Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina
The Aichiviruses (AiV) belong to the Pircornaviridae family, genus Kobuvirus. Currently, 3 genotypes of AiV have been described: A, B and C. These viruses are transmitted via the fecal-oral route, possibly generating acute gastroenteritis in susceptible individuals. In the year 2021, the virus was d...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/38971 |
| Aporte de: |
| Sumario: | The Aichiviruses (AiV) belong to the Pircornaviridae family, genus Kobuvirus. Currently, 3 genotypes of AiV have been described: A, B and C. These viruses are transmitted via the fecal-oral route, possibly generating acute gastroenteritis in susceptible individuals. In the year 2021, the virus was detected in the sewage in the city of Córdoba which had been collected in the years 2013, 2018, 2019 and 2020 and this reflected a high circulation of the virus in the community. Objective: to detect and characterize the ahichivirus (AiV-1) genome in children with acute gastroenteritis in the city of Córdoba, Argentina.
One hundred and fifty-five samples of fecal matter (FM) from children with diarrhea were analyzed retrospectively. The children were assisted in a hospital in the city of Córdoba and the samples were obtained in the years 2013 (n= 13), 2014 (n=18), 2015 (n=15), 2016 (n=1), 2017 (n=1), 2018 (n=18) y 2019 (n=53). The clarification of the FM was performed at 10% with 0,02 M TRIS-HCL buffer (pH 7,2) followed by an extraction of nucleic acid with a ROCHE commercial kit. A nested RT-PCR was performed with primers directed to a conserved region of the viral genome, union region 3CD, of 266 bp. The positive samples were sequenced and analyzed phylogenetically.
The global frequency of detection of the AiV genome was 14.8% (23/155); 56.5% (13/23) of these samples were in monofection, 39.1% (9/23) were in coinfection with rotavirus and 0.4% (1/23) in coinfection with adenovirus, not only in hospitalized children but also in ambulatory patients aged from 0 to 8 years old, without significant differences between boys and girls. Eight amplicons were selected for sequencing and phylogenetic analysis. All the analyzed samples belonged to the B genotype with a high nucleotide identity (74% to 97%) between the isolated and the samples detected in the sewage.
This is the first work in Argentina which documents the detection of AiV in children with diarrhea and it challenges a deeper investigation of the etiologic participation of AiV in acute gastroenteritis condition. |
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