Monitoring of hepatitis A virus in wastewater as an environmental surveillance tool in Córdoba, Argentina
Due to hepatitis A virus (HAV) is a fecal-oral transmission pathogen, urban wastewater surveillance is considered a key tool to track the viral circulation trends over time in the population. The aim of this study was to assess HAV detection in wastewater samples from the city of Córdoba, Argentina,...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39086 |
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I10-R327-article-39086 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
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I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
ENVIRONMENTAL SURVEILLANCE HAV wastewater vigilancia ambiental HAV aguas residuales . |
| spellingShingle |
ENVIRONMENTAL SURVEILLANCE HAV wastewater vigilancia ambiental HAV aguas residuales . Fantilli , A Di Cola, G Castro , G Barbás , MG Nates , S Masachessi, G Pisano , MB Re, VE Monitoring of hepatitis A virus in wastewater as an environmental surveillance tool in Córdoba, Argentina |
| topic_facet |
ENVIRONMENTAL SURVEILLANCE HAV wastewater vigilancia ambiental HAV aguas residuales . |
| author |
Fantilli , A Di Cola, G Castro , G Barbás , MG Nates , S Masachessi, G Pisano , MB Re, VE |
| author_facet |
Fantilli , A Di Cola, G Castro , G Barbás , MG Nates , S Masachessi, G Pisano , MB Re, VE |
| author_sort |
Fantilli , A |
| title |
Monitoring of hepatitis A virus in wastewater as an environmental surveillance tool in Córdoba, Argentina |
| title_short |
Monitoring of hepatitis A virus in wastewater as an environmental surveillance tool in Córdoba, Argentina |
| title_full |
Monitoring of hepatitis A virus in wastewater as an environmental surveillance tool in Córdoba, Argentina |
| title_fullStr |
Monitoring of hepatitis A virus in wastewater as an environmental surveillance tool in Córdoba, Argentina |
| title_full_unstemmed |
Monitoring of hepatitis A virus in wastewater as an environmental surveillance tool in Córdoba, Argentina |
| title_sort |
monitoring of hepatitis a virus in wastewater as an environmental surveillance tool in córdoba, argentina |
| description |
Due to hepatitis A virus (HAV) is a fecal-oral transmission pathogen, urban wastewater surveillance is considered a key tool to track the viral circulation trends over time in the population. The aim of this study was to assess HAV detection in wastewater samples from the city of Córdoba, Argentina, and to compare the results with the confirmed cases of hepatitis A illness reported during the same period.
Sewage samples were weekly collected from May 2020 to July 2021, from “Bajo Grande”, one of the wastewater treatment plants located in Córdoba city. Furthermore, sewage collectors of 7 neighborhoods in Córdoba city were weekly sampled from January 2021 to July 2022. Virus concentration was carried out using PEG6000 and NaCl, and automated RNA extraction and HAV molecular detection by RT-qPCR were performed afterward. Finally, the dataset on the total number of confirmed HAV infected cases reported during the same period was requested to the Epidemiology area of the Government of the Province.
Out of 748 samples analyzed, 49 were RNA-HAV+ (6.55%). One belonged to 2020 (2,04%), 8 to 2021 (16,3%), and 40 to 2022 (81,6%), both, from Bajo Grande and some neighborhoods in Cordoba city. HAV higher occurrence was in 2021 in the Pueyrredón neighborhood, particularly from April to July 2022, followed by Don Bosco and Marqués de Sobremonte. The number of HAV clinical cases notified was 1 in 2020, 3 in 2021, and 9 in 2022, mostly from March to May in adult patients (mean age 37 years old).
The HAV detection in wastewater confirms its circulation among the population of Córdoba, increasing its frequency simultaneously with the notification of clinical cases. Despite the low number of cases reported during 2020 and 2021, viral detection in wastewater during these years suggests a continuous silent circulation of HAV in the population, representing a potential risk for susceptible people. These results highlight the importance of wastewater-based epidemiology surveillance as a supplementary tool for clinical surveillance, to track the viral circulation trends dynamics in the population, and identify geographical hotspots of virus excretion. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39086 |
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I10-R327-article-390862024-04-15T16:14:45Z Monitoring of hepatitis A virus in wastewater as an environmental surveillance tool in Córdoba, Argentina Vigilancia ambiental como herramienta para el monitoreo del virus de hepatitis A en muestras de aguas residuales de Córdoba, Argentina . Fantilli , A Di Cola, G Castro , G Barbás , MG Nates , S Masachessi, G Pisano , MB Re, VE ENVIRONMENTAL SURVEILLANCE HAV wastewater vigilancia ambiental HAV aguas residuales . Due to hepatitis A virus (HAV) is a fecal-oral transmission pathogen, urban wastewater surveillance is considered a key tool to track the viral circulation trends over time in the population. The aim of this study was to assess HAV detection in wastewater samples from the city of Córdoba, Argentina, and to compare the results with the confirmed cases of hepatitis A illness reported during the same period. Sewage samples were weekly collected from May 2020 to July 2021, from “Bajo Grande”, one of the wastewater treatment plants located in Córdoba city. Furthermore, sewage collectors of 7 neighborhoods in Córdoba city were weekly sampled from January 2021 to July 2022. Virus concentration was carried out using PEG6000 and NaCl, and automated RNA extraction and HAV molecular detection by RT-qPCR were performed afterward. Finally, the dataset on the total number of confirmed HAV infected cases reported during the same period was requested to the Epidemiology area of the Government of the Province. Out of 748 samples analyzed, 49 were RNA-HAV+ (6.55%). One belonged to 2020 (2,04%), 8 to 2021 (16,3%), and 40 to 2022 (81,6%), both, from Bajo Grande and some neighborhoods in Cordoba city. HAV higher occurrence was in 2021 in the Pueyrredón neighborhood, particularly from April to July 2022, followed by Don Bosco and Marqués de Sobremonte. The number of HAV clinical cases notified was 1 in 2020, 3 in 2021, and 9 in 2022, mostly from March to May in adult patients (mean age 37 years old). The HAV detection in wastewater confirms its circulation among the population of Córdoba, increasing its frequency simultaneously with the notification of clinical cases. Despite the low number of cases reported during 2020 and 2021, viral detection in wastewater during these years suggests a continuous silent circulation of HAV in the population, representing a potential risk for susceptible people. These results highlight the importance of wastewater-based epidemiology surveillance as a supplementary tool for clinical surveillance, to track the viral circulation trends dynamics in the population, and identify geographical hotspots of virus excretion. Debido a que el virus de hepatitis A (HAV) es un agente patógeno de transmisión fecal-oral, se postula que las matrices acuosas residuales son útiles para monitorear la tendencia de la circulación poblacional de cepas virales. El objetivo fue evaluar la presencia de HAV en aguas residuales de la ciudad de Córdoba y comparar los resultados con la notificación de casos clínicos durante el mismo período. Se recolectaron semanalmente, muestras de aguas residuales de la planta “Bajo Grande” de la ciudad de Córdoba, desde mayo-2020 a julio-2022. Además, desde enero-2021 hasta julio-2022, se muestrearon semanalmente los colectores cloacales de 7 barrios de la ciudad. Se realizó concentración de virus utilizando PEG6000 y NaCl, extracción automatizada del RNA y detección molecular de HAV mediante PCR en tiempo real. Finalmente, se consultó la totalidad de casos notificados confirmados de hepatitis A durante el mismo período a Epidemiología del Gobierno de la Provincia. Se analizaron 748 muestras de aguas residuales; 49 fueron RNA-HAV positivas (6,55 %). Una pertenecía a 2020 (2,04%), 8 a 2021 (16,3%), y 40 a 2022 (81,6%), tanto de la planta de Bajo Grande, como de algunos barrios, siendo más frecuente en el barrio Pueyrredón, particularmente durante los meses de abril a julio 2022, seguido por Don Bosco y Marqués de Sobremonte. El número de notificaciones de hepatitis A fue 1 caso en 2020, 3 en 2021 y 9 en 2022, concentrados en los meses de marzo a mayo, en pacientes adultos (edad media de 37 años). La detección de HAV en aguas cloacales indica su circulación en la población de Córdoba, aumentando su frecuencia simultáneamente con la notificación de casos clínicos. A pesar del bajo número de casos reportados durante 2020 y 2021, la detección viral en aguas residuales durante estos años sugiere una continua circulación silenciosa de HAV en la población, representando un riesgo potencial para las personas susceptibles. Los resultados destacan la importancia de la vigilancia basada en aguas residuales como herramienta complementaria al monitoreo clínico, para identificar la tendencia de la dinámica de circulación viral, e identificar zonas geográficas críticas de excreción viral. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto . https://revistas.unc.edu.ar/index.php/med/article/view/39086 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |