Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis
Uveitis is an internal inflammation of the eye that affects the uvea, with anti-inflammatory drugs being the main treatment and the most effective being injectable triamcinolone acetonide (TA). There is currently no formulation for topical use of TA. Objective: Evaluate the efficacy and toxicity of...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39035 |
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| Sumario: | Uveitis is an internal inflammation of the eye that affects the uvea, with anti-inflammatory drugs being the main treatment and the most effective being injectable triamcinolone acetonide (TA). There is currently no formulation for topical use of TA. Objective: Evaluate the efficacy and toxicity of topical TA-loaded nanometric lipid nanocapsules (TA-NCL) in a rabbit model of uveitis.
14 New Zealand type rabbits were used for the development of the uveitis model by intravitreal inoculation of 60 mg of a lipopolysaccharide. They were divided into two groups: Control group (n = 5) treated with physiological solution, and study group (n = 9) treated with TA-NCL, with topical applications twice a day. The evaluation of both groups was carried out through clinical analysis of the ocular surface and the internal structures of the eye, with ophthalmological equipment. Clinical variables of inflammation were observed and score tables were configured, taking into account flare, fibrin, and alterations in the conjunctiva. Values from zero to four were considered for each variable. Flare and fibrin were multiplied by three and two, respectively, in correlation with their implications for the degree of inflammation. For the total score, the values of the three variables were added and the results were grouped into: severe (18-24), severe (12-17), moderate (8-11) and mild (<8). The data was analyzed with the Student's t-test for independent samples, establishing a significance level of p≤0.05. Protocols according to guidelines established by the CICUAL (FCM-UNC) (No. 44/2017).
At 24 hours after injection, severe inflammation (14.5) was observed in the control group, decreasing to moderate on day 4 (9.7), while in the group under treatment with TA-NCL, at 24 hours, mild inflammation was determined (6.3), decreasing to almost normal parameters on the last day (1.5) (p=0.0018).
Topical application of TA-NCL was effective in rabbits with induced uveitis, evidenced by a significant decrease in intraocular and superficial inflammatory parameters compared to the control group, without causing adverse reactions. |
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