Evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies
Celiac disease is an immune-mediated disorder caused by gluten, characterized by the presence of clinical manifestations, specific antibodies, HLA-DQ2 or HLA-DQ8 haplotypes, and enteropathy. Specific antibodies include those directed against tissue transglutaminase: tTG (a-tTG); those directed again...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39058 |
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I10-R327-article-39058 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
Celiac disease IgA a-tTG IgA a-DGP EMA concordance ENFERMEDAD CELÍACA IgA a-DGP IgA a-tTG EMA concordancia . |
| spellingShingle |
Celiac disease IgA a-tTG IgA a-DGP EMA concordance ENFERMEDAD CELÍACA IgA a-DGP IgA a-tTG EMA concordancia . Martín, ML Pereira, B Frías, ME Zanotti, N Riga, C Petri , V Kohn, J Cassinerio, A Evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies |
| topic_facet |
Celiac disease IgA a-tTG IgA a-DGP EMA concordance ENFERMEDAD CELÍACA IgA a-DGP IgA a-tTG EMA concordancia . |
| author |
Martín, ML Pereira, B Frías, ME Zanotti, N Riga, C Petri , V Kohn, J Cassinerio, A |
| author_facet |
Martín, ML Pereira, B Frías, ME Zanotti, N Riga, C Petri , V Kohn, J Cassinerio, A |
| author_sort |
Martín, ML |
| title |
Evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies |
| title_short |
Evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies |
| title_full |
Evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies |
| title_fullStr |
Evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies |
| title_full_unstemmed |
Evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies |
| title_sort |
evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies |
| description |
Celiac disease is an immune-mediated disorder caused by gluten, characterized by the presence of clinical manifestations, specific antibodies, HLA-DQ2 or HLA-DQ8 haplotypes, and enteropathy. Specific antibodies include those directed against tissue transglutaminase: tTG (a-tTG); those directed against deaminated gliadin peptides (a-DGP) and anti-endomysium antibodies (EMA). The determination of specific antibodies must always be accompanied by the measurement of serum immunoglobulin A. If it is higher than 20 mg/dL, IgA isotype antibodies should be investigated, and if not, the IgG isotype. a-DGPs show lower performance compared to the other two antibodies. However, its use is suggested as an additional test, especially in children under 2 years of age when IgA a-tTG screening is negative. Objective: To evaluate the serological concordance between IgA a-DGP and EMA, considered the reference serological technique, in children under two years of age with negative IgA a-tTG.
Retrospective descriptive observational work. 123 patients under 2 years of age with serum IgA greater than 20 mg/dL and negative IgA a-tTG were given IgA-DGP and EMA. The statistical programs "InfoStat" free version and "MedCalc" version 10.2.0.0 were used. To assess the concordance of a-DGP with EMA, the Kappa index was used.
A concordance K index of 0.08 (95% CI -0.211; 0.364) was obtained between IgA a-DGP and EMA, the degree of agreement is insignificant.
The insignificant concordance between IgA a-DGP and EMA supports that the combination of tests (IgA a-tTG and IgA a-DGP) does not provide advantages in the diagnosis of celiac disease. The results of this work have a direct impact on the daily work of the immunology laboratory. The economic resources and the delivery time of the results will be optimized by modifying the diagnostic algorithm, with the performance of IgA a-tTG as the only screening test. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39058 |
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I10-R327-article-390582024-04-15T16:14:45Z Evaluation of antibodies against deaminated gliadin peptides in children under two years of age with negative anti-tissue transglutaminase antibodies Evaluación de anticuerpos anti péptidos de gliadina deaminada en niños menores de dos años con anticuerpos anti transglutaminasa tisular negativos. . Martín, ML Pereira, B Frías, ME Zanotti, N Riga, C Petri , V Kohn, J Cassinerio, A Celiac disease IgA a-tTG IgA a-DGP EMA concordance ENFERMEDAD CELÍACA IgA a-DGP IgA a-tTG EMA concordancia . Celiac disease is an immune-mediated disorder caused by gluten, characterized by the presence of clinical manifestations, specific antibodies, HLA-DQ2 or HLA-DQ8 haplotypes, and enteropathy. Specific antibodies include those directed against tissue transglutaminase: tTG (a-tTG); those directed against deaminated gliadin peptides (a-DGP) and anti-endomysium antibodies (EMA). The determination of specific antibodies must always be accompanied by the measurement of serum immunoglobulin A. If it is higher than 20 mg/dL, IgA isotype antibodies should be investigated, and if not, the IgG isotype. a-DGPs show lower performance compared to the other two antibodies. However, its use is suggested as an additional test, especially in children under 2 years of age when IgA a-tTG screening is negative. Objective: To evaluate the serological concordance between IgA a-DGP and EMA, considered the reference serological technique, in children under two years of age with negative IgA a-tTG. Retrospective descriptive observational work. 123 patients under 2 years of age with serum IgA greater than 20 mg/dL and negative IgA a-tTG were given IgA-DGP and EMA. The statistical programs "InfoStat" free version and "MedCalc" version 10.2.0.0 were used. To assess the concordance of a-DGP with EMA, the Kappa index was used. A concordance K index of 0.08 (95% CI -0.211; 0.364) was obtained between IgA a-DGP and EMA, the degree of agreement is insignificant. The insignificant concordance between IgA a-DGP and EMA supports that the combination of tests (IgA a-tTG and IgA a-DGP) does not provide advantages in the diagnosis of celiac disease. The results of this work have a direct impact on the daily work of the immunology laboratory. The economic resources and the delivery time of the results will be optimized by modifying the diagnostic algorithm, with the performance of IgA a-tTG as the only screening test. La enfermedad celíaca es un desorden inmuno-mediado provocado por el gluten, caracterizado por la presencia de manifestaciones clínicas, anticuerpos específicos, haplotipos HLA- DQ2 o HLA-DQ8 y enteropatía. Dentro de los anticuerpos específicos, se encuentran aquellos dirigidos contra la transglutaminasa tisular: tTG (a-tTG); los dirigidos contra los péptidos de gliadina deaminada (a-DGP) y los anticuerpos anti endomisio (EMA). La determinación de anticuerpos específicos siempre debe estar acompañada del dosaje de inmunoglobulina A sérica. Si la misma es superior a los 20 mg/dL se deben investigar los anticuerpos de isotipo IgA, y en caso contrario, el isotipo IgG. Los a-DGP presentan un rendimiento inferior en comparación con los otros dos anticuerpos. Sin embargo, su utilización es sugerida como una prueba adicional, especialmente en niños menores de 2 años cuando el screening con IgA a-tTG da negativo. Objetivo: Evaluar la concordancia serológica entre IgA a-DGP y EMA, considerada la técnica serológica de referencia, en niños menores de dos años con IgA a-tTG negativa. Trabajo observacional descriptivo retrospectivo. A 123 pacientes menores de 2 años con IgA sérica mayor a 20 mg/dL e IgA a-tTG negativa se les dosó IgA- DGP y EMA. Se utilizaron los programas estadísticos “InfoStat” versión libre y “MedCalc” versión 10.2.0.0. Para evaluar la concordancia de a-DGP con EMA, se utilizó el índice Kappa. Se obtuvo un índice de concordancia K de 0,08 (IC 95% -0,211; 0,364) entre IgA a-DGP y EMA, el grado de acuerdo es insignificante. La concordancia insignificante entre IgA a-DGP y EMA avala que la combinación de pruebas (IgA a-tTG e IgA a-DGP) no aporta ventajas en el diagnóstico de la enfermedad celíaca. Los resultados de este trabajo impactan de manera directa en el trabajo diario del laboratorio de inmunología. Los recursos económicos y el tiempo de entrega de los resultados se verán optimizados al modificar el algoritmo diagnóstico, con la realización de IgA a-tTG como única prueba de screening. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto . https://revistas.unc.edu.ar/index.php/med/article/view/39058 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |