prevent the deleterious effects produced on the CNS by various pathologies. Objective: to study the effect of an enriched environment on oxidative stress in the CNS in a rat model with DM2. 60 adult male Wistar rats (12 months), received food and water ad libitum for 16 months. The problem groups were fed a high-fat diet (HFD, 30% total, 25% saturated) and moderate alcohol consumption (Alc, 0.42 g/kg body weight/day). The enriched environment (AE) consists of a larger cage with wheels, platforms, ramps, etc. Five experimental groups were used: HFD; Alk; HFD+Alc; HFD+Alc+AE and Control (Chow diet). Blood glucose, HbA1c, peroxides (hydro and lipo), nitrites, and gamma-glutamyltranspeptidase (GGT) activity in blood and brain were analyzed. Data were statistically analyzed by ANOVA (α=0.05). All the test animals became diabetic. Oxidative stress is increased in animals with HFD diet and to a lesser extent in those with alcohol consumption. Plasma GGT activity increased in the problem groups and exposure to EO was able to reduce it by 46% compared to the Control (p=0.05). In brain tissue, peroxides (hydro and lipo) increased in the problem groups (16 to 53%) and in the group with EA, lipoperoxides decreased to levels similar to the Control (p=0.05). In hydroperoxides, a decrease was also observed, but not significant. The levels of nitrites in brain tissue only increased (900%) in the HFD group, the other groups recorded lower values (90 to 92%) than the controls (p=0.05). Oxidative stress in the CNS is increased in a model of diabetic rats fed a high-fat diet and moderate alcohol consumption, and exposure to an enriched environment decreased the levels of the markers studied. La diabetes mellitus tipo 2 (DM2) posee procesos fisiopatológicos subyacentes de inflamación sistémica de bajo grado y estrés oxidativo que producen alteraciones metabólicas y daño sistémico. La afectación del sistema nervioso central (SNC) por estos procesos fisiopatológicos ha sido escasamente estudiada. La exposición a un ambiente enriquecido que permite mayor actividad física y posee estímulos más variados puede disminuir o prevenir los efectos deletéreos producidos sobre el SNC por varias patologías. Objetivo: estudiar el efecto de un ambiente enriquecido sobre el estrés oxidativo en el SNC en un modelo de ratas con DM2. Se utilizaron 60 ratas Wistar macho adultas (12 meses), que recibieron comida y agua ad libitum durante 16 meses. Los grupos problema fueron alimentados con una dieta rica en grasas (HFD, 30% total, 25%saturadas) y consumo moderado de alcohol (Alc, 0,42 g/kg de peso/día). El ambiente enriquecido (AE) consta de una jaula mayor tamaño con ruedas, plataformas, rampas, etc. Se utilizaron 5 grupos experimentales: HFD; Alc; HFD+Alc; HFD+Alc+AE y Control (dieta Chow). Se analizaron glucemias, HbA1c, peróxidos (hidro y lipo), nitritos y actividad gamaglutamiltranspeptidasa (GGT) en sangre y cerebro. Los datos fueron analizados estadísticamente mediante ANOVA (α=0,05). Todos los animales problema se volvieron diabéticos. El estrés oxidativo está aumentado en animales con dieta HFD y en menor medida en aquellos con consumo de alcohol. La actividad GGT en plasma aumentó en los grupos problema y la exposición al AE logró disminuirla un 46% respecto al Control (p=0,05). En tejido cerebral los peróxidos (hidro y lipo) aumentaron en los grupos problema (16 a 53%) y en el grupo con AE los lipoperóxidos disminuyeron a niveles similares al Control (p=0,05). En hidroperóxidos también se observó una disminución, pero no significativa. Los niveles de nitritos en tejido cerebral sólo aumentaron (900%) en el grupo HFD, los demás grupos registraron valores menores (90 a 92%) que los controles (p=0,05). El estrés oxidativo en SNC está aumentado de un modelo de ratas diabéticas alimentadas con dieta rica en grasas y consumo moderado de alcohol y la exposición a un ambiente enriquecido disminuyó los niveles de los marcadores estudiados. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto . https://revistas.unc.edu.ar/index.php/med/article/view/39087 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |