Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis
Uveitis is an internal inflammation of the eye that affects the uvea, with anti-inflammatory drugs being the main treatment and the most effective being injectable triamcinolone acetonide (TA). There is currently no formulation for topical use of TA. Objective: Evaluate the efficacy and toxicity of...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39035 |
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I10-R327-article-39035 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
lipid nanocapsules triamcinolone uveítis treatment nanocápsulas lipídicas triamcinolona uveítis tratamiento |
| spellingShingle |
lipid nanocapsules triamcinolone uveítis treatment nanocápsulas lipídicas triamcinolona uveítis tratamiento Bacigalupo, L Angós, J Awde Alfonso, HG Campana, V Palma, S Formica, L Tártara, LI Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis |
| topic_facet |
lipid nanocapsules triamcinolone uveítis treatment nanocápsulas lipídicas triamcinolona uveítis tratamiento |
| author |
Bacigalupo, L Angós, J Awde Alfonso, HG Campana, V Palma, S Formica, L Tártara, LI |
| author_facet |
Bacigalupo, L Angós, J Awde Alfonso, HG Campana, V Palma, S Formica, L Tártara, LI |
| author_sort |
Bacigalupo, L |
| title |
Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis |
| title_short |
Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis |
| title_full |
Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis |
| title_fullStr |
Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis |
| title_full_unstemmed |
Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis |
| title_sort |
evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis |
| description |
Uveitis is an internal inflammation of the eye that affects the uvea, with anti-inflammatory drugs being the main treatment and the most effective being injectable triamcinolone acetonide (TA). There is currently no formulation for topical use of TA. Objective: Evaluate the efficacy and toxicity of topical TA-loaded nanometric lipid nanocapsules (TA-NCL) in a rabbit model of uveitis.
14 New Zealand type rabbits were used for the development of the uveitis model by intravitreal inoculation of 60 mg of a lipopolysaccharide. They were divided into two groups: Control group (n = 5) treated with physiological solution, and study group (n = 9) treated with TA-NCL, with topical applications twice a day. The evaluation of both groups was carried out through clinical analysis of the ocular surface and the internal structures of the eye, with ophthalmological equipment. Clinical variables of inflammation were observed and score tables were configured, taking into account flare, fibrin, and alterations in the conjunctiva. Values from zero to four were considered for each variable. Flare and fibrin were multiplied by three and two, respectively, in correlation with their implications for the degree of inflammation. For the total score, the values of the three variables were added and the results were grouped into: severe (18-24), severe (12-17), moderate (8-11) and mild (<8). The data was analyzed with the Student's t-test for independent samples, establishing a significance level of p≤0.05. Protocols according to guidelines established by the CICUAL (FCM-UNC) (No. 44/2017).
At 24 hours after injection, severe inflammation (14.5) was observed in the control group, decreasing to moderate on day 4 (9.7), while in the group under treatment with TA-NCL, at 24 hours, mild inflammation was determined (6.3), decreasing to almost normal parameters on the last day (1.5) (p=0.0018).
Topical application of TA-NCL was effective in rabbits with induced uveitis, evidenced by a significant decrease in intraocular and superficial inflammatory parameters compared to the control group, without causing adverse reactions. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39035 |
| work_keys_str_mv |
AT bacigalupol evaluationofthetopicaleffectivenessoflipidnanocapsulesoftriamcinoloneforthetreatmentofuveitis AT angosj evaluationofthetopicaleffectivenessoflipidnanocapsulesoftriamcinoloneforthetreatmentofuveitis AT awdealfonsohg evaluationofthetopicaleffectivenessoflipidnanocapsulesoftriamcinoloneforthetreatmentofuveitis AT campanav evaluationofthetopicaleffectivenessoflipidnanocapsulesoftriamcinoloneforthetreatmentofuveitis AT palmas evaluationofthetopicaleffectivenessoflipidnanocapsulesoftriamcinoloneforthetreatmentofuveitis AT formical evaluationofthetopicaleffectivenessoflipidnanocapsulesoftriamcinoloneforthetreatmentofuveitis AT tartarali evaluationofthetopicaleffectivenessoflipidnanocapsulesoftriamcinoloneforthetreatmentofuveitis AT bacigalupol evaluaciondelaefectividadtopicadenanocapsulaslipidicasdetriamcinolonaparaeltratamientodelauveitis AT angosj evaluaciondelaefectividadtopicadenanocapsulaslipidicasdetriamcinolonaparaeltratamientodelauveitis AT awdealfonsohg evaluaciondelaefectividadtopicadenanocapsulaslipidicasdetriamcinolonaparaeltratamientodelauveitis AT campanav evaluaciondelaefectividadtopicadenanocapsulaslipidicasdetriamcinolonaparaeltratamientodelauveitis AT palmas evaluaciondelaefectividadtopicadenanocapsulaslipidicasdetriamcinolonaparaeltratamientodelauveitis AT formical evaluaciondelaefectividadtopicadenanocapsulaslipidicasdetriamcinolonaparaeltratamientodelauveitis AT tartarali evaluaciondelaefectividadtopicadenanocapsulaslipidicasdetriamcinolonaparaeltratamientodelauveitis |
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2024-09-03T21:04:01Z |
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2024-09-03T21:04:01Z |
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I10-R327-article-390352024-04-15T16:14:45Z Evaluation of the topical effectiveness of lipid nanocapsules of triamcinolone for the treatment of uveitis Evaluación de la efectividad tópica de nanocápsulas lipídicas de triamcinolona para el tratamiento de la uveítis Bacigalupo, L Angós, J Awde Alfonso, HG Campana, V Palma, S Formica, L Tártara, LI lipid nanocapsules triamcinolone uveítis treatment nanocápsulas lipídicas triamcinolona uveítis tratamiento Uveitis is an internal inflammation of the eye that affects the uvea, with anti-inflammatory drugs being the main treatment and the most effective being injectable triamcinolone acetonide (TA). There is currently no formulation for topical use of TA. Objective: Evaluate the efficacy and toxicity of topical TA-loaded nanometric lipid nanocapsules (TA-NCL) in a rabbit model of uveitis. 14 New Zealand type rabbits were used for the development of the uveitis model by intravitreal inoculation of 60 mg of a lipopolysaccharide. They were divided into two groups: Control group (n = 5) treated with physiological solution, and study group (n = 9) treated with TA-NCL, with topical applications twice a day. The evaluation of both groups was carried out through clinical analysis of the ocular surface and the internal structures of the eye, with ophthalmological equipment. Clinical variables of inflammation were observed and score tables were configured, taking into account flare, fibrin, and alterations in the conjunctiva. Values from zero to four were considered for each variable. Flare and fibrin were multiplied by three and two, respectively, in correlation with their implications for the degree of inflammation. For the total score, the values of the three variables were added and the results were grouped into: severe (18-24), severe (12-17), moderate (8-11) and mild (<8). The data was analyzed with the Student's t-test for independent samples, establishing a significance level of p≤0.05. Protocols according to guidelines established by the CICUAL (FCM-UNC) (No. 44/2017). At 24 hours after injection, severe inflammation (14.5) was observed in the control group, decreasing to moderate on day 4 (9.7), while in the group under treatment with TA-NCL, at 24 hours, mild inflammation was determined (6.3), decreasing to almost normal parameters on the last day (1.5) (p=0.0018). Topical application of TA-NCL was effective in rabbits with induced uveitis, evidenced by a significant decrease in intraocular and superficial inflammatory parameters compared to the control group, without causing adverse reactions. La uveítis es una inflamación interna del ojo que afecta a la úvea, siendo los antiinflamatorios el principal tratamiento y el más efectivo el acetónido de triamcinolona (TA) inyectable. No existe actualmente una formulación para uso tópico de TA. Objetivo: Evaluar la eficacia y toxicidad de nanocápsulas lipídicas nanométricas cargadas con TA (TA-NCL) tópica en un modelo de uveítis en conejos. Se utilizaron 14 conejos tipo New Zealand para el desarrollo del modelo de uveítis mediante inoculación intravítrea de 60 mg de un lipopolisacárido. Se dividieron en dos grupos: Grupo control (n =5) tratado con solución fisiológica, y grupo en estudio (n=9) tratado con TA-NCL, con aplicaciones tópicas dos veces al día. La evaluación de ambos grupos se realizó mediante el análisis clínico de la superficie ocular y las estructuras internas del ojo, con aparatología oftalmológica. Se observaron variables clínicas de inflamación y se configuraron tablas de score, teniendo en cuenta flare, fibrina, y alteraciones en la conjuntiva. Se consideraron valores de cero a cuatro en cada variable. Flare y fibrina fueron multiplicadas por tres y dos respectivamente, en correlación con sus implicancias en el grado de inflamación. Para el score total se sumaron los valores de las tres variables y se agruparon los resultados en: severo (18-24), grave (12-17), moderado (8-11) y leve (<8). Los datos fueron analizados con la prueba T de Student para muestras independientes, estableciendo un nivel de significación de p≤0,05. Protocolos acordes a pautas establecidas por el CICUAL (FCM-UNC) (Nº 44/2017). A las 24hs de la inyección en el grupo control se observó inflamación severa (14.5) descendiendo a moderado el día 4 (9.7), mientras que en el grupo bajo tratamiento con TA-NCL a las 24hs se determinó una inflamación leve (6.3) descendiendo a parámetros casi normales al último día (1.5) (p=0.0018). La aplicación tópica de TA-NCL fue efectiva en conejos con uveítis inducida, evidenciado por un descenso significativo de parámetros inflamatorios intraoculares y superficiales comparado con el grupo control, sin provocar reacciones adversas. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto https://revistas.unc.edu.ar/index.php/med/article/view/39035 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |