Langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody CD-1a in uterine cervix
The integrity of the immune system is necessary to control tumor progression. Langerhans cells (LCs) plays a complex role in presentation and regulation of antigen processing. They are the first step to trigger and adjust an accurate adaptive immune response. We aim to analyze the presence...
| Autores principales: | , , |
|---|---|
| Formato: | Artículo revista |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
|
| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39053 |
| Aporte de: |
| id |
I10-R327-article-39053 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
langerhans cells squamous intraepithelial lesion squamous cell carcinoma uterine cervix celúlas de Langerhans lesión intraepitelial escamosa carcinoma epidermoide cuello uterino |
| spellingShingle |
langerhans cells squamous intraepithelial lesion squamous cell carcinoma uterine cervix celúlas de Langerhans lesión intraepitelial escamosa carcinoma epidermoide cuello uterino Gay Stabile, SL Juri, GA Dionisio de Cabalier , ME Langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody CD-1a in uterine cervix |
| topic_facet |
langerhans cells squamous intraepithelial lesion squamous cell carcinoma uterine cervix celúlas de Langerhans lesión intraepitelial escamosa carcinoma epidermoide cuello uterino |
| author |
Gay Stabile, SL Juri, GA Dionisio de Cabalier , ME |
| author_facet |
Gay Stabile, SL Juri, GA Dionisio de Cabalier , ME |
| author_sort |
Gay Stabile, SL |
| title |
Langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody CD-1a in uterine cervix |
| title_short |
Langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody CD-1a in uterine cervix |
| title_full |
Langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody CD-1a in uterine cervix |
| title_fullStr |
Langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody CD-1a in uterine cervix |
| title_full_unstemmed |
Langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody CD-1a in uterine cervix |
| title_sort |
langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody cd-1a in uterine cervix |
| description |
The integrity of the immune system is necessary to control tumor progression. Langerhans cells (LCs) plays a complex role in presentation and regulation of antigen processing. They are the first step to trigger and adjust an accurate adaptive immune response. We aim to analyze the presence and distribution pattern of the (LCs) in High-grade squamous intraepithelial lesions (High-grade SIL) and invasive squamous cell carcinoma (Invasive SCC) of the uterine cervix.
From a total of 920 non-binding uterine samples from the Pathology Service of the “Hospital Nacional de Clínicas”, a stratified random sampling was carried out, inclusion and exclusion criteria were applied resulting in 23 optimal samples; with this samples, two tissue microarrays were built, with 16 cores in total, including 2 cores of control tissue. Immunohistochemistry techniques were performed with the monoclonal antibody CD-1a. Images of these slides were captured with an optical microscope (Leica DM500), a digital camera (Leica ICC50 HD) and the "LAS EZ" software. The digitized images were processed with “ImageJ” software. “Rmedic” software was used for data processing. The results were expressed in summary measures and in statistical graphs. According to the distribution of the variables, t-test and Kruskal-Wallis test were performed. Statistical significance defined by a p value less than 0.05 was considered.
Statistically significant difference was found (p<0.001) between the number of LCs observed in the epithelium of High-grade SIL and Invasive SCC. The LCs's average had a distribution composed of suprabasal layer 14.15, intermediate layer 28.69 and superficial layer 5.69 in the High-grade SIL. While in the Invasive SCC the LCs presents a distribution composed of suprabasal layer 39.2, intermediate layer 102.8 and superficial layer 23.4.
The present study suggests that there is a recruitment of LCs in the malign neoplastic process. No changes were observed in the distribution pattern between the (High-grade SIL) and the (Invasive SCC). Apart from that, the intermediate and suprabasal layers exhibited the highest density of LCs. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39053 |
| work_keys_str_mv |
AT gaystabilesl langerhanscellsinsquamousintraepitheliallesionsandinvasivesquamouscellcarcinomausingantibodycd1ainuterinecervix AT juriga langerhanscellsinsquamousintraepitheliallesionsandinvasivesquamouscellcarcinomausingantibodycd1ainuterinecervix AT dionisiodecabalierme langerhanscellsinsquamousintraepitheliallesionsandinvasivesquamouscellcarcinomausingantibodycd1ainuterinecervix AT gaystabilesl celulasdelangerhansenlaslesionesintraepitelialescamosasyenelcarcinomaepidermoideinvasordecuellouterinoutilizandoinmunomarcacioncd1a AT juriga celulasdelangerhansenlaslesionesintraepitelialescamosasyenelcarcinomaepidermoideinvasordecuellouterinoutilizandoinmunomarcacioncd1a AT dionisiodecabalierme celulasdelangerhansenlaslesionesintraepitelialescamosasyenelcarcinomaepidermoideinvasordecuellouterinoutilizandoinmunomarcacioncd1a |
| first_indexed |
2024-09-03T21:04:04Z |
| last_indexed |
2024-09-03T21:04:04Z |
| _version_ |
1809210351093284864 |
| spelling |
I10-R327-article-390532024-04-15T16:14:45Z Langerhans cells in squamous intraepithelial lesions and invasive squamous cell carcinoma using antibody CD-1a in uterine cervix Células de Langerhans en las lesiones intraepitelial escamosas y en el carcinoma epidermoide invasor de cuello uterino, utilizando inmunomarcación CD-1a Gay Stabile, SL Juri, GA Dionisio de Cabalier , ME langerhans cells squamous intraepithelial lesion squamous cell carcinoma uterine cervix celúlas de Langerhans lesión intraepitelial escamosa carcinoma epidermoide cuello uterino The integrity of the immune system is necessary to control tumor progression. Langerhans cells (LCs) plays a complex role in presentation and regulation of antigen processing. They are the first step to trigger and adjust an accurate adaptive immune response. We aim to analyze the presence and distribution pattern of the (LCs) in High-grade squamous intraepithelial lesions (High-grade SIL) and invasive squamous cell carcinoma (Invasive SCC) of the uterine cervix. From a total of 920 non-binding uterine samples from the Pathology Service of the “Hospital Nacional de Clínicas”, a stratified random sampling was carried out, inclusion and exclusion criteria were applied resulting in 23 optimal samples; with this samples, two tissue microarrays were built, with 16 cores in total, including 2 cores of control tissue. Immunohistochemistry techniques were performed with the monoclonal antibody CD-1a. Images of these slides were captured with an optical microscope (Leica DM500), a digital camera (Leica ICC50 HD) and the "LAS EZ" software. The digitized images were processed with “ImageJ” software. “Rmedic” software was used for data processing. The results were expressed in summary measures and in statistical graphs. According to the distribution of the variables, t-test and Kruskal-Wallis test were performed. Statistical significance defined by a p value less than 0.05 was considered. Statistically significant difference was found (p<0.001) between the number of LCs observed in the epithelium of High-grade SIL and Invasive SCC. The LCs's average had a distribution composed of suprabasal layer 14.15, intermediate layer 28.69 and superficial layer 5.69 in the High-grade SIL. While in the Invasive SCC the LCs presents a distribution composed of suprabasal layer 39.2, intermediate layer 102.8 and superficial layer 23.4. The present study suggests that there is a recruitment of LCs in the malign neoplastic process. No changes were observed in the distribution pattern between the (High-grade SIL) and the (Invasive SCC). Apart from that, the intermediate and suprabasal layers exhibited the highest density of LCs. La integridad del sistema inmunológico es necesaria para controlar la progresión tumoral. Las células de Langerhans (CL) presentan una función compleja en la regulación del procesamiento de antígenos y la presentación del mismo, siendo el primer paso para desencadenar una respuesta inmunitaria adaptativa adecuada. El objetivo del presente trabajo fue analizar la presencia y el patrón de distribución de las CL en la Lesión Intraepitelial escamosa de Alto grado (LIE de alto grado) y en el carcinoma epidermoide invasor (CEI) del cuello uterino. De un total de 920 piezas uterinas, no vinculantes del archivo del Servicio de Patología del Hospital Nacional de Clínicas, se realizó un muestreo aleatorio estratificado, se aplicó criterios de inclusión y exclusión y se finalizó con 23 muestras óptimas, con las que se construyeron dos tissue microarray, con 16 core en total, que incluían 2 core de tejido control. Se efectuaron técnicas de inmunomarcación con el anticuerpo monoclonal CD-1a. Se realizó la captura de imágenes de dichos slides con un microscopio óptico (Leica DM500), una cámara digital (Leica ICC50 HD) y el software “LAS EZ”. Las imágenes digitalizadas se procesaron con el software “ImageJ”. Para el tratamiento de los datos se utilizó el software Rmedic. Los resultados se expresaron en medidas de resumen y en gráfico estadísticos. Según la distribución de las variables se realizó test t y Test de Kruskal-Wallis. Se consideró la significancia estadística definida por un valor p menor que 0,05. Los resultados más relevantes encontrados fueron: una diferencia estadísticamente significativa p<0,001 entre en número de CL presente en el epitelio de las LIE de alto grado y el CEI; la media de distribución en el LIE de alto grado son: Suprabasal layer 14,15; Intermedia layer 28,69; Superficial layer 5,69; mientas que el CEI presenta: Suprabasal 39,2; Intermedia 102,8; Superficial 23,4. El presente estudio sugiere que existe un reclutamiento de CL en el proceso neoplásico maligno. El patrón de distribución se mantiene constante tanto en el CEI como en el LIE de alto grado, siendo las capas intermedias y suprabasales las que presenta mayor número de CL. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion . https://revistas.unc.edu.ar/index.php/med/article/view/39053 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |