IL6 genetic variants in the susceptibility to Trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy
In recent years, genetic approaches have been proposed to improve understanding of the causes of differential susceptibility to Trypanosoma cruzi infection and the wide clinical variability in Chagas disease progression. The vast majority of these studies have been carried out analyzing genes a...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39079 |
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I10-R327-article-39079 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
IL6 polymorphisms chagasic cardiomyopathy association study susceptibility to infection polimorfismos de IL6 miocardiopatia chagasica estudio de asociación susceptibilidad a la infección |
| spellingShingle |
IL6 polymorphisms chagasic cardiomyopathy association study susceptibility to infection polimorfismos de IL6 miocardiopatia chagasica estudio de asociación susceptibilidad a la infección Argüello Hoyos, P Velázquez López, DA Fernández Spector, H Strauss, M Bazán, C Blasco, R Rivarola, W Paglini, P Lo Presti, MS IL6 genetic variants in the susceptibility to Trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy |
| topic_facet |
IL6 polymorphisms chagasic cardiomyopathy association study susceptibility to infection polimorfismos de IL6 miocardiopatia chagasica estudio de asociación susceptibilidad a la infección |
| author |
Argüello Hoyos, P Velázquez López, DA Fernández Spector, H Strauss, M Bazán, C Blasco, R Rivarola, W Paglini, P Lo Presti, MS |
| author_facet |
Argüello Hoyos, P Velázquez López, DA Fernández Spector, H Strauss, M Bazán, C Blasco, R Rivarola, W Paglini, P Lo Presti, MS |
| author_sort |
Argüello Hoyos, P |
| title |
IL6 genetic variants in the susceptibility to Trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy |
| title_short |
IL6 genetic variants in the susceptibility to Trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy |
| title_full |
IL6 genetic variants in the susceptibility to Trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy |
| title_fullStr |
IL6 genetic variants in the susceptibility to Trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy |
| title_full_unstemmed |
IL6 genetic variants in the susceptibility to Trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy |
| title_sort |
il6 genetic variants in the susceptibility to trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy |
| description |
In recent years, genetic approaches have been proposed to improve understanding of the causes of differential susceptibility to Trypanosoma cruzi infection and the wide clinical variability in Chagas disease progression. The vast majority of these studies have been carried out analyzing genes associated with the immune response. The purpose of the present study was to analyze IL6 genetic variants (rs1800795) in the susceptibility to Trypanosoma cruzi infection and in the development of chronic chagasic cardiomyopathy (CCC), in patients from the Province of Córdoba (Argentina).
A total of 350 patients were recruited at the Hospital Nacional de Clínicas, the Sucre Clinic and the San Roque Hospital in the city of Córdoba. All participants underwent serological diagnosis for T. cruzi infection and were classified as seropositive (272) or seronegative (78). Based on the results of complementary tests and clinical findings, seropositive patients were classified as having CCC (182) and asymptomatic (90). The rs1800795 (G/C) polymorphism was determined by PCR-RFLP. Differences between allelic and genotypic frequencies were analyzed using chi-square/Fisher's exact test and logistic regression.
The frequency of the minor allele (C) was found to be increased in seronegative patients compared to seropositive patients, but no significant association was found between IL6 variants and susceptibility to T. cruzi infection after applying logistic regression adjusted for sex and age. The frequency of the C allele was higher in patients with CCC compared to asymptomatic individuals, but no significant association was found between IL6 variants and the development of cardiomyopathy after applying logistic regression adjusted for sex and age.
Our results suggest that IL6 genetic variants would not have a significant contribution to the susceptibility to infection and the development of chronic chagasic cardiomyopathy in the population studied, which is in agreement with previous studies carried out in other Latin American populations (Colombia and Peru).
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| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39079 |
| work_keys_str_mv |
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I10-R327-article-390792024-04-15T16:14:45Z IL6 genetic variants in the susceptibility to Trypanosoma cruzi infection and the development of chronic chagasic cardiomyopathy Variantes genéticas de IL6 y la susceptibilidad a la infección por Trypanosoma cruzi y el desarrollo de la miocardiopatía chagásica crónica Argüello Hoyos, P Velázquez López, DA Fernández Spector, H Strauss, M Bazán, C Blasco, R Rivarola, W Paglini, P Lo Presti, MS IL6 polymorphisms chagasic cardiomyopathy association study susceptibility to infection polimorfismos de IL6 miocardiopatia chagasica estudio de asociación susceptibilidad a la infección In recent years, genetic approaches have been proposed to improve understanding of the causes of differential susceptibility to Trypanosoma cruzi infection and the wide clinical variability in Chagas disease progression. The vast majority of these studies have been carried out analyzing genes associated with the immune response. The purpose of the present study was to analyze IL6 genetic variants (rs1800795) in the susceptibility to Trypanosoma cruzi infection and in the development of chronic chagasic cardiomyopathy (CCC), in patients from the Province of Córdoba (Argentina). A total of 350 patients were recruited at the Hospital Nacional de Clínicas, the Sucre Clinic and the San Roque Hospital in the city of Córdoba. All participants underwent serological diagnosis for T. cruzi infection and were classified as seropositive (272) or seronegative (78). Based on the results of complementary tests and clinical findings, seropositive patients were classified as having CCC (182) and asymptomatic (90). The rs1800795 (G/C) polymorphism was determined by PCR-RFLP. Differences between allelic and genotypic frequencies were analyzed using chi-square/Fisher's exact test and logistic regression. The frequency of the minor allele (C) was found to be increased in seronegative patients compared to seropositive patients, but no significant association was found between IL6 variants and susceptibility to T. cruzi infection after applying logistic regression adjusted for sex and age. The frequency of the C allele was higher in patients with CCC compared to asymptomatic individuals, but no significant association was found between IL6 variants and the development of cardiomyopathy after applying logistic regression adjusted for sex and age. Our results suggest that IL6 genetic variants would not have a significant contribution to the susceptibility to infection and the development of chronic chagasic cardiomyopathy in the population studied, which is in agreement with previous studies carried out in other Latin American populations (Colombia and Peru). En los últimos años se han propuesto enfoques genéticos para mejorar la comprensión de las causas de la susceptibilidad diferencial a la infección por Trypanosoma cruzi y la amplia variabilidad en la evolución de la enfermedad de Chagas. La gran mayoría de estos estudios se han realizado analizando genes asociados a la respuesta inmune. El objetivo del presente estudio fue analizar variantes genéticas de IL6 (rs1800795) en la susceptibilidad a la infección por Trypanosoma cruzi y en el desarrollo de la miocardiopatía chagásica crónica (CCC), en pacientes de la Provincia de Córdoba (Argentina). Se trabajó con un total de 350 pacientes, los cuales fueron reclutados en el Hospital Nacional de Clínicas, la Clínica Sucre y el Hospital San Roque de la ciudad de Córdoba. Todos los participantes se sometieron a un diagnóstico serológico para infección por T. cruzi y fueron clasificados como seropositivos (272) y seronegativos (78). Basado en los resultados de pruebas complementarias y hallazgos clínicos, los pacientes seropositivos se clasificaron como con CCC (182) y asintomáticos (90). El polimorfismo rs1800795 (G/C) se determinó por PCR-RFLP. Las diferencias entre las frecuencias alélicas y genotípicas se analizaron mediante chi cuadrado/test exacto de Fisher y regresión logística. La frecuencia del alelo menor C se encontró incrementada en los pacientes seronegativos en comparación con los seropositivos, pero no se encontró asociación significativa entre las variantes de IL6 y la susceptibilidad a la infección por T. cruzi después de aplicar regresión logística ajustada por sexo y edad. La frecuencia del alelo C fue mayor en pacientes con CCC en comparación con individuos asintomáticos, pero tampoco se encontró asociación significativa entre las variantes de IL6 y el desarrollo de la miocardiopatía después de aplicar regresión logística ajustada por sexo y edad. Nuestros resultados sugieren que las variantes genéticas de IL6 no tendrían un aporte significativo en la susceptibilidad a la infección y el desarrollo de miocardiopatía chagásica crónica en la población estudiada, lo que está en concordancia con estudios previos realizados en otras poblaciones latinoamericanas (Colombia y Perú). Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto https://revistas.unc.edu.ar/index.php/med/article/view/39079 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |