Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina
The Aichiviruses (AiV) belong to the Pircornaviridae family, genus Kobuvirus. Currently, 3 genotypes of AiV have been described: A, B and C. These viruses are transmitted via the fecal-oral route, possibly generating acute gastroenteritis in susceptible individuals. In the year 2021, the virus was d...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/38971 |
| Aporte de: |
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I10-R327-article-38971 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
Aichivirus Kobuvirus gastroenteritis nested RT-PCR B genotype Aichivirus Kobuvirus gastroenteritis RT-nested PCR genotipo B . |
| spellingShingle |
Aichivirus Kobuvirus gastroenteritis nested RT-PCR B genotype Aichivirus Kobuvirus gastroenteritis RT-nested PCR genotipo B . Peano, E Sicilia, P Cachi , AP Marinzalda, MA Castro , G Nates, S Masachessi, G Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina |
| topic_facet |
Aichivirus Kobuvirus gastroenteritis nested RT-PCR B genotype Aichivirus Kobuvirus gastroenteritis RT-nested PCR genotipo B . |
| author |
Peano, E Sicilia, P Cachi , AP Marinzalda, MA Castro , G Nates, S Masachessi, G |
| author_facet |
Peano, E Sicilia, P Cachi , AP Marinzalda, MA Castro , G Nates, S Masachessi, G |
| author_sort |
Peano, E |
| title |
Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina |
| title_short |
Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina |
| title_full |
Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina |
| title_fullStr |
Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina |
| title_full_unstemmed |
Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina |
| title_sort |
title: molecular detection and characterization of aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of córdoba, argentina |
| description |
The Aichiviruses (AiV) belong to the Pircornaviridae family, genus Kobuvirus. Currently, 3 genotypes of AiV have been described: A, B and C. These viruses are transmitted via the fecal-oral route, possibly generating acute gastroenteritis in susceptible individuals. In the year 2021, the virus was detected in the sewage in the city of Córdoba which had been collected in the years 2013, 2018, 2019 and 2020 and this reflected a high circulation of the virus in the community. Objective: to detect and characterize the ahichivirus (AiV-1) genome in children with acute gastroenteritis in the city of Córdoba, Argentina.
One hundred and fifty-five samples of fecal matter (FM) from children with diarrhea were analyzed retrospectively. The children were assisted in a hospital in the city of Córdoba and the samples were obtained in the years 2013 (n= 13), 2014 (n=18), 2015 (n=15), 2016 (n=1), 2017 (n=1), 2018 (n=18) y 2019 (n=53). The clarification of the FM was performed at 10% with 0,02 M TRIS-HCL buffer (pH 7,2) followed by an extraction of nucleic acid with a ROCHE commercial kit. A nested RT-PCR was performed with primers directed to a conserved region of the viral genome, union region 3CD, of 266 bp. The positive samples were sequenced and analyzed phylogenetically.
The global frequency of detection of the AiV genome was 14.8% (23/155); 56.5% (13/23) of these samples were in monofection, 39.1% (9/23) were in coinfection with rotavirus and 0.4% (1/23) in coinfection with adenovirus, not only in hospitalized children but also in ambulatory patients aged from 0 to 8 years old, without significant differences between boys and girls. Eight amplicons were selected for sequencing and phylogenetic analysis. All the analyzed samples belonged to the B genotype with a high nucleotide identity (74% to 97%) between the isolated and the samples detected in the sewage.
This is the first work in Argentina which documents the detection of AiV in children with diarrhea and it challenges a deeper investigation of the etiologic participation of AiV in acute gastroenteritis condition. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/38971 |
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I10-R327-article-389712024-04-15T16:14:45Z Title: Molecular detection and characterization of Aichivirus-1 in children assisted because of acute gastroenteritis in a hospital in the city of Córdoba, Argentina Detección y caracterización molecular de Aichivirus-1 en niños asistidos por gastroenteritis aguda en un nosocomio de la ciudad de Córdoba, Argentina. . Peano, E Sicilia, P Cachi , AP Marinzalda, MA Castro , G Nates, S Masachessi, G Aichivirus Kobuvirus gastroenteritis nested RT-PCR B genotype Aichivirus Kobuvirus gastroenteritis RT-nested PCR genotipo B . The Aichiviruses (AiV) belong to the Pircornaviridae family, genus Kobuvirus. Currently, 3 genotypes of AiV have been described: A, B and C. These viruses are transmitted via the fecal-oral route, possibly generating acute gastroenteritis in susceptible individuals. In the year 2021, the virus was detected in the sewage in the city of Córdoba which had been collected in the years 2013, 2018, 2019 and 2020 and this reflected a high circulation of the virus in the community. Objective: to detect and characterize the ahichivirus (AiV-1) genome in children with acute gastroenteritis in the city of Córdoba, Argentina. One hundred and fifty-five samples of fecal matter (FM) from children with diarrhea were analyzed retrospectively. The children were assisted in a hospital in the city of Córdoba and the samples were obtained in the years 2013 (n= 13), 2014 (n=18), 2015 (n=15), 2016 (n=1), 2017 (n=1), 2018 (n=18) y 2019 (n=53). The clarification of the FM was performed at 10% with 0,02 M TRIS-HCL buffer (pH 7,2) followed by an extraction of nucleic acid with a ROCHE commercial kit. A nested RT-PCR was performed with primers directed to a conserved region of the viral genome, union region 3CD, of 266 bp. The positive samples were sequenced and analyzed phylogenetically. The global frequency of detection of the AiV genome was 14.8% (23/155); 56.5% (13/23) of these samples were in monofection, 39.1% (9/23) were in coinfection with rotavirus and 0.4% (1/23) in coinfection with adenovirus, not only in hospitalized children but also in ambulatory patients aged from 0 to 8 years old, without significant differences between boys and girls. Eight amplicons were selected for sequencing and phylogenetic analysis. All the analyzed samples belonged to the B genotype with a high nucleotide identity (74% to 97%) between the isolated and the samples detected in the sewage. This is the first work in Argentina which documents the detection of AiV in children with diarrhea and it challenges a deeper investigation of the etiologic participation of AiV in acute gastroenteritis condition. Los Aichivirus (AiV) pertenecen a la familia Picornaviridae, género Kobuvirus. Al presente se han descripto 3 genotipos de AiV-1: A, B y C. Estos virus son trasmitidos por vía fecal-oral, pudiendo generar gastroenteritis aguda en individuos susceptibles. En el año 2021 se detectó el virus en las aguas residuales en la ciudad de Córdoba colectadas en los años 2013, 2018, 2019 y 2020 lo que reflejó una alta circulación del virus en la comunidad. Objetivo: detectar y caracterizar el genoma de aichivirus (AiV-1) en niños con gastroenteritis aguda en la ciudad de Córdoba, Argentina. Se analizaron retrospectivamente 155 muestras de materia (MF) fecal de niños con diarrea, asistidos en un nosocomio de la ciudad de Córdoba, obtenidas en los años 2013 (n= 13), 2014 (n=18), 2015 (n=15), 2016 (n=1), 2017 (n=1), 2018 (n=18) y 2019 (n=53). Se realizó la clarificación de la MF al 10% con buffer TRIS-HCL 0,02 M pH 7,2 seguido de una extracción de ácido nucleico con un kit comercial ROCHE. Se realizó una RT-nested PCR con primers dirigidos hacia una región conservada del genoma viral, región de unión 3CD, de 266 pb. Las muestras positivas fueron secuenciadas y analizadas filogenéticamente. La frecuencia global de detección del genoma de AiV fue del 14,8% (23/155) detectándose en el 56,5% (13/23) de las mismas en monoinfección, en el 39,1% (9/23) en co-infección con rotavirus y en el 0,4% (1/23) con adenovirus, tanto en niños internados como ambulatorios, en un rango etario de 0 meses a 8 años inclusive, sin diferencias significativas entre niños y niñas. Ocho amplicones fueron seleccionados para secuenciación y análisis filogenético. Todas las muestras analizadas pertenecieron al genotipo B con una alta identidad nucleotídica (74% al 97%) entre los aislados y las muestras detectadas en aguas residuales. Este es el primer trabajo en Argentina que documenta la detección de AiV en niños con diarrea y desafía a profundizar en la investigación sobre la participación etiológica de AiV en cuadros de gastroenteritis aguda. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto . https://revistas.unc.edu.ar/index.php/med/article/view/38971 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |