Cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades
The purpose of this work was to obtain new data on factors intervening in the production and progression of human chagasiccardiopathy (HChC) with death by cardiac failure. Wc siudied cardiac samples ofpatients that died at an early mean age versus others that died at older ages. The infiltrating ce...
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| Autores principales: | , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2002
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/36780 |
| Aporte de: |
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I10-R327-article-36780 |
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| institution |
Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
Chagasic cardiopathy Intracardíac infiltrating cells Autoimmunity T-lymphocytes PAS T lymphocytes Macropages Mast cell Immunocellular factors of cardiomyocytes damage Poor cardiac perfomance Cardiac arrithmyas Cardiopatía chagásica Células infiltrantes intracardíacas Autoinmunidad LinfocitosT-linfocitos T PAS positivos Macrófagos Mastocitos Pobre perfomance cardíaca Arritmias cardíacas malignas |
| spellingShingle |
Chagasic cardiopathy Intracardíac infiltrating cells Autoimmunity T-lymphocytes PAS T lymphocytes Macropages Mast cell Immunocellular factors of cardiomyocytes damage Poor cardiac perfomance Cardiac arrithmyas Cardiopatía chagásica Células infiltrantes intracardíacas Autoinmunidad LinfocitosT-linfocitos T PAS positivos Macrófagos Mastocitos Pobre perfomance cardíaca Arritmias cardíacas malignas Cabral, Humberto Ivón , Novak Glocker, Mónica Castro Viera , Guido Cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades |
| topic_facet |
Chagasic cardiopathy Intracardíac infiltrating cells Autoimmunity T-lymphocytes PAS T lymphocytes Macropages Mast cell Immunocellular factors of cardiomyocytes damage Poor cardiac perfomance Cardiac arrithmyas Cardiopatía chagásica Células infiltrantes intracardíacas Autoinmunidad LinfocitosT-linfocitos T PAS positivos Macrófagos Mastocitos Pobre perfomance cardíaca Arritmias cardíacas malignas |
| author |
Cabral, Humberto Ivón , Novak Glocker, Mónica Castro Viera , Guido |
| author_facet |
Cabral, Humberto Ivón , Novak Glocker, Mónica Castro Viera , Guido |
| author_sort |
Cabral, Humberto |
| title |
Cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades |
| title_short |
Cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades |
| title_full |
Cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades |
| title_fullStr |
Cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades |
| title_full_unstemmed |
Cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades |
| title_sort |
cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades |
| description |
The purpose of this work was to obtain new data on factors intervening in the production and progression of human chagasiccardiopathy (HChC) with death by cardiac failure. Wc siudied cardiac samples ofpatients that died at an early mean age versus others that died at older ages. The infiltrating ce [1s were characterized and cluantified by means of specific monoclonal antibodies and inmunohisto- chemical methodology; and by classical histological and histochemical methods. We found intense cardiac infiltration by lymphocytes that reacted with the antibodies anti-CD45RO against activated T- lymphocytes (<70%). A similar number showed reactivity for C134. And, in seriate sections, showed PAS+ substances in their cytoplasms.Macrophages were detected in a number of 25%. Few lymphocytes reacted to C138. There was a low number of B lymphocytes. Other noticeable infiltrated eclI were intramiocardial mast cells. Qualitatively, such findings were similar in the two groups of patients. However, the number of T-lymphocytes and of the mast celis was significatively higher in the cases that underwent early cardiac death (p< 0.001). .1 cruzi was not found. In cardiomyocytes, damages were found, with T-lymphocytes and macrophages adhered to their sarcolemma, as weU as mast cells. CONCLUSIONS These findings suggest the existence of an active and intenseoccurrence of immunocellular mechanims in the production and evolution of severe HChC, in which T-lymphocytes CD4+ intervene, with production and secretion of PAS+ substances; Besides, macrophages, and mast cells, in that order. The quantity of infiltrated cells was positively associated with the occurrence of malignant arrithrnyas. We suggest that such facts would be involved in the poor cardiac perfomance of the chagasic heart disease that leads to cardiac death. Indeed, they seem be worthy of consideration with respect to therapeutic strategies. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2002 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/36780 |
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I10-R327-article-367802022-03-15T21:10:09Z Cardiopatía chagásica: identificación y cuantificación de las células infiltrantes, de corazones de pacientes que sufrieron muerte cardíaca a distintas edades Cabral, Humberto Ivón , Novak Glocker, Mónica Castro Viera , Guido Chagasic cardiopathy Intracardíac infiltrating cells Autoimmunity T-lymphocytes PAS T lymphocytes Macropages Mast cell Immunocellular factors of cardiomyocytes damage Poor cardiac perfomance Cardiac arrithmyas Cardiopatía chagásica Células infiltrantes intracardíacas Autoinmunidad LinfocitosT-linfocitos T PAS positivos Macrófagos Mastocitos Pobre perfomance cardíaca Arritmias cardíacas malignas The purpose of this work was to obtain new data on factors intervening in the production and progression of human chagasiccardiopathy (HChC) with death by cardiac failure. Wc siudied cardiac samples ofpatients that died at an early mean age versus others that died at older ages. The infiltrating ce [1s were characterized and cluantified by means of specific monoclonal antibodies and inmunohisto- chemical methodology; and by classical histological and histochemical methods. We found intense cardiac infiltration by lymphocytes that reacted with the antibodies anti-CD45RO against activated T- lymphocytes (<70%). A similar number showed reactivity for C134. And, in seriate sections, showed PAS+ substances in their cytoplasms.Macrophages were detected in a number of 25%. Few lymphocytes reacted to C138. There was a low number of B lymphocytes. Other noticeable infiltrated eclI were intramiocardial mast cells. Qualitatively, such findings were similar in the two groups of patients. However, the number of T-lymphocytes and of the mast celis was significatively higher in the cases that underwent early cardiac death (p< 0.001). .1 cruzi was not found. In cardiomyocytes, damages were found, with T-lymphocytes and macrophages adhered to their sarcolemma, as weU as mast cells. CONCLUSIONS These findings suggest the existence of an active and intenseoccurrence of immunocellular mechanims in the production and evolution of severe HChC, in which T-lymphocytes CD4+ intervene, with production and secretion of PAS+ substances; Besides, macrophages, and mast cells, in that order. The quantity of infiltrated cells was positively associated with the occurrence of malignant arrithrnyas. We suggest that such facts would be involved in the poor cardiac perfomance of the chagasic heart disease that leads to cardiac death. Indeed, they seem be worthy of consideration with respect to therapeutic strategies. Esta investigación fue realizada para obtener datos nuevos sobre factores que intervendrían en la Producción Y progresión de la cardiopatía chagásica humana (CChiI) con muerte por fallo cardíaco. Estudiamos mues- tras cardíacas de pacientes que murieron a edad temprana, y más tardía. METODOS: Caracterizamos y cuantificamos los tipos de células infiltrantes mediante anticuerposmonoclonales; y con métodos histológicos clásicos. RESULTADOS: Hallamos intensa infiltración intracardfaca por linfocitos quereaccionaron conel anticuerpo paraCD45RO que muestra linfocitos T activados (más de 701/(;). Similar número mostró reactividad para subtipo C134. Dichos linfocitos mostraron substancias PAS+ en citoplasma. Macrófagos fueron detectados en número superior a 25%. Pocos linfocitos reaccionaron para CDS. Hubo un número muy bajo de linfocitos B. Otras células infiltradas fueron mastocitosintramiocárdicos, en número elevado. Cualitativamente, esos hallazgos fueron similares en los dos grupos de pacientes.Cuantitativamente, el número de linfocitos T y el de mastocitos fue significativamente mayor en los casos que sufrieron muerte temprana (p< 0.001). No se observó Tcri.czi. Se observaron daños en cardiomiocitos, con linfocitos T y macrófagos adheridos. CONCLUSIONES: Los hallazgos sugieren una activa e intensa existencia de mecanismos inmunocelulares en la producción y evolución de la CChII grave, en los que intervienen linfocitos T CD4+, con substancias PAS+, macrófagos, y mastocitos. Dichas células, mostraron citoadherencia a cardiomiocitos con lesiones; y por su ubicación y número asociáronse también a arritmias cardíacas malignas. En base a dichos hechos sugerimos que serían causas de pobre perfomance cardíaca en la CChH, y merecerían considerarse con respecto a estrategias terapéuticas. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2002-03-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/36780 10.31053/1853.0605.v59.n1.36780 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 59 No. 1 (2002); 83-89 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 59 Núm. 1 (2002); 83-89 Revista da Faculdade de Ciências Médicas de Córdoba; v. 59 n. 1 (2002); 83-89 1853-0605 0014-6722 10.31053/1853.0605.v59.n1 spa https://revistas.unc.edu.ar/index.php/med/article/view/36780/37128 Derechos de autor 2022 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0 |