Evaluation of the antidepressant potential of the Hydroalcoholic extract of Glandularia Cabrerae (Moldenke) Botta in a Murine model
Depression is within the five most prevalent diseases in the world, affecting approximately 322 million people (Fusar-Poli L et al. 2019). Previous studies have shown that 80-90% of patients with major depressive disorder also have anxiety symptoms. Comorbidity between anxiety and depression predict...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/38881 |
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I10-R327-article-38881 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
Anxiety Disorders Major depression disorder Glandularia cabrerae ansiedad depresión Glandularia cabrerae . . |
| spellingShingle |
Anxiety Disorders Major depression disorder Glandularia cabrerae ansiedad depresión Glandularia cabrerae . . Robledo Almonacid, J Busajm, D López Quero, RM Vallejo , MG Carlini , V Evaluation of the antidepressant potential of the Hydroalcoholic extract of Glandularia Cabrerae (Moldenke) Botta in a Murine model |
| topic_facet |
Anxiety Disorders Major depression disorder Glandularia cabrerae ansiedad depresión Glandularia cabrerae . . |
| author |
Robledo Almonacid, J Busajm, D López Quero, RM Vallejo , MG Carlini , V |
| author_facet |
Robledo Almonacid, J Busajm, D López Quero, RM Vallejo , MG Carlini , V |
| author_sort |
Robledo Almonacid, J |
| title |
Evaluation of the antidepressant potential of the Hydroalcoholic extract of Glandularia Cabrerae (Moldenke) Botta in a Murine model |
| title_short |
Evaluation of the antidepressant potential of the Hydroalcoholic extract of Glandularia Cabrerae (Moldenke) Botta in a Murine model |
| title_full |
Evaluation of the antidepressant potential of the Hydroalcoholic extract of Glandularia Cabrerae (Moldenke) Botta in a Murine model |
| title_fullStr |
Evaluation of the antidepressant potential of the Hydroalcoholic extract of Glandularia Cabrerae (Moldenke) Botta in a Murine model |
| title_full_unstemmed |
Evaluation of the antidepressant potential of the Hydroalcoholic extract of Glandularia Cabrerae (Moldenke) Botta in a Murine model |
| title_sort |
evaluation of the antidepressant potential of the hydroalcoholic extract of glandularia cabrerae (moldenke) botta in a murine model |
| description |
Depression is within the five most prevalent diseases in the world, affecting approximately 322 million people (Fusar-Poli L et al. 2019). Previous studies have shown that 80-90% of patients with major depressive disorder also have anxiety symptoms. Comorbidity between anxiety and depression predicts poor outcomes with a higher percentage of treatment resistance (Coplan et al. 2015). There is a need for new investigations to develop novel drugsand strategies of treatments, which has led to alternative approaches within ethnomedicine, being our goal to study the effect of Glandularia Cabrerae (GC), native plant of Argentina; on the anxiety and depression behavior in a murine model. Therefore, the present study has as a hypothesis that the extract of this plant induces antidepressant predictive effect since it shares gender with Glandularia dissecta, popularly used in nervous system conditions .
Male albino Swiss-N: NIH mice, were treated with a hydroalcoholic extract of GC (macerated 5% w/v ethanol/water 1: 1) which was lyophilized and subsequently resuspected with saline solution. A set of 8 controls treated with saline solution was used, 8 animals treated with a dose of 55 mg/kg body weight and 8 with a dose of 175 mg/kg. The treatment for 28 days was carried out orally. An open field test (OFT) was performed to study depressive behavior. Setting basal, pre-treatment and post-treatment behavior. On the 28th day a Plus Maze test (PMT) was performed to assess anxiety. The OFT results were analyzed by repeated measures ANOVA and PMT by MANOVA.
The results show that there are no significant differences in the OFT, while the animals treated with both doses of the macerate exhibit a significant increase with respect to control group in the PMT, both in the %time in open arms (p = 0.00; F = 4.37; df = 18) as in the % entrance to open arms (p = 0.01 f = 4.37 df = 18). Although macerated has no antidepressant-like effect, it induces an anxiolytic effect that could contribute to the efficacy of depression treatment. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/38881 |
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I10-R327-article-388812024-04-15T16:14:45Z Evaluation of the antidepressant potential of the Hydroalcoholic extract of Glandularia Cabrerae (Moldenke) Botta in a Murine model Evaluación del potencial antidepresivo del extracto hidroalcohólico de Glandularia cabrerae (Moldenke) Botta en un modelo murino . Robledo Almonacid, J Busajm, D López Quero, RM Vallejo , MG Carlini , V Anxiety Disorders Major depression disorder Glandularia cabrerae ansiedad depresión Glandularia cabrerae . . Depression is within the five most prevalent diseases in the world, affecting approximately 322 million people (Fusar-Poli L et al. 2019). Previous studies have shown that 80-90% of patients with major depressive disorder also have anxiety symptoms. Comorbidity between anxiety and depression predicts poor outcomes with a higher percentage of treatment resistance (Coplan et al. 2015). There is a need for new investigations to develop novel drugsand strategies of treatments, which has led to alternative approaches within ethnomedicine, being our goal to study the effect of Glandularia Cabrerae (GC), native plant of Argentina; on the anxiety and depression behavior in a murine model. Therefore, the present study has as a hypothesis that the extract of this plant induces antidepressant predictive effect since it shares gender with Glandularia dissecta, popularly used in nervous system conditions . Male albino Swiss-N: NIH mice, were treated with a hydroalcoholic extract of GC (macerated 5% w/v ethanol/water 1: 1) which was lyophilized and subsequently resuspected with saline solution. A set of 8 controls treated with saline solution was used, 8 animals treated with a dose of 55 mg/kg body weight and 8 with a dose of 175 mg/kg. The treatment for 28 days was carried out orally. An open field test (OFT) was performed to study depressive behavior. Setting basal, pre-treatment and post-treatment behavior. On the 28th day a Plus Maze test (PMT) was performed to assess anxiety. The OFT results were analyzed by repeated measures ANOVA and PMT by MANOVA. The results show that there are no significant differences in the OFT, while the animals treated with both doses of the macerate exhibit a significant increase with respect to control group in the PMT, both in the %time in open arms (p = 0.00; F = 4.37; df = 18) as in the % entrance to open arms (p = 0.01 f = 4.37 df = 18). Although macerated has no antidepressant-like effect, it induces an anxiolytic effect that could contribute to the efficacy of depression treatment. La depresión se encuentra dentro de las cinco enfermedades más prevalentes del mundo, afectando aproximadamente a 322 millones de personas (Fusar-Poli L et al. 2019). Estudios previos han demostrado que el 80-90% de individuos con trastorno depresivo mayor tienen síntomas de ansiedad. La sobreposición de ansiedad y depresión tiene peores resultados con un porcentaje más alto de resistencia al tratamiento (Coplan et al. 2015). Esto genera la necesidad de nuevas investigaciones en búsqueda de un tratamiento más eficaz, lo que ha llevado indagar enfoques alternativos dentro de la etnomedicina, siendo nuestro objetivo estudiar el efecto de Glandularia Cabrerae (GC), planta nativa de Córdoba y La Rioja, sobre la conducta de ansiedad y depresión en un modelo murino. Nosotros hipotetizamos que el extracto de esta planta induce efecto predictivo antidepresivo dado que comparte género con Glandularia dissecta, usada popularmente en afecciones nerviosas. Ratones macho albinos Swiss-N:NIH , fueron tratados con un extracto hidroalcohólico de GC (macerado etanol/agua 1:1 al 5% p/v) el cual fue liofilizado y posteriormente resuspendido con solución fisiológica. Se empleó un set de 8 animales control tratados con solución fisiológica, 8 animales tratados con una dosis de 55 mg/kg peso corporal y 8 con una dosis de 175 mg/kg. El tratamiento durante 28 días se realizó por vía oral forzada. Los animales fueron expuestos al test de campo abierto (TCA) para estudiar la conducta de tipo depresiva, antes del inicio de tratamiento y al día 27. El día 28 se realizó el Test de laberinto elevado en cruz (LEC) para valorar ansiedad. Los resultados de TCA fueron analizados por ANOVA de medidas repetidas y los de LEC por MANOVA. Los resultados muestran que no hay diferencias significativas en el TCA, en tanto Los animales tratados con ambas dosis del macerado exhiben un incremento significativo respecto al grupo Control en el LEC, tanto en el %Tiempo en Brazos Abiertos (p =0,00; F=4,37; df=18) como en el % Entrada a Brazos Abiertos (p = 0,01 F=4,37 df=18). El macerado no presenta efecto antidepresivo, pero induce un efecto ansiolítico que podría contribuir a la eficacia del tratamiento de la depresión. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion . https://revistas.unc.edu.ar/index.php/med/article/view/38881 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |