Impact of metabolic syndrome on testicular redox status: role of an antioxidant
Metabolic syndrome (MS) has a harmful effect on the male reproductive system. This work was aimed to elucidate the mechanisms by which MS is affecting tissues of the male reproductive system and the possible role of quercetin (QT) to revert these alterations. Male Wistar rats were divided i...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39048 |
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I10-R327-article-39048 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
metabolic syndrome testis oxidative stress quercetin síndrome metabólico testículo estrés oxidativo quercetina |
| spellingShingle |
metabolic syndrome testis oxidative stress quercetin síndrome metabólico testículo estrés oxidativo quercetina Cuevas Ibarra, AM Luciani, N Torres, P Luque, E Martini, A Rivoira, MA Diaz de Barboza , G Silvano, L Impact of metabolic syndrome on testicular redox status: role of an antioxidant |
| topic_facet |
metabolic syndrome testis oxidative stress quercetin síndrome metabólico testículo estrés oxidativo quercetina |
| author |
Cuevas Ibarra, AM Luciani, N Torres, P Luque, E Martini, A Rivoira, MA Diaz de Barboza , G Silvano, L |
| author_facet |
Cuevas Ibarra, AM Luciani, N Torres, P Luque, E Martini, A Rivoira, MA Diaz de Barboza , G Silvano, L |
| author_sort |
Cuevas Ibarra, AM |
| title |
Impact of metabolic syndrome on testicular redox status: role of an antioxidant |
| title_short |
Impact of metabolic syndrome on testicular redox status: role of an antioxidant |
| title_full |
Impact of metabolic syndrome on testicular redox status: role of an antioxidant |
| title_fullStr |
Impact of metabolic syndrome on testicular redox status: role of an antioxidant |
| title_full_unstemmed |
Impact of metabolic syndrome on testicular redox status: role of an antioxidant |
| title_sort |
impact of metabolic syndrome on testicular redox status: role of an antioxidant |
| description |
Metabolic syndrome (MS) has a harmful effect on the male reproductive system. This work was aimed to elucidate the mechanisms by which MS is affecting tissues of the male reproductive system and the possible role of quercetin (QT) to revert these alterations.
Male Wistar rats were divided into: 1) controls; 2) MS (treated with 10% fructose in the drinking water for 60 days); 3) MS+ QT (50 mg/kg b.w. during last 15 days of fructose treatment); 4) control+ QT (50 mg/kg b.w. during last 15 days). Waist circumference and body weight were measured in control and treated rats. Glucose, triglycerides, cholesterol, HDLc and testosterone were determined in blood samples. Sperm concentration, motility and acrosomal reaction were evaluated from the epididymis. Spermatogenesis and morphology were assessed in testicular sections stained with hematoxylin-PAS. Glutathione (GSH) content and superoxide dismutase (SOD) activity were determined in testis homogenates. Results were evaluated by one-way analysis of variance (ANOVA) and Tukey’s test as a post hoc.
Waist circumference, body weight, and serum triglycerides were increased and HDLc was decreased in MS rats compared to control values. All animals showed complete spermatogenesis. In MS rats, testosterone level (C:4.72±0.60; MS:2.43±0.31*; MS+QT:4.39±0.52 ng/dL; *p<0.05 vs C and MS+QT) and the motile spermatozoa percentage (C:61.7±2.33; MS:47.3±2.01*; MS+QT:74.6±2.12 %, *p<0.05 vs C and MS+QT) were lower than controls whereas QT restored those parameters. The seminiferous tubules area from MS rats was smaller than Control group. The MS rats presented lower GSH content (C:54.27±3.16; MS:39.22±1.90*; MS+QT:58.30±2.64 nmol/mg prot., *p<0.05 vs C and MS+QT) and higher SOD activity (C:9.24±0.69; MS:15.60±1.50*; MS+QT:10.95±1.92 UI/mg prot, *p<0.05 vs C and MS+QT) compared to those from the control rats. QT administration prevented those alterations and additionally, decreased spontaneous acrosomal reaction.
The results suggest that MS induced by fructose intake could decrease the testosterone synthesis and the sperm motility as a consequence of oxidative stress. QT treatment could improve the testis oxidative state and the general conditions of MS. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39048 |
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I10-R327-article-390482024-04-15T16:14:45Z Impact of metabolic syndrome on testicular redox status: role of an antioxidant Impacto del síndrome metabólico experimental sobre el estado redox testicular: rol de un antioxidante Cuevas Ibarra, AM Luciani, N Torres, P Luque, E Martini, A Rivoira, MA Diaz de Barboza , G Silvano, L metabolic syndrome testis oxidative stress quercetin síndrome metabólico testículo estrés oxidativo quercetina Metabolic syndrome (MS) has a harmful effect on the male reproductive system. This work was aimed to elucidate the mechanisms by which MS is affecting tissues of the male reproductive system and the possible role of quercetin (QT) to revert these alterations. Male Wistar rats were divided into: 1) controls; 2) MS (treated with 10% fructose in the drinking water for 60 days); 3) MS+ QT (50 mg/kg b.w. during last 15 days of fructose treatment); 4) control+ QT (50 mg/kg b.w. during last 15 days). Waist circumference and body weight were measured in control and treated rats. Glucose, triglycerides, cholesterol, HDLc and testosterone were determined in blood samples. Sperm concentration, motility and acrosomal reaction were evaluated from the epididymis. Spermatogenesis and morphology were assessed in testicular sections stained with hematoxylin-PAS. Glutathione (GSH) content and superoxide dismutase (SOD) activity were determined in testis homogenates. Results were evaluated by one-way analysis of variance (ANOVA) and Tukey’s test as a post hoc. Waist circumference, body weight, and serum triglycerides were increased and HDLc was decreased in MS rats compared to control values. All animals showed complete spermatogenesis. In MS rats, testosterone level (C:4.72±0.60; MS:2.43±0.31*; MS+QT:4.39±0.52 ng/dL; *p<0.05 vs C and MS+QT) and the motile spermatozoa percentage (C:61.7±2.33; MS:47.3±2.01*; MS+QT:74.6±2.12 %, *p<0.05 vs C and MS+QT) were lower than controls whereas QT restored those parameters. The seminiferous tubules area from MS rats was smaller than Control group. The MS rats presented lower GSH content (C:54.27±3.16; MS:39.22±1.90*; MS+QT:58.30±2.64 nmol/mg prot., *p<0.05 vs C and MS+QT) and higher SOD activity (C:9.24±0.69; MS:15.60±1.50*; MS+QT:10.95±1.92 UI/mg prot, *p<0.05 vs C and MS+QT) compared to those from the control rats. QT administration prevented those alterations and additionally, decreased spontaneous acrosomal reaction. The results suggest that MS induced by fructose intake could decrease the testosterone synthesis and the sperm motility as a consequence of oxidative stress. QT treatment could improve the testis oxidative state and the general conditions of MS. El Síndrome Metabólico (SM) puede producir daño a nivel del sistema reproductor masculino. El objetivo de este trabajo fue dilucidar los mecanismos por los cuales el SM afecta a los tejidos del sistema reproductor masculino y determinar si estas alteraciones pueden ser revertidas por la administración de quercetina (QT). Ratas Wistar machos adultas se dividieron: 1) controles; 2) SM, tratadas con fructosa al 10% (P/V) en el agua de bebida por 60 días; 3) SM+QT por vía oral (50 mg/kg peso corporal, pc) los últimos 15 días del tratamiento; 4) control+QT por vía oral (50 mg/kg pc) los últimos 15 días. Se midió perímetro de cintura, peso corporal y, además, en suero se determinó glucosa, triglicéridos, colesterol, HDLc y testosterona. En epidídimo se evaluó concentración, motilidad y reacción acrosomal y en secciones testiculares (hematoxilina-PAS) se analizó la espermatogénesis y la morfología. En homogeneizado de testículo se cuantificó el contenido de glutatión (GSH) y la actividad de superóxido dismutasa (SOD). Para análisis estadístico se realizó ANOVA y Tukey. Las ratas SM presentaron aumento del perímetro de cintura, peso corporal, trigliceridemia y disminución de HDLc en comparación con los valores controles. Todos los animales presentaron una espermatogénesis completa. En el SM la testosterona (C:4,72±0,60; SM:2,43±0,31*; SM+QT:4,39±0,52 ng/dL; *p<0,05 vs C y SM+QT) y el porcentaje de espermatozoides móviles (C:61,7±2,33; SM:47,3±2,01*; SM+QT:74,6±2,12 %, *p<0,05 vs C y SM+QT) fueron menores que el de los controles, la administración de QT restauró esos parámetros. El área de los túbulos seminíferos fue menor en el SM. En este grupo el contenido de GSH disminuyó (C:54,27±3,16; SM:39,22±1,90*; SM+QT:58,30±2,64 nmol/mg prot., *p<0,05 vs C y SM+QT) y la actividad de SOD aumentó (C:9,24±0,69; SM:15,60±1,50*; SM+QT:10,95±1,92 UI/mg prot. *p<0,05 vs C y SM+QT), el antioxidante lo previno. QT disminuyó la reacción acrosomal espontánea. Los resultados sugieren que el SM producido por la ingesta de fructosa produce estrés oxidativo y disminuye la síntesis de testosterona. Estos podrían ser, al menos en parte, los responsables de la disminución de la motilidad espermática. La administración de QT revierte el estado oxidado del testículo y mejora las condiciones generales del SM. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/39048 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |