Validation of the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of phenytoin in saliva at the Hospital de Niños de Santísima Trinidad.

Phenytoin (DFH), is an anticonvulsant widely used for the treatment of different types of seizures.1 Therapeutic monitoring (TDM) is required for DFH due to its narrow therapeutic range and nonlinear pharmacokinetics, among other characteristics. Monitoring is frequently done on plasma or serum (tot...

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Autores principales: Minetti, Araceli, Ducheins, Solange, Suarez, Hector Andres, Rivolta, Susana, Gonzalez, Isabel Inés
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023
Materias:
phenytoin
saliva
immunoassay
therapeutic monitoring
fenitoína
inmunoensayo
monitoreo terapéutico
imunoensaio
monitoramento terapêutico
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/37361
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-37361
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic phenytoin
saliva
immunoassay
therapeutic monitoring
fenitoína
saliva
inmunoensayo
monitoreo terapéutico
fenitoína
saliva
imunoensaio
monitoramento terapêutico
spellingShingle phenytoin
saliva
immunoassay
therapeutic monitoring
fenitoína
saliva
inmunoensayo
monitoreo terapéutico
fenitoína
saliva
imunoensaio
monitoramento terapêutico
Minetti, Araceli
Ducheins, Solange
Suarez, Hector Andres
Rivolta, Susana
Gonzalez, Isabel Inés
Validation of the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of phenytoin in saliva at the Hospital de Niños de Santísima Trinidad.
topic_facet phenytoin
saliva
immunoassay
therapeutic monitoring
fenitoína
saliva
inmunoensayo
monitoreo terapéutico
fenitoína
saliva
imunoensaio
monitoramento terapêutico
author Minetti, Araceli
Ducheins, Solange
Suarez, Hector Andres
Rivolta, Susana
Gonzalez, Isabel Inés
author_facet Minetti, Araceli
Ducheins, Solange
Suarez, Hector Andres
Rivolta, Susana
Gonzalez, Isabel Inés
author_sort Minetti, Araceli
title Validation of the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of phenytoin in saliva at the Hospital de Niños de Santísima Trinidad.
title_short Validation of the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of phenytoin in saliva at the Hospital de Niños de Santísima Trinidad.
title_full Validation of the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of phenytoin in saliva at the Hospital de Niños de Santísima Trinidad.
title_fullStr Validation of the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of phenytoin in saliva at the Hospital de Niños de Santísima Trinidad.
title_full_unstemmed Validation of the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of phenytoin in saliva at the Hospital de Niños de Santísima Trinidad.
title_sort validation of the immunological method of kinetic interaction of microparticles in solution (kims) for the determination of phenytoin in saliva at the hospital de niños de santísima trinidad.
description Phenytoin (DFH), is an anticonvulsant widely used for the treatment of different types of seizures.1 Therapeutic monitoring (TDM) is required for DFH due to its narrow therapeutic range and nonlinear pharmacokinetics, among other characteristics. Monitoring is frequently done on plasma or serum (total drug) through immunological methods. DFH can also be monitored in saliva, which shows a good correlation with plasma. The concentration of DFH in saliva reflects the concentration of free drug and due to the simplicity in its collection, it leads to a less stressful process for the patient. The aim of this study was to validate the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of DFH using saliva as biological matrix. Linearity, precision, detection and quantification limit, accuracy and interference were analyzed. Infostat 8.0 student version software was used for statistical analysis. The method was linear in a range between 0.41 and 5ug/ml. The detection and quantification limits were 0.14 and 0.45ug/ml, respectively. The equation of the straight line obtained based on the method comparison between KIMS and HPLC-UV was DFHKIMS= 0,81* DFHHPLC – 0,03.  The KIMS method proved to have the necessary analytical characteristics to be applied as a useful and practical tool for the follow-up of those patients with difficult venous access and/or children with chronic DFH treatments. 
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2023
url https://revistas.unc.edu.ar/index.php/med/article/view/37361
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spelling I10-R327-article-373612023-08-04T15:24:04Z Validation of the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of phenytoin in saliva at the Hospital de Niños de Santísima Trinidad. Validación del método inmunológico de interacción cinética de micropartículas en solución (KIMS) para la determinación de fenitoína en saliva en el hospital de Niños de la Santísima Trinidad. Validação do método imunológico de interação cinética de micropartículas em solução (KIMS) para a determinação de fenitoína em saliva no Hospital de Niños de la Santísima Trinidad. Minetti, Araceli Ducheins, Solange Suarez, Hector Andres Rivolta, Susana Gonzalez, Isabel Inés phenytoin saliva immunoassay therapeutic monitoring fenitoína saliva inmunoensayo monitoreo terapéutico fenitoína saliva imunoensaio monitoramento terapêutico Phenytoin (DFH), is an anticonvulsant widely used for the treatment of different types of seizures.1 Therapeutic monitoring (TDM) is required for DFH due to its narrow therapeutic range and nonlinear pharmacokinetics, among other characteristics. Monitoring is frequently done on plasma or serum (total drug) through immunological methods. DFH can also be monitored in saliva, which shows a good correlation with plasma. The concentration of DFH in saliva reflects the concentration of free drug and due to the simplicity in its collection, it leads to a less stressful process for the patient. The aim of this study was to validate the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of DFH using saliva as biological matrix. Linearity, precision, detection and quantification limit, accuracy and interference were analyzed. Infostat 8.0 student version software was used for statistical analysis. The method was linear in a range between 0.41 and 5ug/ml. The detection and quantification limits were 0.14 and 0.45ug/ml, respectively. The equation of the straight line obtained based on the method comparison between KIMS and HPLC-UV was DFHKIMS= 0,81* DFHHPLC – 0,03.  The KIMS method proved to have the necessary analytical characteristics to be applied as a useful and practical tool for the follow-up of those patients with difficult venous access and/or children with chronic DFH treatments.  La Fenitoína (DFH), es un anticonvulsivante ampliamente utilizado para el tratamiento de distintos tipos de convulsiones.1El monitoreo terapéutico (TDM) es requerido para la DFH debido a su estrecho rango terapéutico y farmacocinética no lineal, entre otras características. Los monitoreos se realizan frecuentemente en plasma o suero (droga total) a través de métodos inmunológicos. También se puede monitorear DFH en saliva, la cual presenta una buena correlación con el plasma. La concentración de DFH en saliva refleja la concentración de droga libre y debido a la simplicidad en su recolección, conlleva a un proceso menos estresante para el paciente. El objetivo del trabajo fue validar el método inmunológico de interacción cinética de micropartículas en solución (KIMS) para la determinación de DFH usando como matriz biológica saliva. Se analizó linealidad, precisión, límite de detección y cuantificación, exactitud e interferencia. Se utilizó el software Infostat 8.0 versión estudiantil para el análisis estadístico. El método fue lineal en un intervalo entre 0,41 y 5ug/ml. Los límites de detección y cuantificación fueron 0,14 y 0,45ug/ml respectivamente. La ecuación de la recta obtenida en base a la comparación de métodos entre KIMS y HPLC-UV fue DFHKIMS= 0,81* DFHHPLC – 0,03.  El método KIMS demostró tener las características analíticas necesarias paran ser aplicada como herramienta útil y práctica para el seguimiento de aquellos pacientes de difícil acceso venoso y/o niños con tratamientos crónicos con DFH.   Phenytoin (DFH), is an anticonvulsant widely used for the treatment of different types of seizures.1 Therapeutic monitoring (TDM) is required for DFH due to its narrow therapeutic range and nonlinear pharmacokinetics, among other characteristics. Monitoring is frequently done on plasma or serum (total drug) through immunological methods. DFH can also be monitored in saliva, which shows a good correlation with plasma. The concentration of DFH in saliva reflects the concentration of free drug and due to the simplicity in its collection, it leads to a less stressful process for the patient. The aim of this study was to validate the immunological method of kinetic interaction of microparticles in solution (KIMS) for the determination of DFH using saliva as biological matrix. Linearity, precision, detection and quantification limit, accuracy and interference were analyzed. Infostat 8.0 student version software was used for statistical analysis. The method was linear in a range between 0.41 and 5ug/ml. The detection and quantification limits were 0.14 and 0.45ug/ml, respectively. The equation of the straight line obtained based on the method comparison between KIMS and HPLC-UV was DFHKIMS= 0,81* DFHHPLC – 0,03.  The KIMS method proved to have the necessary analytical characteristics to be applied as a useful and practical tool for the follow-up of those patients with difficult venous access and/or children with chronic DFH treatments.  Keywords: diphenhydantoin; saliva; immunoassay; therapeutic monitoring  Resumo:  A fenitoína (DFH) é um anticonvulsivante amplamente utilizado para o tratamento de diversos tipos de convulsões. O monitoramento terapêutico (TDM) é necessário para DFH devido à sua estreita faixa terapêutica e farmacocinética não linear, entre outras características. A monitorização é frequentemente feita em plasma ou soro (droga total) através de métodos imunológicos. A DFH também pode ser monitorada na saliva, o que mostra uma boa correlação com o plasma. A concentração de DFH na saliva reflete a concentração do fármaco livre e devido à simplicidade em sua coleta, leva a um processo menos estressante para o paciente. O objetivo do trabalho foi validar o método imunológico de interação cinética de micropartículas em solução (KIMS) para a determinação de DFH utilizando saliva como matriz biológica. Foram analisadas linearidade, precisão, limite de detecção e quantificação, exatidão e interferência. O software Infostat 8.0 versão estudante foi utilizado para análise estatística. O método foi linear em uma faixa entre 0,41 e 5 ug/ml. Os limites de detecção e quantificação foram 0,14 e 0,45ug/ml, respectivamente. A equação da reta obtida com base na comparação de métodos entre KIMS e HPLC-UV foi DFHKIMS= 0,81* DFHHPLC – 0,03.  O método KIMS mostrou ter as características analíticas necessárias para ser aplicado como uma ferramenta útil e prática para o acompanhamento de pacientes com acesso venoso difícil e/ou crianças em tratamento crônico de DFH.  Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion text/html application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/37361 10.31053/1853.0605.v80.n2.37361 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 No. 2 (2023); 88-92 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 Núm. 2 (2023); 88-92 Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 n. 2 (2023); 88-92 1853-0605 0014-6722 10.31053/1853.0605.v80.n2 spa https://revistas.unc.edu.ar/index.php/med/article/view/37361/41784 https://revistas.unc.edu.ar/index.php/med/article/view/37361/41073 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0

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