Metabolic syndrome in dams and fetal programming: a study in a murine model
Metabolic syndrome (MS) is a highly prevalent in our country, even in women at gestational age. However, its potential impact as a fetal programmer was rarely evaluated. The hypothesis of our study was that dams MS alters the development of the offspring, impacting on litters...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39039 |
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I10-R327-article-39039 |
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ojs |
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Universidad Nacional de Córdoba |
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I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
metabolic syndrome fetal programming reproduction postnatal development sexual maturation síndrome metabólico programación fetal reproduccion desarrollo posnatal maduración sexual |
| spellingShingle |
metabolic syndrome fetal programming reproduction postnatal development sexual maturation síndrome metabólico programación fetal reproduccion desarrollo posnatal maduración sexual Luque, EM Gerbaldo , V Torres, PJ Ramírez, N Arja, A Martini, AC Metabolic syndrome in dams and fetal programming: a study in a murine model |
| topic_facet |
metabolic syndrome fetal programming reproduction postnatal development sexual maturation síndrome metabólico programación fetal reproduccion desarrollo posnatal maduración sexual |
| author |
Luque, EM Gerbaldo , V Torres, PJ Ramírez, N Arja, A Martini, AC |
| author_facet |
Luque, EM Gerbaldo , V Torres, PJ Ramírez, N Arja, A Martini, AC |
| author_sort |
Luque, EM |
| title |
Metabolic syndrome in dams and fetal programming: a study in a murine model |
| title_short |
Metabolic syndrome in dams and fetal programming: a study in a murine model |
| title_full |
Metabolic syndrome in dams and fetal programming: a study in a murine model |
| title_fullStr |
Metabolic syndrome in dams and fetal programming: a study in a murine model |
| title_full_unstemmed |
Metabolic syndrome in dams and fetal programming: a study in a murine model |
| title_sort |
metabolic syndrome in dams and fetal programming: a study in a murine model |
| description |
Metabolic syndrome (MS) is a highly prevalent in our country, even in women at gestational age. However, its potential impact as a fetal programmer was rarely evaluated. The hypothesis of our study was that dams MS alters the development of the offspring, impacting on litters growth and postnatal development. Hence, we studied in a model of pregnant rats (F0) with MS, the effects of this pathology on: 1) pregnancy rate, duration of gestation and litter size and weight; 2) physical, neurobiological and sexual development of the offspring (F1) and 3) reproductive function of the F1 in adulthood.
We used adult female Wistar rats, randomly divided into two groups: a) controls (C): pelleted food + water; and b) SM: pelleted food + water with 10% fructose; n=8 animals/treatment. The treatment was applied from 4 weeks previous to copula, until pups weaning. Results were analyzed with ANOVA, repeated measures ANOVA or Chi-square, as appropriate, and p≤0.05 as significance level.
In F0, MS was verified with a significant increase in total cholesterol (MS=87.9±1.6mg/dl vs C=65.0±6.4mg/dl), triglycerides (MS=136.2±18.3mg /dl vs C=79.5±11.9mg/dl), LDL (SM=24.3±4.5mg/dl vs C=9.8±3.5mg/dl) and TG/HDL (SM=4 .1±0.7 vs C= 2.0±0.1). Furthermore, these females gained significantly more weight (SM=35.5±3.4g vs C=23.9±3.7g) and had more visceral fat (SM=11.7±2.3g vs C=6.4 ±1.3g). In F1, SM increased significantly body weight gain of male pups (SM=59.52±0.66g vs C=49.67±2.24g) and advanced vaginal opening (day 28: SM=46, 7±29.0% vs C=10.0±5.0%) and testicular descent (day 19: SM=100.0±0.0% vs C=11.67±5.43%); n=6-4 litters/treatment. In adulthood, F1 female pups from the SM group (n=12) showed, at gestation day 18, significantly increased body weight (SM=280.4±3.9g vs C=251.5±7.0g without pups), higher amounts of corpora lutea (SM=15.00±0.4 vs C=12.25±0.5), heavier fetuses (SM=2.2±0.2g vs C=1.7±0.2g) and higher frequency of embryonic loss (SM=13.3±4.5% vs C=3.6±1.2%) than control pups (n=10).
Under our experimental conditions, it can be confirmed that MS acts as a fetal programmer, modeling the physical and sexual development of the offspring and the reproductive function of the female pups in adulthood.
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| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39039 |
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I10-R327-article-390392024-04-15T16:14:45Z Metabolic syndrome in dams and fetal programming: a study in a murine model Síndrome metabólico materno y programación fetal: estudio en un modelo murino Luque, EM Gerbaldo , V Torres, PJ Ramírez, N Arja, A Martini, AC metabolic syndrome fetal programming reproduction postnatal development sexual maturation síndrome metabólico programación fetal reproduccion desarrollo posnatal maduración sexual Metabolic syndrome (MS) is a highly prevalent in our country, even in women at gestational age. However, its potential impact as a fetal programmer was rarely evaluated. The hypothesis of our study was that dams MS alters the development of the offspring, impacting on litters growth and postnatal development. Hence, we studied in a model of pregnant rats (F0) with MS, the effects of this pathology on: 1) pregnancy rate, duration of gestation and litter size and weight; 2) physical, neurobiological and sexual development of the offspring (F1) and 3) reproductive function of the F1 in adulthood. We used adult female Wistar rats, randomly divided into two groups: a) controls (C): pelleted food + water; and b) SM: pelleted food + water with 10% fructose; n=8 animals/treatment. The treatment was applied from 4 weeks previous to copula, until pups weaning. Results were analyzed with ANOVA, repeated measures ANOVA or Chi-square, as appropriate, and p≤0.05 as significance level. In F0, MS was verified with a significant increase in total cholesterol (MS=87.9±1.6mg/dl vs C=65.0±6.4mg/dl), triglycerides (MS=136.2±18.3mg /dl vs C=79.5±11.9mg/dl), LDL (SM=24.3±4.5mg/dl vs C=9.8±3.5mg/dl) and TG/HDL (SM=4 .1±0.7 vs C= 2.0±0.1). Furthermore, these females gained significantly more weight (SM=35.5±3.4g vs C=23.9±3.7g) and had more visceral fat (SM=11.7±2.3g vs C=6.4 ±1.3g). In F1, SM increased significantly body weight gain of male pups (SM=59.52±0.66g vs C=49.67±2.24g) and advanced vaginal opening (day 28: SM=46, 7±29.0% vs C=10.0±5.0%) and testicular descent (day 19: SM=100.0±0.0% vs C=11.67±5.43%); n=6-4 litters/treatment. In adulthood, F1 female pups from the SM group (n=12) showed, at gestation day 18, significantly increased body weight (SM=280.4±3.9g vs C=251.5±7.0g without pups), higher amounts of corpora lutea (SM=15.00±0.4 vs C=12.25±0.5), heavier fetuses (SM=2.2±0.2g vs C=1.7±0.2g) and higher frequency of embryonic loss (SM=13.3±4.5% vs C=3.6±1.2%) than control pups (n=10). Under our experimental conditions, it can be confirmed that MS acts as a fetal programmer, modeling the physical and sexual development of the offspring and the reproductive function of the female pups in adulthood. El síndrome metabólico (SM) es una patología de alta prevalencia en nuestro país, incluso en mujeres en edad gestacional. Sin embargo, poco es lo que se conoce acerca de su posible impacto como programador fetal. Nuestra hipótesis es que el SM materno afecta el desarrollo de la descendencia, pudiendo provocar modificaciones en el crecimiento y la maduración posnatal de la camada. Estudiamos en un modelo de ratas preñadas (F0) con SM, los efectos de esta patología sobre: 1) tasa de preñez, duración de la gestación, tamaño y peso de la camada, 2) desarrollo físico, neurobiológico y sexual de las crías (F1) y 3) capacidad reproductiva de éstas en la adultez. Utilizamos ratas Wistar hembras adultas, divididas en dos grupos: a) controles (C): alimento balanceado+agua y b) SM: alimento balanceado+agua con 10% de fructosa; n=8 animales/tratamiento. El tratamiento se aplicó desde 4 semanas previas a la cópula, hasta el destete de las crías. Los resultados se analizaron con ANOVA (común o de medidas repetidas) o Chi-cuadrado según corresponda. Con un nivel de significancia de p≤0,05. En la F0, se verificó el SM con aumento de colesterol total (SM=87,9±1,6mg/dl vs C=65,0±6,4mg/dl), triglicéridos (SM=136,2±18,3mg/dl vs C=79,5±11,9mg/dl), LDL (SM=24,3±4,5mg/dl vs C=9,8±3,5mg/dl) y TG/HDL (SM=4,1±0,7 vs C= 2,0±0,1). Además, esas hembras ganaron significativamente más peso (SM=35,5±3,4g vs C=23,9±3,7g) y presentaron más grasa visceral (SM=11,7±2,3g vs C=6,4±1,3g). En la F1, el SM aumentó la ganancia de peso corporal de las crías macho (SM=59,52±0,66g vs C=49,67±2,24g) y adelantó la apertura vaginal (día 28: SM=46,7±29,0% vs C=10,0±5,0%) y el descenso testicular (día 19: SM=100,0±0,0% vs C=11,67±5,43%); n=6-4 camadas/tratamiento. En la adultez (F1), las crías hembras SM (n=12), al día 18 de gestación, presentaron mayor peso corporal (SM=280,4±3,9g vs C=251,5±7,0g sin crías), mayor cantidad de cuerpos lúteos (SM=15,00±0,4 vs C=12,25±0,5), fetos de mayor peso (SM=2,2±0,2g vs C=1,7±0,2g) y mayor frecuencia de pérdida embrionaria (SM=13,3±4,5% vs C=3,6±1,2%) que las crías controles (n=10). Bajo nuestras condiciones experimentales se puede afirmar que el SM actúa como programador fetal, modelando el desarrollo físico y sexual de las crías y la función reproductiva de las crías hembras adultas. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto https://revistas.unc.edu.ar/index.php/med/article/view/39039 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |